LOCUS       V000980                 6600 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000980
VERSION     V000980
KEYWORDS    pBABe-HA-ER-IPpoI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Berkovich E, Monnat RJ Jr, Kastan MB
  TITLE     Roles of ATM and NBS1 in chromatin structure modulation and DNA
            double-strand break repair.
  JOURNAL   Nat Cell Biol. 2007 Jun;9(6):683-90. Epub 2007 May 7.
   PUBMED   17486112
REFERENCE   2  (bases 1 to 6600)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell
            Biol."; date: "2007-06"; volume: "9(6)"; pages: "683-90. Epub 2007
            May 7"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    542..741
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             942..1358
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1386..1412
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1800..2162
                     /gene="ESR1"
                     /label="Estrogen receptor"
                     /note="Estrogen receptor from Macaca mulatta. Accession#:
                     P49886"
     promoter        2935..3264
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3274..3870
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             3972..4563
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(4693..4710)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4864..5452)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5615..6472)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6473..6577)
                     /label="AmpR promoter"
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatcatc gaattcacca
     1381 tggcataccc atacgatgtt ccagattacg ctagcggatc tcgaaatgaa atgggtgctt
     1441 caggagacat gagggctgcc aacctttggc caagccctct tgtgattaag cacactaaga
     1501 agaatagccc tgccttgtcc ttgacagctg accagatggt cagtgccttg ttggatgctg
     1561 aaccgcccat gatctattct gaatatgatc cttctagacc cttcagtgaa gcctcaatga
     1621 tgggcttatt gaccaaccta gcagataggg agctggttca tatgatcaac tgggcaaaga
     1681 gagtgccagg ctttggggac ttgaatctcc atgatcaggt ccaccttctc gagtgtgcct
     1741 ggctggagat tctgatgatt ggtctcgtct ggcgctccat ggaacacccg gggaagctcc
     1801 tgtttgctcc taacttgctc ctggacagga atcaaggtaa atgtgtggaa ggcatggtgg
     1861 agatctttga catgttgctt gctacgtcaa gtcggttccg catgatgaac ctgcagggtg
     1921 aagagtttgt gtgcctcaaa tccatcattt tgcttaattc cggagtgtac acgtttctgt
     1981 ccagcacctt gaagtctctg gaagagaagg accacatcca ccgtgtcctg gacaagatca
     2041 cagacacttt gatccacctg atggccaaag ctggcctgac tctgcagcag cagcatcgcc
     2101 gcctagctca gctccttctc attctttccc atatccggca tatgagtaac aaacgcatgg
     2161 agcatctcta caacatgaaa tgcaagaacg tggtacccct ctatgacctg ctcctggaga
     2221 tgttggatgc ccaccgcctt catgccccag ccagtcgcat gggagtgccc ccagaggagc
     2281 ccagccagac ccagctggcc accaccagct ccacttcagc acattcctta caaacctact
     2341 acataccccc ggaagcagag ggcttcccca acacgatcgg atctatggcg ctcaccaatg
     2401 ctcaaatctt ggctgtgatt gacagctggg aagaaacagt cggtcagttt ccagtgataa
     2461 cgcaccatgt accattaggt ggcggtctgc aaggaacgct ccattgttac gagatccccc
     2521 tagcagctcc ttatggggtt ggctttgcta agaatgggcc tacccgctgg caatacaaac
     2581 ggacaatcaa tcaagtcgtc cacagatggg gatcccacac agtccctttt ctattagaac
     2641 cggataacat caacggcaaa acctgcacag catcgcacct atgtcataat actcgatgcc
     2701 acaatccctt gcacttgtgc tgggagtcac tagacgacaa caaaggcaga aactggtgcc
     2761 cgggtcccaa cgggggatgt gtccatgcgg tggtttgttt aaggcagggt ccgttgtacg
     2821 gcccgggggc gactgtggca ggtcctcaac aaaggggcag tcactttgtg gtataaagat
     2881 cccagtgtgg tggtacgtag gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt
     2941 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3241 tttggaggcc taggcttttg caaaaagctt accatgaccg agtacaagcc cacggtgcgc
     3301 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     3361 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     3421 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3481 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     3541 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     3601 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     3661 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     3721 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     3781 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     3841 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     3901 accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt tagtctccag
     3961 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     4021 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     4081 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     4141 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     4201 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     4261 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     4321 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     4381 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
     4441 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     4501 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     4561 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg
     4621 gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5281 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgc aaatcaatct
     5581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6541 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
