LOCUS       40924_3911       11474 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAct26CRFB6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Levraud JP, Boudinot P, Colin I, Benmansour A, Peyrieras N, Herbomel
            P, Lutfalla G.
  TITLE     Identification of the zebrafish IFN receptor: implications for the 
            origin of the vertebrate IFN system
  JOURNAL   J. Immunol. 178 (7), 4385-4394 (2007)
  PUBMED    17371995
REFERENCE   2  (bases 1 to 11474)
  AUTHORS   Lutfalla G.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2006) UMR5124, CNRS/UMII, place Eugene Bataillon, 
            Montpellier cedex 5 34095, France
REFERENCE   3  (bases 1 to 11474)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Immunol."; date: "2007"; volume: "178"; issue: "7"; pages: 
            "4385-4394"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-SEP-2006) UMR5124, CNRS/UMII, place Eugene Bataillon, 
            Montpellier cedex 5 34095, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5827..6777
                     /codon_start=1
                     /product="zebrafish CRFB6 receptor"
                     /label=zebrafish CRFB6 receptor
                     /note="helical cytokine receptor; transmembrane"
                     /protein_id="ABJ97330.1"
                     /translation="MTYIIHLTVFMLIVNIERTCCLNPPQDLKIVKSQLLWKPPEVDGD
                     LRYSLQYKLDSKAEDKWYNVSSHINKNVFNITDEFYGALFRVRAEKGYHISEWAISNRV
                     NCVNVNSCAPVVNLSEKPGIVSLTLAHMDQSLEKEHGEHLEFNISSWSVNSRENPEEDV
                     VINSKDYIFNDLESGQIYCFQVEYLLYHKPYGKASKERCVFIPETPEAKKKRVLIYGPL
                     ITCFVLMVCGFCIFLFCKCKSNKRVQSVVKEWFQPFKLDLPDHYKEFLSSEFPVGLAPS
                     PSIPSLQSDDFIILKENADVEENGQEAEQQKTEIL"
     primer_bind     complement(8185..8201)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8229..8903
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     polyA_signal    9115..9236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(9283..9301)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9322..9338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9346..9362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9370..9400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9415..9436)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9724..10312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10486..11343)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11344..11448)
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtactaggg
      661 ataacagggt aatctcgagc ggccgccagt gtgatggata tctgcagaat tcgccctttc
      721 gactgggtta gttggcattt ctgtgtggac tttgtatgct ctccctgtgt ttgagtgggt
      781 ttcctccggg tgctccagta tcccccacag tccaaagaca tgcgtataag tgtccgtagt
      841 gaatgagtgt gtgttttcca gtgatgggat gcagctggaa gggaatctgc tgcgtaaaac
      901 atatgctgaa taagttggtg gttcattccg cagtggcgac cccagattaa caaagggact
      961 gagccaaaaa gaaaaggaat aaatgaatga tctctataaa ttaatctatg tatgttttca
     1021 atcaaataac tgaattttca ttaagggaag ctgttaaaat ggagaatctc ctttacataa
     1081 tttaactgtg tgcgaaaaga gaaactgaat ctttaaatgc ctgagtttga gcatgttctg
     1141 acacacttac attcctggaa tgacagccac taaatactgc cttcctctcc tttttagcaa
     1201 agatgcgttt gtaaccctat acattgaatt caattgcagg gtgcaatatt tctttcttga
     1261 cttctccttg gttttattta catttatacc ttcttaaaat acattttggc atcactggtt
     1321 ccatgaagaa cctttaaaag aggctttcca ttgaacagaa agttctttat cagtgtgaaa
     1381 aagctctgta gatgatgtaa aatgctcttg acaaaaaatc ctatgtatat ataactgttt
     1441 gctgaaggat tctttagaga aacaaacacg gttcttctat ggcatttaga acctttcaga
     1501 gtgcaacagc ctaattatac tacataaagt tggcttcttc cacatatctt aactaaatgc
     1561 aaagcactga gctctgacat gccctcaaag taagcaactg acacattgcc agccaataaa
     1621 aatacctgca atgtttgtac ttgtggaaaa catattaaaa taattgcacc aaagtaaact
     1681 aatggtgttt ttttaggcat cgatgattcc atcaagaaca tttgaaaccc acagaacatt
     1741 tccattgcac gagatgtacg ttataggggg aagaaacgct gagaaaaagt ttattttcag
     1801 tagaaaaatg ttatttttct tctataaacc acttgttgaa acattttttc aatgtgtaaa
     1861 tgttgtgtac catattacat cagtcactct tagttcccct ctgtcaatct cattttggtt
     1921 acatttccat attaggaatc tacctgtatc agtgcttaga gcgagcagcc tcaaccagtc
     1981 catcccatca gaactctgag tttgttacct gttggtgttt ccatataggg tgctccaggt
     2041 ctgatgggtg gagacagtca gtaggccact tctctgtgta atattattgt gttctttcat
     2101 tctcctatgc tgtatacagt aaacaacaga gcagatgttc agcaaattaa aaaaacgaca
     2161 acatttaata aaagcaacaa caagagagaa tcgtagactc tcaatcactc tgtctaattt
     2221 ccgctgtaag tcacatgact tcgccttggt tgcaaagaac tacaccatgt gactcatcca
     2281 cagattgcct tataaggaga ttggctgaat atctcttaaa ggggccctga acaaagtgtc
     2341 tcagcaaaca agacacaaca caaaggttat ctcatttcct tcatattttt cattgaccta
     2401 tggctttgga agaaaatcat ttttagttgc tgtgtgtaca tatgcaagct cccatcacaa
     2461 taaaacacac ccatatgtca aaacagctgc tcagactaat aaacgaacaa gcaatcatgc
     2521 tttacaagtt taccacagag tttatatgct agacttattt ccacagggct cctaaaatta
     2581 gctttgctca ctttatattt gttttttgat agaagcctca gcaacttcct ctcagatttg
     2641 tcacattgtg ctgtggctgc tgtaacactt agtcgcctct ctctctcttc ctcttcctct
     2701 ttctgtttga aacaacctta taagggcctc agtaatctgc tgatgacaga aatgatagga
     2761 aatcaaaaag ttatcataaa cacatatgca atactttcta gtaatcataa aagcacagta
     2821 cattcaaaaa tgacgtattt ctcactattt actcatcctc aagtggttaa aaaccttaaa
     2881 gagtttcatt tttctgttca aaacacagga gatattttga ggaaagctaa aaacctgtag
     2941 cctaaccatt gacttgcata gtaggaaaaa aatagaaacc ttttcagatt tcctctacag
     3001 ctaaaaagaa actcccctcc accctaaaca ccaagctgaa actcacacac ctaggaaaga
     3061 atgggcccgg tatcaggaat gtagagcact ttgtgaaata atcatgaggc aataaactgt
     3121 acctatgact cattgacctc tgtgatgaag tttatctttt ctataaggct cagtgagtta
     3181 taggtatgac atttggattc tataggtagt caaaattcaa tatttacatc actgcacgcc
     3241 taggatgtgg gggtgggcag atgtattggg tgtcactgct gttggaagca tcattacaga
     3301 cattttctga tgacaagctg ctttcgtcac tagataaatc acacaacaga ctgtttatca
     3361 agtggtgaca tagccctact gacatttcaa taaccatagc tcctaaagat tttaacatta
     3421 aatgcacaat ttagtcctct ttttggtttc atgctgacaa gataagacaa gaattgcaaa
     3481 gccccctgga taaggcatgc tgccacgcgt ctcagattgc ccatgctaaa ggcaggacat
     3541 tataactggc attaacatct atttatgacc ccatggcctc ctaaaaaacc cgaataggta
     3601 aaggatttac atatttcaat gtaaattcat atcaaatcga tctgggacct tgtttagcca
     3661 aaataaacgc tgtgtagtat tttggtttct ttcatgatag catttgttgc taatagtccc
     3721 tacacattta tcaatgtaaa ctagtctttg cttcaaaaca tcagcattta acatttaaga
     3781 atgggcagat tagggagtgg gcagatgtat taggtgtcat cctggtgacg ttttaacagc
     3841 cattgctcct tacgatttta acattaaatg cacaatttag tccccttttt ggtttcatgc
     3901 tgataagata agaattgcaa agcaccatgg atagggcgtg ctgccacgcg tctcagattg
     3961 cccaagctga aggcaggaca tttaaaactg ggattaacac ctatttatga ccccttggtt
     4021 cctctgaaaa accggaatat gctaaagaat ttacatattt tcaatgaaaa gtcatattaa
     4081 atcaatttgg gatctcgttt agcctaaata ggaacccttt ctagtatttt tttttccttg
     4141 aagccattag cctagcctat cagtgtcaca ttactttatc gaatgtaaac taaacagtct
     4201 gtgcttcaaa acattcacga catatagtgt tattcaatag tttatattat cagtcttttt
     4261 aagtaacagg gtatgtaggc tgattttttt tgtgcactct tcagattcca gtaatgcagc
     4321 agcgtgaagc gtcatcggtt tatgacgtaa ttacgcaaaa gccggcttga caggaagtgt
     4381 tgttttctat ggagcaccgt gcccttgttt tgtatcccct gctttgcaga taaccagata
     4441 aaatgaaata tgcttaatac ggttgcatct aacatgccga gcggcgatgt aacagacgcc
     4501 gatctgagca acaacaaaac aatagtcaat atttcattaa tttccgaatg actcctgatc
     4561 ttctgcagct ttacatccat gatattataa acagcaaagc attatgcaca aaagcaacct
     4621 acgattgaat taaacagtgc aatgttcaca aataaagcct tttttaaata tgcttttaca
     4681 ttatgtttta tagagtcacg agaagtacct gtactttaaa tgggagccac gcccacgttg
     4741 ttcatcaaac taacctaata aaatgacaca cttaaaatgt cttctcaaga attaatcgta
     4801 aatgagtcgt tttcaactag ctgtcaaaca ttaactgaaa tgtaaagcag attagtgtat
     4861 aacgttaatc aaataatgat ttcttctctg ttctaccaat aatggctctt gtataacgtt
     4921 agtttacaca ttgttgtcat ttatagagct gaaacataca gtaaaataca atataagttt
     4981 aaatactatt ggacaatgtc gtactgctat gtttaaacgt cctgtcattc ctgcagccta
     5041 agtacagctt atataaatgc aactttacat catgcattgc tcgtttttca cagaaataat
     5101 aaactattta acgttactcg ctgtatgtga ggttatttaa aagtaacgtt aagccacaga
     5161 catgaagtct agcatccttt actgatttaa ctgtgttcca atccaaatga tatacatatc
     5221 cttaattcaa gtgttacatc tgccaatgct gtgtcaaatc aacttatcgt gtatttcctt
     5281 atcaaattat ttcgcctttt cgccgatccc tgacatacat tatttcaacc cagaacttga
     5341 atttcaagca actagcttga aactcgccaa gtggacgacg gctggccaat cagaccgctc
     5401 gctgccgaaa gtttacctta tatggaagga gccggccgca gtgccggcta taaagctgtg
     5461 acccacctca cgctcagcat tgtgagtttt cagtgcacgc tgagaagatc ttcactcaag
     5521 ggcgaattcc agcacactgg cggccgttac tagtggatcg ggactggaat gcaacgcttg
     5581 ccgatgggat ttaaacgtaa aggaatgcaa agaaaacatt tgtttctcgc atgttgggtt
     5641 taaataatgt ttttgtgttt tactgatata gcacgtgtcg cttatacaga attcagcgca
     5701 tttagcaaca cttgctctgt ttatcgtaag agggacaggc gtatttcctg aagaaatcag
     5761 caagaacgac gctttctcaa ctctcgcaaa tggtttggtg aaagatgtgg atttatattt
     5821 gtcgaaatga cctatataat tcacctaacg gttttcatgt tgatcgtcaa tatcgagaga
     5881 acatgctgct taaatccgcc tcaggattta aagatagtca aatcacagtt gctgtggaag
     5941 ccacctgaag tagatggaga tctgcggtat tcccttcagt ataaacttga ctccaaagca
     6001 gaggataaat ggtacaacgt gtcaagccat atcaacaaaa acgttttcaa catcactgat
     6061 gaattttatg gagctttatt tcgggtccgt gcagagaagg gataccacat ctcagaatgg
     6121 gccatttcaa atcgagtcaa ttgtgtaaat gtaaattctt gtgctcctgt ggtgaatcta
     6181 tctgagaaac caggaattgt ttccttaact ctggcacata tggaccagag cttggagaaa
     6241 gaacatggag aacatcttga gtttaacata tcgtcctgga gtgtgaatag cagagaaaac
     6301 cctgaggagg atgttgtaat aaatagcaag gattatatct ttaatgacct ggaatcaggg
     6361 cagatctact gtttccaggt ggagtatttg ctctaccata agccatatgg aaaggctagc
     6421 aaggaaagat gcgttttcat ccctgaaaca cctgaagcaa aaaagaaacg tgttttgatc
     6481 tacggtccac tgatcacatg ttttgttttg atggtgtgcg gtttctgcat ttttctattt
     6541 tgtaaatgca agagtaataa aagagtgcaa agtgtggtta aagagtggtt ccagccattc
     6601 aagctggacc ttcctgacca ctataaagag tttctgtcca gcgagtttcc tgttggattg
     6661 gctccaagtc ccagtattcc aagcttacag tcagatgact ttattatatt aaaggagaac
     6721 gctgacgtgg aggagaatgg gcaagaagcg gaacaacaga agactgaaat attatgattg
     6781 tttttatgtg cctaataatt cctttgcgat ttaacataat ttgctcatca ctaacaagct
     6841 tacgaatcca gatttgactt gtgtaagcat taatagtttt cagtgaagtg acctgtgcca
     6901 ggacctggta ggcaaaaaaa tgggtcaaaa tggctgctac actttgaact ccataaaaaa
     6961 tgcagcacaa actagtaaca tttttatctg tttcaagcta tgagtatttg gatcgcatat
     7021 gatcacaaac tgtaaaatgt gtgcacttgc aatcaatatg ctgcacgtgc agtcatttaa
     7081 aaccctctca gagatccaat tcttacataa tgttatcttt tagatattcc tcaacaattg
     7141 aatcgtttgt gagggcgttg tagcacgttc gtgtctgctt ttatctacct gcgaggtaga
     7201 attgaaactg tataaaaagc atacaaacga tttgaccctt ataggtacca tggtttgtaa
     7261 aaatccttaa atatctcctt tacggttgtt ctgtttctga aggaattcag aatacagtgt
     7321 tgctgttatt gaatgctttt ttttatttgt ccatctcaat gtctagcact taacactaaa
     7381 tctgaaatgc actgtttgta gccagagtcc taggaccgtt tgatgtctga aatttcaatc
     7441 tgtgtatgtg ttgtgggggt aagttgggct tcaccgtact atccttcaac attattattt
     7501 tgtaaattat aaaagaaatc tgcaaatcac tgtaataata aactagtttt tgtttgtcac
     7561 taacagtaac aggctgctta atttaaccgt ctactattct ttccctatta aaatttaatt
     7621 acaagacagt tctcagtatt gcatgcaaca ttgttttttt ttaaattctt tctaaatatc
     7681 agtatgctga tgttctgtgt gaaggagatg aacatttact ttaatgtctg ccataatata
     7741 ttactatact taatgtcaat ttccgataca taattgctgt acatttcatt tggttgccat
     7801 agtggattca tgctgtgaaa aaatatgagt atgaggaact ggagctgttt cagggcttat
     7861 ttaaatgatt aaatgttttg tgtgtgttta tgttctttta aaagttatag tgacagtgcc
     7921 ttcatgctgt ttcagaatta aaaccaacat aaaatgtaaa gatttggaat tgcattgtgc
     7981 tttttttttt attattgtat cgaaatgttc acactgtgta ggtcagagat cagtctttct
     8041 tatttatttt gtgcagtttt gctgtttttg agccacctta ggagggtgta cagtttcaca
     8101 agtggcctct tcaaaaaaag gaactaaata aagtaataat acaaaaaaaa aaaaaaaaaa
     8161 aagcggccgc ttaattaacc tcgagatcca ctagttctag agcggccgcg acttaactag
     8221 ccgccaccat ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca
     8281 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     8341 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     8401 gggacatcct gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg
     8461 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     8521 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     8581 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     8641 agaagaccat gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga
     8701 agggcgagat caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca
     8761 agaccaccta catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca
     8821 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg
     8881 agggccgcca ctccaccggc gcctccggac tcagatctaa gctgaaccct cctgatgaga
     8941 gtggccccgg ctgcatgagc tgcaagtgtg tgctctcctg aggatcgatc caccggatct
     9001 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     9061 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     9241 gttaagatta ccctgttatc cctagagctc cagcttttgt tccctttagt gagggttaat
     9301 tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9361 aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9421 gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9721 gtttttccat aggctcggcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9781 gtggcgaaac ccgacaggac tataaagata ccaggcgttc ccccctggaa gctccctcgt
     9841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9901 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10141 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    10201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10381 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10441 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10501 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactgcccgt
    10561 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10621 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10681 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10741 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10801 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10861 atcaaggcga gttacatgat cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc
    10921 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatgctta tggcagcact
    10981 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11041 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11101 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11161 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11221 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11281 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11341 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11401 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11461 aaaagtgcca cctg
//
