LOCUS       62056_3435       10898 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             290..1078
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1325..1913
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2099..2239)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2583..2777)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2846..3910)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(4347..4973)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(6273..6297)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        6977..7321
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7430..7554
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        7579..7609
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             7663..8319
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             8664..8966
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(9010..9134)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9501..9678
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9721..10269
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIQTSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      10292..10543
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(10636..10660)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
      181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt
     1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
     6361 agctctccca tatggtcgag atntcctttg ccccggagat caccatggac gactttctct
     6421 atctctacga tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg
     6481 ataatgagaa gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag
     6541 aggcctacgc ggcaggtctc atcaagacga tctacccgag taataatctc caggagatca
     6601 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga
     6661 acacagagaa agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
     6721 gcttgcttca taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta
     6781 ctgaatcaaa ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga
     6841 agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg
     6901 tcaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     6961 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     7021 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     7081 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
     7141 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     7201 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
     7261 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     7321 ctgcaggacg atccgtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat
     7381 tacaatttac tattctagtc gacctgcagg cggccgcact agtgatatca caagtttgta
     7441 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     7501 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     7561 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     7621 agttaggatc cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     7681 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     7741 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     7801 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     7861 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     7921 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     7981 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     8041 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     8101 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     8161 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     8221 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     8281 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     8341 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     8401 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     8461 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     8521 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     8581 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     8641 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     8701 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     8761 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     8821 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     8881 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     8941 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     9001 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     9061 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     9121 ttgtacaaag tggtgatatc ccgcggccat gctagagtcc gcaaaaatca ccagtctctc
     9181 tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa
     9241 gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     9301 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     9361 cagtgacctg caggcatgcg acgtcgggcc ctctagagga tccccgggta ccgcgaatta
     9421 tcatacatga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg ggacaagccg
     9481 ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc gggtttctgg
     9541 agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa
     9601 ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt
     9661 cccctcggta tccaattaga gtctcatatt cactctcaac tcgatcgagg gggatctacc
     9721 atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg
     9781 gtctgcacca tcgtcaacca ctacatccag acaagcacgg tcaacttccg taccgagccg
     9841 caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc
     9901 gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggtccctg gaaggcacgc
     9961 aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg
    10021 ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag
    10081 agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc
    10141 ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac
    10201 gtgggtttct ggcagctgga cttcagcctg ccggtgccgc cccgtccggt cctgcccgtc
    10261 accgaaatct gatgacccct agagtcaagc agatcgttca aacatttggc aataaagttt
    10321 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
    10381 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggttttttg
    10441 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
    10501 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg
    10561 ataattcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
    10621 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
    10681 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
    10741 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
    10801 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
    10861 tgattgtaac gatgacagag cgttgctgcc tgtgatca
//
