LOCUS       40924_3916        9418 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAct26IFN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Levraud JP, Boudinot P, Colin I, Benmansour A, Peyrieras N, Herbomel
            P, Lutfalla G.
  TITLE     Identification of the zebrafish IFN receptor: implications for the 
            origin of the vertebrate IFN system
  JOURNAL   J. Immunol. 178 (7), 4385-4394 (2007)
  PUBMED    17371995
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Lutfalla G.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2006) UMR5124, CNRS/UMII, place Eugene Bataillon, 
            Montpellier cedex 5 34095, France
REFERENCE   3  (bases 1 to 9418)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9418)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Immunol."; date: "2007"; volume: "178"; issue: "7"; pages: 
            "4385-4394"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-SEP-2006) UMR5124, CNRS/UMII, place Eugene Bataillon, 
            Montpellier cedex 5 34095, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5595..6140
                     /codon_start=1
                     /product="IFN-lambda"
                     /label=IFN-lambda
                     /note="helical cytokine"
                     /protein_id="ABJ97321.1"
                     /translation="MWTYIFVIYVILQSQSSASTCEWLGRYRIITTESLNLLKNMGGKY
                     ADLETPFPSRLYTLMDKSKVEDQVKFLVLTLDHIIHLMDAREHMNSVNWDQNTVEDFLN
                     ILHRKSSDLKECVARYAKPAHKESYEIRIKRHFRTLKKILKKKQYSAEAWEQIRRVVKS
                     HLQRMDIIASNARVNPRV"
     CDS             6158..6844
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTG"
     polyA_signal    7059..7180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7227..7245)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7266..7282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7290..7306)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7314..7344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7359..7380)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7668..8256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8430..9287)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9288..9392)
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtactaggg
      661 ataacagggt aatctcgagc ggccgccagt gtgatggata tctgcagaat tcgccctttc
      721 gactgggtta gttggcattt ctgtgtggac tttgtatgct ctccctgtgt ttgagtgggt
      781 ttcctccggg tgctccagta tcccccacag tccaaagaca tgcgtataag tgtccgtagt
      841 gaatgagtgt gtgttttcca gtgatgggat gcagctggaa gggaatctgc tgcgtaaaac
      901 atatgctgaa taagttggtg gttcattccg cagtggcgac cccagattaa caaagggact
      961 gagccaaaaa gaaaaggaat aaatgaatga tctctataaa ttaatctatg tatgttttca
     1021 atcaaataac tgaattttca ttaagggaag ctgttaaaat ggagaatctc ctttacataa
     1081 tttaactgtg tgcgaaaaga gaaactgaat ctttaaatgc ctgagtttga gcatgttctg
     1141 acacacttac attcctggaa tgacagccac taaatactgc cttcctctcc tttttagcaa
     1201 agatgcgttt gtaaccctat acattgaatt caattgcagg gtgcaatatt tctttcttga
     1261 cttctccttg gttttattta catttatacc ttcttaaaat acattttggc atcactggtt
     1321 ccatgaagaa cctttaaaag aggctttcca ttgaacagaa agttctttat cagtgtgaaa
     1381 aagctctgta gatgatgtaa aatgctcttg acaaaaaatc ctatgtatat ataactgttt
     1441 gctgaaggat tctttagaga aacaaacacg gttcttctat ggcatttaga acctttcaga
     1501 gtgcaacagc ctaattatac tacataaagt tggcttcttc cacatatctt aactaaatgc
     1561 aaagcactga gctctgacat gccctcaaag taagcaactg acacattgcc agccaataaa
     1621 aatacctgca atgtttgtac ttgtggaaaa catattaaaa taattgcacc aaagtaaact
     1681 aatggtgttt ttttaggcat cgatgattcc atcaagaaca tttgaaaccc acagaacatt
     1741 tccattgcac gagatgtacg ttataggggg aagaaacgct gagaaaaagt ttattttcag
     1801 tagaaaaatg ttatttttct tctataaacc acttgttgaa acattttttc aatgtgtaaa
     1861 tgttgtgtac catattacat cagtcactct tagttcccct ctgtcaatct cattttggtt
     1921 acatttccat attaggaatc tacctgtatc agtgcttaga gcgagcagcc tcaaccagtc
     1981 catcccatca gaactctgag tttgttacct gttggtgttt ccatataggg tgctccaggt
     2041 ctgatgggtg gagacagtca gtaggccact tctctgtgta atattattgt gttctttcat
     2101 tctcctatgc tgtatacagt aaacaacaga gcagatgttc agcaaattaa aaaaacgaca
     2161 acatttaata aaagcaacaa caagagagaa tcgtagactc tcaatcactc tgtctaattt
     2221 ccgctgtaag tcacatgact tcgccttggt tgcaaagaac tacaccatgt gactcatcca
     2281 cagattgcct tataaggaga ttggctgaat atctcttaaa ggggccctga acaaagtgtc
     2341 tcagcaaaca agacacaaca caaaggttat ctcatttcct tcatattttt cattgaccta
     2401 tggctttgga agaaaatcat ttttagttgc tgtgtgtaca tatgcaagct cccatcacaa
     2461 taaaacacac ccatatgtca aaacagctgc tcagactaat aaacgaacaa gcaatcatgc
     2521 tttacaagtt taccacagag tttatatgct agacttattt ccacagggct cctaaaatta
     2581 gctttgctca ctttatattt gttttttgat agaagcctca gcaacttcct ctcagatttg
     2641 tcacattgtg ctgtggctgc tgtaacactt agtcgcctct ctctctcttc ctcttcctct
     2701 ttctgtttga aacaacctta taagggcctc agtaatctgc tgatgacaga aatgatagga
     2761 aatcaaaaag ttatcataaa cacatatgca atactttcta gtaatcataa aagcacagta
     2821 cattcaaaaa tgacgtattt ctcactattt actcatcctc aagtggttaa aaaccttaaa
     2881 gagtttcatt tttctgttca aaacacagga gatattttga ggaaagctaa aaacctgtag
     2941 cctaaccatt gacttgcata gtaggaaaaa aatagaaacc ttttcagatt tcctctacag
     3001 ctaaaaagaa actcccctcc accctaaaca ccaagctgaa actcacacac ctaggaaaga
     3061 atgggcccgg tatcaggaat gtagagcact ttgtgaaata atcatgaggc aataaactgt
     3121 acctatgact cattgacctc tgtgatgaag tttatctttt ctataaggct cagtgagtta
     3181 taggtatgac atttggattc tataggtagt caaaattcaa tatttacatc actgcacgcc
     3241 taggatgtgg gggtgggcag atgtattggg tgtcactgct gttggaagca tcattacaga
     3301 cattttctga tgacaagctg ctttcgtcac tagataaatc acacaacaga ctgtttatca
     3361 agtggtgaca tagccctact gacatttcaa taaccatagc tcctaaagat tttaacatta
     3421 aatgcacaat ttagtcctct ttttggtttc atgctgacaa gataagacaa gaattgcaaa
     3481 gccccctgga taaggcatgc tgccacgcgt ctcagattgc ccatgctaaa ggcaggacat
     3541 tataactggc attaacatct atttatgacc ccatggcctc ctaaaaaacc cgaataggta
     3601 aaggatttac atatttcaat gtaaattcat atcaaatcga tctgggacct tgtttagcca
     3661 aaataaacgc tgtgtagtat tttggtttct ttcatgatag catttgttgc taatagtccc
     3721 tacacattta tcaatgtaaa ctagtctttg cttcaaaaca tcagcattta acatttaaga
     3781 atgggcagat tagggagtgg gcagatgtat taggtgtcat cctggtgacg ttttaacagc
     3841 cattgctcct tacgatttta acattaaatg cacaatttag tccccttttt ggtttcatgc
     3901 tgataagata agaattgcaa agcaccatgg atagggcgtg ctgccacgcg tctcagattg
     3961 cccaagctga aggcaggaca tttaaaactg ggattaacac ctatttatga ccccttggtt
     4021 cctctgaaaa accggaatat gctaaagaat ttacatattt tcaatgaaaa gtcatattaa
     4081 atcaatttgg gatctcgttt agcctaaata ggaacccttt ctagtatttt tttttccttg
     4141 aagccattag cctagcctat cagtgtcaca ttactttatc gaatgtaaac taaacagtct
     4201 gtgcttcaaa acattcacga catatagtgt tattcaatag tttatattat cagtcttttt
     4261 aagtaacagg gtatgtaggc tgattttttt tgtgcactct tcagattcca gtaatgcagc
     4321 agcgtgaagc gtcatcggtt tatgacgtaa ttacgcaaaa gccggcttga caggaagtgt
     4381 tgttttctat ggagcaccgt gcccttgttt tgtatcccct gctttgcaga taaccagata
     4441 aaatgaaata tgcttaatac ggttgcatct aacatgccga gcggcgatgt aacagacgcc
     4501 gatctgagca acaacaaaac aatagtcaat atttcattaa tttccgaatg actcctgatc
     4561 ttctgcagct ttacatccat gatattataa acagcaaagc attatgcaca aaagcaacct
     4621 acgattgaat taaacagtgc aatgttcaca aataaagcct tttttaaata tgcttttaca
     4681 ttatgtttta tagagtcacg agaagtacct gtactttaaa tgggagccac gcccacgttg
     4741 ttcatcaaac taacctaata aaatgacaca cttaaaatgt cttctcaaga attaatcgta
     4801 aatgagtcgt tttcaactag ctgtcaaaca ttaactgaaa tgtaaagcag attagtgtat
     4861 aacgttaatc aaataatgat ttcttctctg ttctaccaat aatggctctt gtataacgtt
     4921 agtttacaca ttgttgtcat ttatagagct gaaacataca gtaaaataca atataagttt
     4981 aaatactatt ggacaatgtc gtactgctat gtttaaacgt cctgtcattc ctgcagccta
     5041 agtacagctt atataaatgc aactttacat catgcattgc tcgtttttca cagaaataat
     5101 aaactattta acgttactcg ctgtatgtga ggttatttaa aagtaacgtt aagccacaga
     5161 catgaagtct agcatccttt actgatttaa ctgtgttcca atccaaatga tatacatatc
     5221 cttaattcaa gtgttacatc tgccaatgct gtgtcaaatc aacttatcgt gtatttcctt
     5281 atcaaattat ttcgcctttt cgccgatccc tgacatacat tatttcaacc cagaacttga
     5341 atttcaagca actagcttga aactcgccaa gtggacgacg gctggccaat cagaccgctc
     5401 gctgccgaaa gtttacctta tatggaagga gccggccgca gtgccggcta taaagctgtg
     5461 acccacctca cgctcagcat tgtgagtttt cagtgcacgc tgagaagatc ttcactcaag
     5521 ggcgaattcc agcacactgg cggccgttac tagtggatcc agaattctta atacacgcaa
     5581 agatgagaac tcaaatgtgg acctatattt ttgtgatata tgttattctg cagagtcaaa
     5641 gctctgcgtc tacttgcgaa tggcttggcc gatacaggat aataacgaca gaatctctga
     5701 acctgctcaa gaatatgggt ggaaaatatg cagatctgga gacaccattt ccaagccgct
     5761 tgtacacctt gatggacaag tcaaaggtgg aggaccaggt gaagttttta gtcctgacat
     5821 tggatcacat catccacctt atggatgcca gggagcacat gaactcggtg aactgggacc
     5881 aaaacactgt tgaagacttt cttaacatac tgcacagaaa atcgtctgac ctcaaagaat
     5941 gtgtggcaag atacgcaaag ccagcacaca aggagtccta cgagataaga ataaaaaggc
     6001 acttcaggac tttgaagaag attctaaaga aaaaacaata cagtgctgaa gcatgggagc
     6061 agatccggag agttgtgaaa agccaccttc agaggatgga catcatcgca agcaacgcaa
     6121 gggtcaatcc tcgagtgtaa gaattcagga tcccaccatg gtgagcaagg gcgaggagga
     6181 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     6241 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     6301 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     6361 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     6421 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     6481 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     6541 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     6601 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     6661 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     6721 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     6781 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     6841 cggcgcctcc ggactcagat ctaagctgaa ccctcctgat gagagtggcc ccggctgcat
     6901 gagctgcaag tgtgtgctct cctgaggatc gatccaccgg atctagataa ctgatcataa
     6961 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     7021 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     7081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7141 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgttaag attaccctgt
     7201 tatccctaga gctccagctt ttgttccctt tagtgagggt taattccgag cttggcgtaa
     7261 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7321 ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta
     7381 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7441 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7681 ggcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7741 ggactataaa gataccaggc gttcccccct ggaagctccc tcgtgcgctc tcctgttccg
     7801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7861 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8101 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8221 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8281 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8341 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8401 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8461 gcgatctgtc tatttcgttc atccatagtt gcctgactgc ccgtcgtgta gataactacg
     8521 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8581 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8641 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8701 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8761 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8821 tgatccccca tgttgtgaaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8881 agtaagttgg ccgcagtgtt atcactcatg cttatggcag cactgcataa ttctcttact
     8941 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9001 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9061 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9121 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9181 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9241 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9301 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9361 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
