LOCUS       pAviTag_N-His_Ka        2358 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for Expresso(R) cloning to add N-terminal 6xHis and 
            biotinylation tags, with rhamnose-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    pAviTag N-His Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     RBS             153..175
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             183..185
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             186..203
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             204..248
                     /label=AviTag(TM)
                     /note="peptide tag that allows for enzymatic biotinylation"
     terminator      319..366
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      464..493
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      515..1102
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1114..1141)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1168..1356)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(1360..2172)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2173..2263)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(2264..2295)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
ORIGIN
        1 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
       61 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtag
      121 ggagaccaca acggtttccc tctagaaata attttgttta actataagaa ggagatatac
      181 atatgcatca tcaccaccat cacggtctga acgacatctt cgaggctcag aaaatcgaat
      241 ggcacgaaag cttatccacc ccgccgaccc cgagcactcc tcctacctaa tagagcggcc
      301 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      361 tttttgctga aaggaggaac tatatccggg taacgaattc aagcttgata tcattcagga
      421 cgagcctcag actccagcgt aactggactg caatcaactc actggctcac cttcacgggt
      481 gggcctttct tcggtagaaa atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
      541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      601 agctaccaac tctttttccg aggtaactgg cttcagcaga gcgcagatac caaatactgt
      661 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      901 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1021 ttatagtcct gtcgggtttc gccacctctg acttgagcat cgatttttgt gatgctcgtc
     1081 aggggggcgg agcctatgga aaaacgccag caacgcagaa aggcccaccc gaaggtgagc
     1141 caggtgatta catttgggcc ctcatcagag gttttcaccg tcatcaccga aacgcgcgag
     1201 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     1261 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     1321 ggccatgtta agggcggttt tttcctgttt ggtcatttag aaaaactcat cgagcatcaa
     1381 gtgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     1441 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     1501 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     1561 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     1621 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     1681 actcgcacca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     1741 atcgccgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     1801 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     1861 tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     1921 gatggtcgga agaggcataa attccgtcag ccagtttagc ctgaccatct catctgtaac
     1981 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     2041 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     2101 atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg
     2161 aatatggctc atagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     2221 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaagtcaaa agcctccggt
     2281 cggaggcttt tgactttctg ctatggaggt caggtatgat ttaaatggtc agtattgagc
     2341 gatatctaga gaattcgt
//
