LOCUS       62056_3450        8482 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             942..1358
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     promoter        4817..5146
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5156..5752
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             5854..6445
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(6746..7334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7497..8354)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8355..8459)
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acggccgcgg gaattcgatt ggaagatgcc ccggccggag ctgcccctgc cggagggctg
     1441 ggaggaggcg cgcgacttcg acggcaaggt ctactacata gaccacacga accgcaccac
     1501 cagctggatc gacccgcggg acaggtacac caaaccgctc acctttgctg actgcattag
     1561 tgatgagttg ccgctaggat gggaagaggc atatgaccca caggttggag attacttcat
     1621 agaccacaac accaaaacca ctcagattga ggatcctcga gtacaatggc ggcgggagca
     1681 ggaacatatg ctgaaggatt acctggtggt ggcccaggag gctctgagtg cacaaaagga
     1741 gatctaccag gtgaagcagc agcgcctgga gcttgcacag caggagtacc agcaactgca
     1801 tgccgtctgg gagcataagc tgggctccca ggtcagcttg gtctctggtt catcatccag
     1861 ctccaagtat gaccctgaga tcctaaaagc tgaaattgcc actgcaaaat cccgggtcaa
     1921 caagctgaag agagagatgg ttcacctcca gcacgagctg cagttcaaag agcgtggctt
     1981 tcagaccctg aagaaaatcg ataagaaaat gtctgatgct cagggcagct acaaactgga
     2041 tgaagctcag gctgtcttga gagaaacaaa agccatcaaa aaggctatta cctgtgggga
     2101 aaaggaaaag caagatctca ttaagagcct tgccatgttg aaggacggct tccgcactga
     2161 cagggggtct cactcagacc tgtggtccag cagcagctct ctggagagtt cgagtttccc
     2221 gctaccgaaa cagtacctgg atgtgagctc ccagacagac atctcgggaa gcttcggcat
     2281 caacagcaac aatcagttgg cagagaaggt cagattgcgc cttcgatatg aagaggctaa
     2341 gagaaggatc gccaacctga agatccagct ggccaagctt gacagtgagg cctggcctgg
     2401 ggtgctggac tcagagaggg accggctgat ccttatcaac gagaaggagg agctgctgaa
     2461 ggagatgcgc ttcatcagcc cccgcaagtg gacccagggg gaggtggagc agctggagat
     2521 ggcccggaag cggctggaaa aggacctgca ggcagcccgg gacacccaga gcaaggcgct
     2581 gacggagagg ttaaagttaa acagtaagag gaaccagctt gtgagagaac tggaggaagc
     2641 cacccggcag gtggcaactc tgcactccca gctgaaaagt ctctcaagca gcatgcagtc
     2701 cctgtcctca ggcagcagcc ccggatccct cacgtccagc cggggctccc tggttgcatc
     2761 cagcctggac tcctccactt cagccagctt cactgacctc tactatgacc cctttgagca
     2821 gctggactta gagctgcaga gcaaggtgga gttcctgctc ctggaggggg ccaccggctt
     2881 ccggccctca ggctgcatca ccaccatcca cgaggatgag gtggccaaga cccagaaggc
     2941 agagggaggt ggccgcctgc aggctctgcg ttccctgtct ggcaccccaa agtccatgac
     3001 ctccctatcc ccacgttcct ctctctcctc cccctcccca ccctgttccc ctctcatggc
     3061 tgaccccctc ctggctggtg atgccttcct caactccttg gagtttgaag acccggagct
     3121 gagtgccact ctttgtgaac tgagccttgg taacagcgcc caggaaagat accggctgga
     3181 ggaaccagga acggagggca agcagctggg ccaagctgtg aatacggccc aggggtgtgg
     3241 cctgaaagtg gcctgtgtct cagccgccgt atcggacgag tcagtggctg gagacagtgg
     3301 tgtgtacgag gcttccgtgc agagactggg tgcttcagaa gctgctgcat ttgacagtga
     3361 cgaatcggaa gcagtgggtg cgacccgaat tcagattgcc ctgaagtatg atgagaagaa
     3421 taagcaattt gcaatattaa tcatccagct gagtaacctt tctgctctgt tgcagcaaca
     3481 agaccagaaa gtgaatatcc gcgtggctgt ccttccttgc tctgaaagca caacctgcct
     3541 gttccggacc cggcctctgg acgcctcaga cactctagtg ttcaatgagg tgttctgggt
     3601 atccatgtcc tatccagccc ttcaccagaa gaccttaaga gtcgatgtct gtaccaccga
     3661 caggagccat ctggaagagt gcctgggagg cgcccagatc agcctggcgg aggtctgccg
     3721 gtctggggag aggtcgactc gctggtacaa ccttctcagc tacaaatact tgaagaagca
     3781 gagcagggag ctcaagccag tgggagttat ggcccctgcc tcagggcctg ccagcacgga
     3841 cgctgtgtct gctctgttgg aacagacagc agtggagctg gagaagaggc aggagggcag
     3901 gagcagcaca cagacactgg aagacagctg gaggtatgag gagaccagtg agaatgaggc
     3961 agtagccgag gaagaggagg aggaggtgga ggaggaggag ggagaagagg atgttttcac
     4021 cgagaaagcc tcacctgata tggatgggta cccagcatta aaggtggaca aagagaccaa
     4081 cacggagacc ccggccccat cccccacagt ggtgcgacct aaggaccgga gagtgggcac
     4141 cccgtcccag gggccatttc ttcgagggag caccatcatc cgctctaaga ccttctcccc
     4201 aggaccccag agccagtacg tgtgccggct gaatcggagt gatagtgaca gctccactct
     4261 gtccaaaaag ccaccttttg ttcgaaactc cctggagcga cgcagcgtcc ggatgaagcg
     4321 gccttcctcg gtcaagtcgc tgcgctccga gcgtctgatc cgtacctcgc tggacctgga
     4381 gttagacctg caggcgacaa gaacctggca cagccaattg acccaggaga tctcggtgct
     4441 gaaggagctc aaggagcagc tggaacaagc caagagccac ggggagaagg agctgccaca
     4501 gtggttgcgt gaggacgagc gtttccgcct gctgctgagg atgctggaga agcggatgga
     4561 ccgagcggag cacaagggtg agcttcagac agacaagatg atgagggcag ctgccaagga
     4621 tgtgcacagg ctccgaggcc agagctgtaa ggaaccccca gaagttcagt ctttcaggga
     4681 gaagatggca tttttcaccc ggcctcggat gaatatccca gctctctctg cagatgacgt
     4741 ctaatcgcca gaaaagaatc actagtgaat tcgcggccgt aggaattcgc cagcacagtg
     4801 gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4981 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5041 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5101 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatgac
     5161 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     5221 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     5281 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5341 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     5401 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     5461 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     5521 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     5581 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     5641 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     5701 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     5761 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgataaaata
     5821 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     5881 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     5941 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     6001 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     6061 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     6121 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     6181 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     6241 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     6301 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     6361 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     6421 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
     6481 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga
     6541 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6601 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6661 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6721 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6781 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6841 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6901 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6961 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7021 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7081 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7141 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7201 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7261 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7321 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7381 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7441 ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8461 acatttcccc gaaaagtgcc ac
//
