LOCUS       40924_4424        3325 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing AmCyan in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             289..975
                     /codon_start=1
                     /label=AmCyan
                     /note="Anemonia majano cyan fluorescent protein"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKMTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHAVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     promoter        1420..1524
                     /label=AmpR promoter
     CDS             1525..2382
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2556..3144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggctctttca
      301 aacaagttta tcggagatga catgaaaatg acctaccata tggatggctg tgtcaatggg
      361 cattacttta ccgtcaaagg tgaaggcagc gggaagccat acgaagggac gcagacctcg
      421 acttttaaag tcaccatggc caacggtggg ccccttgcat tctcctttga catactatct
      481 acagtgttca tgtatggaaa tcgatgcttt actgcgtatc ctaccagtat gcccgactat
      541 ttcaaacaag catttcctga cggaatgtca tatgaaagga cttttaccta tgaagatgga
      601 ggagttgcta cagccagttg ggaaataagc cttaaaggca actgctttga gcacaaatcc
      661 acgtttcatg gagtgaactt tcctgctgat ggacctgtga tggcgaagat gacaactggt
      721 tgggacccat cttttgagaa aatgactgtc tgcgatggaa tattgaaggg tgatgtcacc
      781 gcgttcctca tgctgcaagg aggtggcaat tacagatgcc aattccacac ttcttacaag
      841 acaaaaaaac cggtgacgat gccaccaaac catgcggtgg aacatcgcat tgcgaggacc
      901 gaccttgaca aaggtggcaa cagtgttcag ctgacggagc acgctgttgc acatataacc
      961 tctgttgtcc ctttctgaag cggccgcgac tctagaattc caactgagcg ccggtcgcta
     1021 ccattaccaa cttgtctggt gtcaaaaata ataggcctac tagtcggccg tacgggccct
     1081 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1141 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1201 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     1261 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     1321 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1381 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1441 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1501 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1561 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1621 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1681 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1741 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1801 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1861 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1921 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1981 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2041 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2101 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2161 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2221 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2281 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2341 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2701 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3241 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3301 gagtcagtga gcgaggaagc ggaag
//
