LOCUS       V012168                 3966 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V012168
VERSION     V012168
KEYWORDS    pB1H2w2-zif268
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 Mar 10. ():.
   PUBMED   18332042
REFERENCE   2  (bases 1 to 3966)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. 2008 Mar 10. ():."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    50..66
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             306..575
                     /label="RNAP omega"
                     /note="DNA-directed RNA polymerase subunit omega"
     CDS             585..608
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             672..848
                     /gene="EGR1"
                     /label="Early growth response protein 1"
                     /note="Early growth response protein 1 from Serinus
                     canaria. Accession#: O73694"
     rep_origin      1027..1482
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1508..1612
                     /label="AmpR promoter"
     CDS             1613..2470
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2644..3232
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     3386..3403
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     misc_feature    complement(3418..3558)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     primer_bind     3644..3666
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3663..3851)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gaattccggg ctttacactt tatgcttccg gctcgcatta tgtgtcgact tgtgagcgga
       61 taacaatttc acacaggaaa cagctatgcc tcaacagcag caaatgcaac ctcccaattc
      121 aagtgcggac aacaaccctt tgcaacagca atcatcacaa aataccgtac caaacgtcct
      181 caaccaaatt aaccaaatct tttctccaga ggagcaacgc agcttattac aagaagccat
      241 cgaaacctgc aagaattttg aaaaaacaca attgtaagtc tcggctccaa gaaggagata
      301 tacccatggc acgcgtaact gttcaggacg ctgtagagaa aattggtaac cgttttgacc
      361 tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt aggcggaaag gacccgctcg
      421 taccggaaga aaacgataaa accactgtaa tcgcgctgcg cgaaatcgaa gaaggtctga
      481 tcaacaacca gatcctcgac gttcgcgaac gccaggaaca gcaagagcag gaagccgctg
      541 aattacaagc cgttaccgct attgctgaag gtcgtgcggc cgcggactac aaggatgacg
      601 acgacaagtt ccggaccggt tccaagacac ccccccatgg tacccgccca tatgcttgcc
      661 ctgtcgagtc ctgcgatcgc cgcttttctc gctcggatga gcttacccgc catatccgca
      721 tccataccgg tcagaagccc ttccagtgtc gaatctgcat gcgtaacttc agtcgtagtg
      781 accaccttac cacccacatc cgcacccaca caggcgagaa gccttttgcc tgtgacattt
      841 gtgggaggaa gtttgccagg agtgatgaac gcaagaggca taccaaaatc catttaagac
      901 agaaggacgt ctagagacta gaaaaaggcc gacaagtccc gctccgctga agatcctggc
      961 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1021 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1081 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1201 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1321 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1381 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1441 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1621 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1681 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1741 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1801 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1861 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1921 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1981 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2041 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2101 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2161 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2221 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2281 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2341 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2401 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2461 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2521 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2761 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     2821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     3421 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     3481 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     3541 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3601 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3661 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     3721 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     3781 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     3841 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     3901 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     3961 gaacgg
//
