LOCUS       40924_4514        5011 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative expression vector pAnFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Song DH, Li J, Hu XX, Xi B, Yang GL.
  TITLE     Integrative expression vector pAnFP, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Song DH, Li J, Hu XX, Xi B, Yang GL.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2013) Dept.of Marine Science, College of Marine 
REFERENCE   3  (bases 1 to 5011)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5011)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2013) Dept.of Marine Science, College of Marine Science "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    707..1221
                     /note="F-up flanking region; derived from Anabaena sp.
                     PCC7120"
     regulatory      1224..1388
                     /note="light inducible psbA1 promoter derived from Anabaena
                     sp. PCC7120"
                     /regulatory_class="promoter"
     CDS             1501..2313
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2321..2783
                     /note="F-dw flanking region; derived from Anabaena sp.
                     PCC7120"
     promoter        complement(2826..2844)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2865..2881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2889..2905)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2913..2943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2958..2979)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3267..3855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4029..4886)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4887..4991)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcacgc cataatcatg
      721 tgtcaaaaat tccttatata ttaatgaatt ttattttata aaacaatatt attgtttact
      781 ctacgcttta catagaaatt gtgaaaagct cttttccaga cgcttagaac ttagggacaa
      841 aaatgatttc tggatacttg cgctcaccgt gtagtacgtt tccagtggca attgttcagt
      901 ctgtagcaga tattcaaact gattgacttt acgactgaac agcttgatag gcttagatag
      961 tattgctatt aagtttcagt ttggtcacaa aaagtaacaa attaattaat cagtcagatt
     1021 tttattcctt tgttatcttt aaattttcgc atcaataaat aacacagata ggagttgact
     1081 ttgatttctg ttggcgcagt gtggcatagc tataggtact tgtgtatcta ggggatggtc
     1141 actccttcac caacaaaatt taaatgggat gccttatgga agtctaattg tatggttaga
     1201 gttgtaatta tccccactgc actgcaggga ttcccaaaga tagggggaat aattaacatt
     1261 aagaattatt aattcatggg tttttagtct agtaaatttg cgtgaattca tgtaaatttt
     1321 atgagacagg cgcaagtcta aaaaaagcgt ctgaattaat ctgcacaaat ccaaagcaat
     1381 cataaaaaca tatggggatc ccatcgattg acgctcagtg gaacgaaaac tcacgttaag
     1441 ggattttggt catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1501 atgagccata ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat
     1561 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1621 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1681 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1741 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1801 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1861 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1921 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1981 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2041 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2101 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2161 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2221 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2281 ttgcagtttc atttgatgct cgatgagttt ttctaaggat ccaattttga aacctatcaa
     2341 ctatattatt aaggcagtga agacttgcca taacctatat ttctcgcatg aagtaatctg
     2401 ctgagaacta tgagttgtgt aatatattgt atttttatta agtgtaggtt taattgcata
     2461 gagttagccg ttagaactaa ttatcccaga atagatacct caaattctca gtagcagaaa
     2521 ttaaatagat aacaaaatat acaaaatgcc ttcagtattt aaaacttctt ctcagaagac
     2581 aaacacagtt agtaaccaga tgaataactc atcaaaatca tcaacagaag tattagctgt
     2641 gtctcctgca tcccaaaggt atactatgca aaaattgcca tgtagagaca atattaataa
     2701 gttgtggaag ttatacagat caattttaag taaatgtatg tatacttctc tagaattagc
     2761 tgtgcattca cctatggtga gcctctagag cggccgccac cgcggtggag ctccagcttt
     2821 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     2881 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2941 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3001 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3061 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4981 ggggttccgc gcacatttcc ccgaaaagtg c
//
