LOCUS       pBABE-Hygro.        5558 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral expression vector derived from the Moloney murine 
            leukemia virus. Contains a hygromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-Hygro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Morgenstern JP, Land H.
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990;18:3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Cell Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5558)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990"; volume: "18"; pages: "3587-96"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Strong expression is driven by the viral promoter in the 5' LTR. The
            commercially provided sequence was modified to include the splice 
            donor mutation.
FEATURES             Location/Qualifiers
     source          1..5558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    541
                     /label=splice donor mutation
                     /note="splice donor mutation"
                     /note="T-to-C mutation that blocks RNA splicing"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1355..1402
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1413..1742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1770..2792
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     LTR             2839..3432
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(3804..4392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4573..5430)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5431..5535)
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
     1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1741 aaaagcttac catgaccgag tacaagccca tgaaaaagcc tgaactcacc gcgacgtctg
     1801 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     1861 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     1921 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     1981 cgctcccgat tccggaagtg cttgacattg gggagttcag cgagagcctg acctattgca
     2041 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     2101 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     2161 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     2221 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     2281 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     2341 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     2401 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     2461 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     2521 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     2581 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     2641 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     2701 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     2761 gacgccccag cactcgtccg agggcaaagg aatatcgata aaataaaaga ttttatttag
     2821 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     2881 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     2941 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     3001 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     3061 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     3121 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     3181 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     3241 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     3301 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     3361 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     3421 gggtctttca tttccgactt gtggtctcgc tgccttggga gggtctcctc tgagtgattg
     3481 actacccgtc agcgggggtc ttcacatgca gcatgtatca aaattaattt ggtttttttt
     3541 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
     3601 aggcggtttg cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4201 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4501 ttaaattaaa aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg
     4561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5161 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     5221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5521 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
