LOCUS       V001189                 8175 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V001189
VERSION     V001189
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     205..354
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    complement(363..484)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(623..1339)
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        complement(1384..1587)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1588..1891)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             2081..2500
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2566..3900)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     repeat_region   5147..5249
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(5502..6090)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6903..7694
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     gap             8112..8175
                     /estimated_length=64
ORIGIN
        1 nnnttaatta annntccctt ccagctctct gccccttttg gattgaagcc aatatgataa
       61 tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag
      121 tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa
      181 aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta
      241 ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa
      301 actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaannncgc
      361 gttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
      421 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      481 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
      541 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      601 atccggtgga tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg
      661 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      721 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      781 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      841 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
      901 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      961 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     1021 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     1081 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     1141 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     1201 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     1261 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     1321 cctcgccctt gctcaccatg gtggcgaccg gtagcgctag cggatctgac ggttcactaa
     1381 accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     1441 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     1501 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     1561 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     1621 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     1681 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     1741 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     1801 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     1861 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     1921 tgaccccgta attgattact attannncta gcagatctgg taccgtcgac gcggccgcga
     1981 tatcctcgag aagctttcta gagnnntaag ggtgggaaag aatatataag gtgggggtct
     2041 tatgtagttt tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga
     2101 tggaagcatt gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca
     2161 gaatgtgatg ggctccagca ttgatggtcg ccccgtcctg cccgcaaact ctactacctt
     2221 gacctacgag accgtgtctg gaacgccgtt ggagactgca gcctccgccg ccgcttcagc
     2281 cgctgcagcc accgcccgcg ggattgtgac tgactttgct ttcctgagcc cgcttgcaag
     2341 cagtgcagct tcccgttcat ccgcccgcga tgacaagttg acggctcttt tggcacaatt
     2401 ggattctttg acccgggaac ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca
     2461 ggtttctgcc ctgaaggctt cctcccctcc caatgcggtt taaaacataa ataaaaaacc
     2521 agactctgtt tggatttgga tcaagcaagt gtcttgctgt ctttatttag gggttttgcg
     2581 cgcgcggtag gcccgggacc agcggtctcg gtcgttgagg gtcctgtgta ttttttccag
     2641 gacgtggtaa aggtgactct ggatgttcag atacatgggc ataagcccgt ctctggggtg
     2701 gaggtagcac cactgcagag cttcatgctg cggggtggtg ttgtagatga tccagtcgta
     2761 gcaggagcgc tgggcgtggt gcctaaaaat gtctttcagt agcaagctga ttgccagggg
     2821 caggcccttg gtgtaagtgt ttacaaagcg gttaagctgg gatgggtgca tacgtgggga
     2881 tatgagatgc atcttggact gtatttttag gttggctatg ttcccagcca tatccctccg
     2941 gggattcatg ttgtgcagaa ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc
     3001 atgtagctta gaaggaaatg cgtggaagaa cttggagacg cccttgtgac ctccaagatt
     3061 ttccatgcat tcgtccataa tgatggcaat gggcccacgg gcggcggcct gggcgaagat
     3121 atttctggga tcactaacgt catagttgtg ttccaggatg agatcgtcat aggccatttt
     3181 tacaaagcgc gggcggaggg tgccagactg cggtataatg gttccatccg gcccaggggc
     3241 gtagttaccc tcacagattt gcatttccca cgctttgagt tcagatgggg ggatcatgtc
     3301 tacctgcggg gcgatgaaga aaacggtttc cggggtaggg gagatcagct gggaagaaag
     3361 caggttcctg agcagctgcg acttaccgca gccggtgggc ccgtaaatca cacctattac
     3421 cgggtgcaac tggtagttaa gagagctgca gctgccgtca tccctgagca ggggggccac
     3481 ttcgttaagc atgtccctga ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc
     3541 gccgcccagc gatagcagtt cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc
     3601 cgccgtaggc atgcttttga gcgtttgacc aagcagttcc aggcggtccc acagctcggt
     3661 cacctgctct acggcatctc gatccagcat atctcctcgt ttcgcgggtt ggggcggctt
     3721 tcgctgtacg gcagtagtcg gtgctcgtcc agacgggcca gggtcatgtc tttccacggg
     3781 cgcagggtcc tcgtcagcgt agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg
     3841 ctggccaggg tgcgcttgag gctggtcctg ctggtgctga agcgctgccg gtcttcgccc
     3901 tgcgcgtcgg ccaggtagca tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg
     3961 cccttggcgc gcagcttgcc cttggaggag gcgccgcacg aggggcagtg cagacttttg
     4021 agggcgtaga gcttgggcgc gagaaatacc gattccgggg agtaggcatc cgcgccgcag
     4081 gccccgcaga cggtctcgca ttccacgagc caggtgagct ctggccgttc ggggtcaaaa
     4141 accaggtttc ccccatgctt tttgatgcgt ttcttacctc tggtttccat gagccggtgt
     4201 ccacgctcgg tgacgaaaag gctgtccgtg tccccgtata cagactnnng tttaaacgaa
     4261 ttcnnntata aaatgcaagg tgctgctcaa aaaatcaggc aaagcctcgc gcaaaaaaga
     4321 aagcacatcg tagtcatgct catgcagata aaggcaggta agctccggaa ccaccacaga
     4381 aaaagacacc atttttctct caaacatgtc tgcgggtttc tgcataaaca caaaataaaa
     4441 taacaaaaaa acatttaaac attagaagcc tgtcttacaa caggaaaaac aacccttata
     4501 agcataagac ggactacggc catgccggcg tgaccgtaaa aaaactggtc accgtgatta
     4561 aaaagcacca ccgacagctc ctcggtcatg tccggagtca taatgtaaga ctcggtaaac
     4621 acatcaggtt gattcatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca
     4681 tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg
     4741 agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg
     4801 ctccagaaca acatacagcg cttcacagcg gcagcctaac agtcagcctt accagtaaaa
     4861 aagaaaacct attaaaaaaa caccactcga cacggcacca gctcaatcag tcacagtgta
     4921 aaaaagggcc aagtgcagag cgagtatata taggactaaa aaatgacgta acggttaaag
     4981 tccacaaaaa acacccagaa aaccgcacgc gaacctacgc ccagaaacga aagccaaaaa
     5041 acccacaact tcctcaaatc gtcacttccg ttttcccacg ttacgtaact tcccatttta
     5101 agaaaactac aattcccaac acatacaagt tactccgccc taaaacctac gtcacccgcc
     5161 ccgttcccac gccccgcgcc acgtcacaaa ctccaccccc tcattatcat attggcttca
     5221 atccaaaata aggtatatta ttgatgatnn nttaattaag gatccnnncg gtgtgaaata
     5281 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     5341 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5941 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6541 ccagttaata gtttgcgcaa cgttgttgnn nnnnaaaaag gatcttcacc tagatccttt
     6601 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     6661 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     6721 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     6781 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc
     6841 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
     6901 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6961 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7021 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7081 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7141 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7201 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7261 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7321 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7381 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     7441 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7501 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7561 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7621 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7681 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa
     7741 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt
     7801 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     7861 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
     7921 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga
     7981 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     8041 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc
     8101 ttaatgcgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnn
//
