LOCUS       pBAC-3.        5474 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the polyhedrin promoter, encoding 
            an N-terminal signal sequence-6xHis-thrombin-S-Tag-enterokinase 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1168..1259
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     1266..1325
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     CDS             1335..1352
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1362..1379
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1383..1427
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1443..1457
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1460..1523
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1524..1541
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     1578..2935
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3198..3786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3960..4817)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4818..4922)
                     /label=AmpR promoter
     rep_origin      4949..5404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aaccatctcg
     1201 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     1261 tgcccatgtt aagcgctatt gttttatatg tgcttttggc ggcggcggcg cattctgcct
     1321 ttgcggccat ggtccatcat caccaccatc actccgcggg tttggtgccc agaggcagcg
     1381 gcaaagaaac ggcggcggcg aaatttgaac gccaacacat ggacagcgct agcggtggag
     1441 gtgatgacga cgacaagagc ccgggcttct cctcaaaagg cctggatccg aattcgagct
     1501 cgaagcttgc ggccgcactc gagcaccacc accaccacca ctaacctagg tagctgagcg
     1561 catgcaagct gatccgggtt attagtacat ttattaagcg ctagattctg tgcgttgttg
     1621 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
     1681 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
     1741 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
     1801 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
     1861 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
     1921 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
     1981 actcgacgta aacacgttaa atagagcttg gacatattta acatcgggcg tgttagcttt
     2041 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
     2101 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
     2161 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
     2221 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
     2281 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
     2341 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
     2401 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
     2461 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
     2521 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
     2581 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
     2641 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
     2701 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
     2761 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
     2821 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
     2881 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat
     2941 cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt
     3001 cgctgtcgtc gaggtttaaa cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3061 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3601 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3961 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4021 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4081 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4141 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4201 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4261 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4321 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4561 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4921 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     4981 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5041 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5101 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5161 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5221 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5281 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5341 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5401 atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     5461 tcgctattac gcca
//
