LOCUS       V014583                 9804 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014583
VERSION     V014583
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   79..181
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     CDS             491..874
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     CDS             1459..2793
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(2859..3278)
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     enhancer        3440..3743
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3744..3947
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    4158..4279
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        4669..4872
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             4917..6197
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     misc_signal     complement(6603..6753)
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   6841..6943
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      6981..7361
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(7412..8203)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      9035..9623
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgga
       61 tccatgcatg ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg
      121 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      181 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      241 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      301 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      361 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      421 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      481 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      541 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt
      601 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc
      661 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga
      721 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg
      781 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt
      841 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt
      901 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg
      961 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg
     1021 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa
     1081 caagctattg aattcgttta aactccctct caagtctgta tacggggaca cggacagcct
     1141 tttcgtcacc gagcgtggac accggctcat ggaaaccaga ggtaagaaac gcatcaaaaa
     1201 gcatggggga aacctggttt ttgaccccga acggccagag ctcacctggc tcgtggaatg
     1261 cgagaccgtc tgcggggcct gcggcgcgga tgcctactcc ccggaatcgg tatttctcgc
     1321 gcccaagctc tacgccctca aaagtctgca ctgcccctcg tgcggcgcct cctccaaggg
     1381 caagctgcgc gccaagggcc acgccgcgga ggggctggac tatgacacca tggtcaaatg
     1441 ctacctggcc gacgcgcagg gcgaagaccg gcagcgcttc agcaccagca ggaccagcct
     1501 caagcgcacc ctggccagcg cgcagcccgg agcgcacccc ttcaccgtga cccagactac
     1561 gctgacgagg accctgcgcc cgtggaaaga catgaccctg gcccgtctgg acgagcaccg
     1621 actactgccg tacagcgaaa gccgccccaa cccgcgaaac gaggagatat gctggatcga
     1681 gatgccgtag agcaggtgac cgagctgtgg gaccgcctgg aactgcttgg tcaaacgctc
     1741 aaaagcatgc ctacggcgga cggtctcaaa ccgttgaaaa actttgcttc cttgcaagaa
     1801 ctgctatcgc tgggcggcga gcgccttctg gcggatttgg tcagggaaaa catgcgagtc
     1861 agggacatgc ttaacgaagt ggcccccctg ctcagggatg acggcagctg cagctctctt
     1921 aactaccagt tgcagccggt aataggtgtg atttacgggc ccaccggctg cggtaagtcg
     1981 cagctgctca ggaacctgct ttcttcccag ctgatctccc ctaccccgga aaccgttttc
     2041 ttcatcgccc cgcaggtaga catgatcccc ccatctgaac tcaaagcgtg ggaaatgcaa
     2101 atctgtgagg gtaactacgc ccctgggccg gatggaacca ttataccgca gtctggcacc
     2161 ctccgcccgc gctttgtaaa aatggcctat gacgatctca tcctggaaca caactatgac
     2221 gttagtgatc ccagaaatat cttcgcccag gccgccgccc gtgggcccat tgccatcatt
     2281 atggacgaat gcatggaaaa tcttggaggt cacaagggcg tctccaagtt cttccacgca
     2341 tttccttcta agctacatga caaatttccc aagtgcaccg gatacactgt gctggtggtt
     2401 ctgcacaaca tgaatccccg gagggatatg gctgggaaca tagccaacct aaaaatacag
     2461 tccaagatgc atctcatatc cccacgtatg cacccatccc agcttaaccg ctttgtaaac
     2521 acttacacca agggcctgcc cctggcaatc agcttgctac tgaaagacat ttttaggcac
     2581 cacgcccagc gctcctgcta cgactggatc atctacaaca ccaccccgca gcatgaagct
     2641 ctgcagtggt gctacctcca ccccagagac gggcttatgc ccatgtatct gaacatccag
     2701 agtcaccttt accacgtcct ggaaaaaata cacaggaccc tcaacgaccg agaccgctgg
     2761 tcccgggcct accgcgcgcg caaaacccct aaataaagac agcaagacac ttgcttgatc
     2821 caaatccaaa cagagtctgg ttttttattt atgttttaaa ccgcattggg aggggaggaa
     2881 gccttcaggg cagaaacctg ctggcgcaga tccaacagct gctgagaaac gacattaagt
     2941 tcccgggtca aagaatccaa ttgtgccaaa agagccgtca acttgtcatc gcgggcggat
     3001 gaacgggaag ctgcactgct tgcaagcggg ctcaggaaag caaagtcagt cacaatcccg
     3061 cgggcggtgg ctgcagcggc tgaagcggcg gcggaggctg cagtctccaa cggcgttcca
     3121 gacacggtct cgtaggtcaa ggtagtagag tttgcgggca ggacggggcg accatcaatg
     3181 ctggagccca tcacattctg acgcaccccg gcccatgggg gcatgcgcgt tgtcaaatat
     3241 gagctcacaa tgcttccatc aaacgagttg gtgctcatgg cggcggcggc tgctgcaaaa
     3301 cagatacaaa actacataag acccccacct tatatattct ttcccaccct taaccacgcc
     3361 cagatcctct agcagtgata aacgtctaat agtaatcaat tacggggtca ttagttcata
     3421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3661 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3961 gtcagatccg ctagagatct ggtaccgtcg acgcggccgc tcgagcctaa gcttctagat
     4021 aagatatccg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     4081 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     4141 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4261 aactcatcaa tgtatcttaa cgcggaggtt tatcgacgat ctgctagtga ttaatagtaa
     4321 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     4381 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     4441 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     4501 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4561 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     4621 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     4681 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     4741 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     4801 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     4861 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgt
     4921 cgaataacgc tttacaaaca attattaacg cccggttacc aggcgaagag gggctgtggc
     4981 agattcatct gcaggacgga aaaatcagcg ccattgatgc gcaatccggc gtgatgccca
     5041 taactgaaaa cagcctggat gccgaacaag gtttagttat accgccgttt gtggagccac
     5101 atattcacct ggacaccacg caaaccgccg gacaaccgaa ctggaatcag tccggcacgc
     5161 tgtttgaagg cattgaacgc tgggccgagc gcaaagcgtt attaacccat gacgatgtga
     5221 aacaacgcgc atggcaaacg ctgaaatggc agattgccaa cggcattcag catgtgcgta
     5281 cccatgtcga tgtttcggat gcaacgctaa ctgcgctgaa agcaatgctg gaagtgaagc
     5341 aggaagtcgc gccgtggatt gatctgcaaa tcgtcgcctt ccctcaggaa gggattttgt
     5401 cgtatcccaa cggtgaagcg ttgctggaag aggcgttacg cttaggggca gatgtagtgg
     5461 gggcgattcc gcattttgaa tttacccgtg aatacggcgt ggagtcgctg cataaaacct
     5521 tcgccctggc gcaaaaatac gaccgtctca tcgacgttca ctgtgatgag atcgatgacg
     5581 agcagtcgcg ctttgtcgaa accgttgctg ccctggcgca ccatgaaggc atgggcgcgc
     5641 gagtcaccgc cagccacacc acggcaatgc actcctataa cggggcgtat acctcacgcc
     5701 tgttccgctt gctgaaaatg tccggtatta actttgtcgc caacccgctg gtcaatattc
     5761 atctgcaagg acgtttcgat acgtatccaa aacgtcgcgg catcacgcgc gttaaagaga
     5821 tgctggagtc cggcattaac gtctgctttg gtcacgatga tgtcttcgat ccgtggtatc
     5881 cgctgggaac ggcgaatatg ctgcaagtgc tgcatatggg gctgcatgtt tgccagttga
     5941 tgggctacgg gcagattaac gatggcctga atttaatcac ccaccacagc gcaaggacgt
     6001 tgaatttgca ggattacggc attgccgccg gaaacagcgc caacctgatt atcctgccgg
     6061 ctgaaaatgg gtttgatgcg ctgcgccgtc aggttccggt acgttattcg gtacgtggcg
     6121 gcaaggtgat tgccagcaca caaccggcac aaaccaccgt atatctggag cagccagaag
     6181 ccatcgatta caaacgttga tccggactca gatccaccgg atctagataa ctgatcataa
     6241 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6301 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6361 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6421 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgatcaa gctagcttgc
     6481 tagactcgac tgactataat aataaaacgc caactttgac ccggaacgcg gaaaacacct
     6541 gagaaaaaca cctgggcgag tctccacgta aacggtcaaa gtccccgcgg ccctagacaa
     6601 atattacgcg ctatgagtaa cacaaaatta ttcagatttc acttcctctt attcagtttt
     6661 cccgcgaaaa tggccaaatc ttactcggtt acgcccaaat ttactacaac atccgcctaa
     6721 aaccgcgcga aaattgtcac ttcctgtgta caccggcgca caccaaaaac gtcacttttg
     6781 ccacatccgt cgcttacatg tgttccgcca cacttgcaac atcacacttc cgccacacta
     6841 ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac cccctcatta
     6901 tcatattggc ttcaatccaa aataaggtat attattgatg atgttaatta agaattaatt
     6961 cgatcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7021 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7081 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7141 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7201 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7261 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7321 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7381 gctgatttaa caaaaatttt aacaaaattc agaagaactc gtcaagaagg cgatagaagg
     7441 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     7501 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     7561 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     7621 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct
     7681 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     7741 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     7801 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     7861 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     7921 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     7981 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
     8041 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     8101 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     8161 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     8221 ctgtctcttg atcagagctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     8281 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     8341 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
     8401 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     8461 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgaaaagg atctaggtga
     8521 agatcctttt tgataatctc atggctgcag caatggcaac aacgttgcgc aaactattaa
     8581 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8641 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8701 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8761 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8821 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9181 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9781 agtcagtgag cgaggaagcg gaag
//
