LOCUS       pBABE-Zeo.        4888 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral expression vector derived from the Moloney murine 
            leukemia virus. Contains a Zeocin(TM) resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-Zeo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Morgenstern JP, Land H.
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990;18:3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Cell Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990"; volume: "18"; pages: "3587-96"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Strong expression is driven by the viral promoter in the 5' LTR. The
            commercially provided sequence was modified to include the splice 
            donor mutation.
FEATURES             Location/Qualifiers
     source          1..4888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..479
                     /label=LTR (truncated)
                     /note="LTR (truncated)"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    541
                     /label=splice donor mutation
                     /note="splice donor mutation"
                     /note="T-to-C mutation that blocks RNA splicing"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1355..1402
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1413..1742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1749..2120
                     /label=BleoR
                     /note="antibiotic-binding protein"
     LTR             2169..2762
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(3134..3722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3903..4760)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4761..4865)
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
     1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1741 aaaagcttat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     1801 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     1861 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     1921 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     1981 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     2041 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     2101 tcgtggccga ggagcaggac tgaatcgata aaataaaaga ttttatttag tctccagaaa
     2161 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     2221 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
     2281 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     2341 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     2401 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     2461 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     2521 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     2581 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     2641 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     2701 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     2761 tttccgactt gtggtctcgc tgccttggga gggtctcctc tgagtgattg actacccgtc
     2821 agcgggggtc ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
     2881 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
     2941 cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3541 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3841 aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4501 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4861 ccgcgcacat ttccccgaaa agtgccac
//
