LOCUS       pBAC-5.        5517 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the gp64 promoter, encoding an 
            N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1166..1369
                     /label=gp64 promoter
                     /note="promoter for the baculovirus gp64 glycoprotein gene"
     CDS             1372..1374
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1378..1395
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1405..1422
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1426..1470
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1486..1500
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1502..1566
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1567..1584
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     1621..2978
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3241..3829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4003..4860)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4861..4965)
                     /label=AmpR promoter
     rep_origin      4992..5447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgtcg actgagcgtc cgtgttcatg atcccgtttt
     1201 tataacagcc agataaaaat aatcttatca attaagataa aaagataaga ttattaatct
     1261 aacaacgtgc cttgtgtcac gtaggccaga taacggtcgg gtatataaga cgcctcaacg
     1321 ctactagtaa atcagtcaca ccaaggcttc aataaggaac acacaagcac catggtccat
     1381 catcaccacc atcactccgc gggtttggtg cccagaggca gcggcaaaga aacggcggcg
     1441 gcgaaatttg aacgccaaca catggacagc gctagcggtg gaggtgatga cgacgacaag
     1501 agcccgggct tctcctcaaa aggcctggat ccgaattcga gctcgaagct tgcggccgca
     1561 ctcgagcacc accaccacca ccactaacct aggtagctga gcgcatgcaa gctgatccgg
     1621 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     1681 gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa
     1741 tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt
     1801 aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat
     1861 ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt
     1921 tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat
     1981 gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt
     2041 taaatagagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt
     2101 cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac
     2161 gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg
     2221 gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta
     2281 attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat
     2341 ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg
     2401 gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct
     2461 caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg
     2521 tttttggttt gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca
     2581 attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg
     2641 gcggcaattc agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg
     2701 gagaaggtgg tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca
     2761 cgattgtggg caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag
     2821 gcagcgcttg gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg
     2881 ctataagcat tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt
     2941 aagcaattgt attgtaaaga gattgtctca agctcggatc gatcccgcac gccgataaca
     3001 agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgaggttt
     3061 aaacgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4981 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5401 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt
     5461 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgcca
//
