LOCUS       40924_4036        5342 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pAdD26SV(A)-3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5342)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5342)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..1242
                     /label=TcR
                     /note="tetracycline efflux protein"
     rep_origin      complement(1450..2038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(3069..3203)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3393..3953)
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     intron          complement(4089..4318)
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     regulatory      complement(4318..5101)
                     /label=Adenovirus 2 major late promoter
                     /note="Adenovirus 2 major late promoter"
                     /regulatory_class="promoter"
     promoter        5121..5331
                     /label=SV40 promoter
                     /note="SV40 early promoter"
ORIGIN
        1 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
       61 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
      121 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc
      181 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt
      241 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
      301 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga
      361 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
      421 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
      481 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
      541 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
      601 gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
      661 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
      721 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
      781 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
      841 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
      901 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
      961 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     1021 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     1081 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
     1141 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     1201 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     1261 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     1321 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     1381 gcacgcggcg catctcggga aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1861 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     1921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2521 tgctgcaggg tgtgttagca aactacagga ccaaatatcc tgctcaaact gtaaccccaa
     2581 aaaatgctac agttgacagt cagcagatga acactgacca caaggctgtt ttggataagg
     2641 ataatgctta tccagtggag tgctgggttc ctgatccaag taaaaatgaa aacactagat
     2701 attttggaac ctacacaggt ggggaaaatg tgcctcctgt tttgcacatt actaacacag
     2761 caaccacagt gcttcttgat gagcagggtg ttgggccctt gtgcaaagct gacagcttgt
     2821 atgtttctgc tgttgacatt tgtgggctgt ttaccaacac ttctggaaca cagcagtgga
     2881 agggacttcc cagatatttt aaaattaccc ttagaaagcg gtctgtgaaa aacccctacc
     2941 caatttcctt tttgttaagt gacctaatta acaggaggac acagagggtg gatgggcagc
     3001 ctatgattgg aatgtcctct caagtagagg aggttagggt ttatgaggac acagaggagc
     3061 ttcctgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3121 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3181 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3241 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     3301 attatgatct aaagccagca aaagtcccat ggtcttataa aaatgcatag ctttaggagg
     3361 ggagcagaga acttgaaagc atcttcctgt tagtctttct tctcgtagac ttcaaactta
     3421 tacttgatgc ctttttcctc ctggacctca gagaggacgc ctgggtattc tgggagaagt
     3481 ttatatttcc ccaaatcaat ttctgggaaa aacgtgtcac tttcaaattc ctgcatgatc
     3541 cttgtcacaa agagtctaag gtggcctggt tgattcatgg cttcctggta aacagaactg
     3601 cctccgacta tccaaaccat gtctacttta cttgccaatt ccggttgttc aataagtctt
     3661 aaggcatcat ccaaactttt ggcaagaaaa tgagctcctc gtggtggttc tttgagttct
     3721 ctactgagaa ctatattaat tctgtccttt aaaggtcgat tcttctcagg aatggagaac
     3781 caggttttcc tacccataat caccagattc tgtttacctt ccactgaaga ggttgtggtc
     3841 attctttgga agtacttgaa ctcgttcctg agcggaggcc agggtaggtc tccgttcttg
     3901 ccaatcccca tattttggga cacggcgacg atgcagttca atggtcgaac catgatggca
     3961 gcggggataa aatcctacca gccttcacgc taggattgcc gtcaagtttg gcgcgaaatc
     4021 gcagccctga gctgtgtacc acttnnnccc cccccccccc ccctgcagtt ggacctggga
     4081 gtggacacct gtggagagaa aggcaaagtg gatgtcattg tcactcaagt gtatggccag
     4141 atctcaagcc tgccacacct caagcttgac aacaaaaaga ttgtcttttc tgaccagatg
     4201 gacgcggcca ccctcaaagg catcaccgcg ggccaggtga atatcaaatc ctcctcgttt
     4261 ttggaaactg acaatcttag cgcagaagtc atgcccgctt ttgagaggga gtactcaccc
     4321 caacagctgg ccctcgcaga cagcgatgcg gaagagagtg aggacgaacg cgcccccacc
     4381 cccttttata gccccccttc aggaacaccc ggtcacgtgg cctacaccta taaaccaatc
     4441 accttccttg atgccgaaga gggcgacatg tgtcttcaca ccctggagcg agtggacccc
     4501 ctagtggaca acgaccgcta cccctcccac ttagcctcct tcgtgctggc ctggacgcga
     4561 gccttcgtct cagagtggtc cgagtttcta tacgaggagg accgcggaac accgctcgag
     4621 gacaggcctc tcaagtctgt atacggggac acggacagcc ttttcgtcac cgagcgtgga
     4681 caccggctca tggaaaccag aggtaagaaa cgcatcaaaa agcatggggg aaacctggtt
     4741 tttgaccccg aacggccaga gctcacctgg ctcgtggaat gcgagaccgt ctgcggggcc
     4801 tgcggcgcgg atgcctactc cccggaatcg gtatttctcg cgcccaagct ctacgccctt
     4861 aaaagtctgc actgcccctc gtgcggcgcc tcctccaagg gcaagctgcg cgccaagggc
     4921 cacgccgcgg aggggctgga ctatgacacc atggtcaaat gctacctggc cgacgcgcag
     4981 ggcgaagacc ggcagcgctt cagcaccagc aggaccagcc tcaagcgcac cctggccagc
     5041 gcgcagcccg gagcgcaccc cttcaccgtg acccagacta cgctgacgag gaccctgcgc
     5101 cnnngngaat tcnctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5161 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5221 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5281 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5341 ag
//
