LOCUS       pBAC-6.        5580 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the gp64 promoter, encoding an 
            N-terminal signal sequence-6xHis-thrombin-S-Tag-enterokinase 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC-6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1166..1371
                     /label=gp64 promoter
                     /note="promoter for the baculovirus gp64 glycoprotein gene"
     sig_peptide     1372..1431
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     CDS             1441..1458
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1468..1485
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1489..1533
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1549..1563
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1565..1629
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1630..1647
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     1684..3041
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3304..3892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4066..4923)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4924..5028)
                     /label=AmpR promoter
     rep_origin      5055..5510
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgtcg actgagcgtc cgtgttcatg atcccgtttt
     1201 tataacagcc agataaaaat aatcttatca attaagataa aaagataaga ttattaatct
     1261 aacaacgtgc cttgtgtcac gtaggccaga taacggtcgg gtatataaga cgcctcaacg
     1321 ctactagtaa atcagtcaca ccaaggcttc aataaggaac acacaagcaa gatggtaagc
     1381 gctattgttt tatatgtgct tttggcggcg gcggcgcatt ctgcctttgc ggccatggtc
     1441 catcatcacc accatcactc cgcgggtttg gtgcccagag gcagcggcaa agaaacggcg
     1501 gcggcgaaat ttgaacgcca acacatggac agcgctagcg gtggaggtga tgacgacgac
     1561 aagagcccgg gcttctcctc aaaaggcctg gatccgaatt cgagctcgaa gcttgcggcc
     1621 gcactcgagc accaccacca ccaccactaa cctaggtagc tgagcgcatg caagctgatc
     1681 cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt
     1741 gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga
     1801 aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt
     1861 tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac
     1921 tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag
     1981 ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat
     2041 tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca
     2101 cgttaaatag agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat
     2161 cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca
     2221 cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt
     2281 ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt
     2341 ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca
     2401 aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag
     2461 gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag
     2521 gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg
     2581 ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca
     2641 acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag
     2701 cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca
     2761 cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc
     2821 gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc
     2881 gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat
     2941 ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa
     3001 tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata
     3061 acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgagg
     3121 tttaaacgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3721 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3901 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3961 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4021 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4081 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4141 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4201 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4261 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4321 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4381 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4441 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4501 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4561 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4621 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4681 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4741 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4801 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4861 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4921 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4981 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5041 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5101 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5161 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5221 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5281 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5341 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5401 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5461 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc
     5521 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
//
