LOCUS       40924_3901       10914 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAct26CRFB1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Levraud JP, Boudinot P, Colin I, Benmansour A, Peyrieras N, Herbomel
            P, Lutfalla G.
  TITLE     Identification of the zebrafish IFN receptor: implications for the 
            origin of the vertebrate IFN system
  JOURNAL   J. Immunol. 178 (7), 4385-4394 (2007)
  PUBMED    17371995
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Lutfalla G.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2006) UMR5124, CNRS/UMII, place Eugene Bataillon, 
            Montpellier cedex 5 34095, France
REFERENCE   3  (bases 1 to 10914)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10914)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Immunol."; date: "2007"; volume: "178"; issue: "7"; pages: 
            "4385-4394"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-SEP-2006) UMR5124, CNRS/UMII, place Eugene Bataillon, 
            Montpellier cedex 5 34095, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5745..7385
                     /codon_start=1
                     /product="zebrafish IFNLR1"
                     /label=zebrafish IFNLR1
                     /note="zCRFB1; helical cytokine receptor; transmembrane"
                     /protein_id="ABJ97324.1"
                     /translation="MKTSSSYTGRLFLIHLYFTVIYAIPAPVNFTIWSHNFRHILHWNY
                     GVNSPPQSVFNLQRFEKGKQTEHLNIRNTTMDVSEACQDIYIPCTFLIWASLDNMNSSV
                     IEKKFIPYEDTIIGPPVIFLSGCGDCLNISISLPNESRKDDKLRRFYNSVSYSISWKKH
                     GNNEVREILRSQEPSKQYVLENLQPGVQYCVKVLPQINSNQNTQPSSWQCEYTSKEEAQ
                     RVLYFMSWLLGATLSGSCVMMLAWILVYTGFLCKPKNPPLKSLSNIVPATYLIPEQTLS
                     ESLSLTEVQLIHLSYDTTENNSQLKNPEKREYEGSVLKSDTEDEEDSNDEDEVKCVYMS
                     CEIDDISCQNNSTKKDIVSALPFNKSSIVCYTKEPAMEKIQYDDLKSEKELLSELFEEG
                     HGHLEVTNTQLKTEGNEKGSTNTPGNINLFSVTVRTFGAEDDLDKEDVYKPLLPKLFVK
                     TDKWTEELLPVSNKIQISIPIETGMKTDDLIHLDSQNDLVHVCSLAHNVETNPDTTMSA
                     YMMNHIGKMHDQNKDLLEETNCDTSYITR"
     primer_bind     complement(7625..7641)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7669..8343
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     polyA_signal    8555..8676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8723..8741)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8762..8778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8786..8802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8810..8840)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8855..8876)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9164..9752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9926..10783)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10784..10888)
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtactaggg
      661 ataacagggt aatctcgagc ggccgccagt gtgatggata tctgcagaat tcgccctttc
      721 gactgggtta gttggcattt ctgtgtggac tttgtatgct ctccctgtgt ttgagtgggt
      781 ttcctccggg tgctccagta tcccccacag tccaaagaca tgcgtataag tgtccgtagt
      841 gaatgagtgt gtgttttcca gtgatgggat gcagctggaa gggaatctgc tgcgtaaaac
      901 atatgctgaa taagttggtg gttcattccg cagtggcgac cccagattaa caaagggact
      961 gagccaaaaa gaaaaggaat aaatgaatga tctctataaa ttaatctatg tatgttttca
     1021 atcaaataac tgaattttca ttaagggaag ctgttaaaat ggagaatctc ctttacataa
     1081 tttaactgtg tgcgaaaaga gaaactgaat ctttaaatgc ctgagtttga gcatgttctg
     1141 acacacttac attcctggaa tgacagccac taaatactgc cttcctctcc tttttagcaa
     1201 agatgcgttt gtaaccctat acattgaatt caattgcagg gtgcaatatt tctttcttga
     1261 cttctccttg gttttattta catttatacc ttcttaaaat acattttggc atcactggtt
     1321 ccatgaagaa cctttaaaag aggctttcca ttgaacagaa agttctttat cagtgtgaaa
     1381 aagctctgta gatgatgtaa aatgctcttg acaaaaaatc ctatgtatat ataactgttt
     1441 gctgaaggat tctttagaga aacaaacacg gttcttctat ggcatttaga acctttcaga
     1501 gtgcaacagc ctaattatac tacataaagt tggcttcttc cacatatctt aactaaatgc
     1561 aaagcactga gctctgacat gccctcaaag taagcaactg acacattgcc agccaataaa
     1621 aatacctgca atgtttgtac ttgtggaaaa catattaaaa taattgcacc aaagtaaact
     1681 aatggtgttt ttttaggcat cgatgattcc atcaagaaca tttgaaaccc acagaacatt
     1741 tccattgcac gagatgtacg ttataggggg aagaaacgct gagaaaaagt ttattttcag
     1801 tagaaaaatg ttatttttct tctataaacc acttgttgaa acattttttc aatgtgtaaa
     1861 tgttgtgtac catattacat cagtcactct tagttcccct ctgtcaatct cattttggtt
     1921 acatttccat attaggaatc tacctgtatc agtgcttaga gcgagcagcc tcaaccagtc
     1981 catcccatca gaactctgag tttgttacct gttggtgttt ccatataggg tgctccaggt
     2041 ctgatgggtg gagacagtca gtaggccact tctctgtgta atattattgt gttctttcat
     2101 tctcctatgc tgtatacagt aaacaacaga gcagatgttc agcaaattaa aaaaacgaca
     2161 acatttaata aaagcaacaa caagagagaa tcgtagactc tcaatcactc tgtctaattt
     2221 ccgctgtaag tcacatgact tcgccttggt tgcaaagaac tacaccatgt gactcatcca
     2281 cagattgcct tataaggaga ttggctgaat atctcttaaa ggggccctga acaaagtgtc
     2341 tcagcaaaca agacacaaca caaaggttat ctcatttcct tcatattttt cattgaccta
     2401 tggctttgga agaaaatcat ttttagttgc tgtgtgtaca tatgcaagct cccatcacaa
     2461 taaaacacac ccatatgtca aaacagctgc tcagactaat aaacgaacaa gcaatcatgc
     2521 tttacaagtt taccacagag tttatatgct agacttattt ccacagggct cctaaaatta
     2581 gctttgctca ctttatattt gttttttgat agaagcctca gcaacttcct ctcagatttg
     2641 tcacattgtg ctgtggctgc tgtaacactt agtcgcctct ctctctcttc ctcttcctct
     2701 ttctgtttga aacaacctta taagggcctc agtaatctgc tgatgacaga aatgatagga
     2761 aatcaaaaag ttatcataaa cacatatgca atactttcta gtaatcataa aagcacagta
     2821 cattcaaaaa tgacgtattt ctcactattt actcatcctc aagtggttaa aaaccttaaa
     2881 gagtttcatt tttctgttca aaacacagga gatattttga ggaaagctaa aaacctgtag
     2941 cctaaccatt gacttgcata gtaggaaaaa aatagaaacc ttttcagatt tcctctacag
     3001 ctaaaaagaa actcccctcc accctaaaca ccaagctgaa actcacacac ctaggaaaga
     3061 atgggcccgg tatcaggaat gtagagcact ttgtgaaata atcatgaggc aataaactgt
     3121 acctatgact cattgacctc tgtgatgaag tttatctttt ctataaggct cagtgagtta
     3181 taggtatgac atttggattc tataggtagt caaaattcaa tatttacatc actgcacgcc
     3241 taggatgtgg gggtgggcag atgtattggg tgtcactgct gttggaagca tcattacaga
     3301 cattttctga tgacaagctg ctttcgtcac tagataaatc acacaacaga ctgtttatca
     3361 agtggtgaca tagccctact gacatttcaa taaccatagc tcctaaagat tttaacatta
     3421 aatgcacaat ttagtcctct ttttggtttc atgctgacaa gataagacaa gaattgcaaa
     3481 gccccctgga taaggcatgc tgccacgcgt ctcagattgc ccatgctaaa ggcaggacat
     3541 tataactggc attaacatct atttatgacc ccatggcctc ctaaaaaacc cgaataggta
     3601 aaggatttac atatttcaat gtaaattcat atcaaatcga tctgggacct tgtttagcca
     3661 aaataaacgc tgtgtagtat tttggtttct ttcatgatag catttgttgc taatagtccc
     3721 tacacattta tcaatgtaaa ctagtctttg cttcaaaaca tcagcattta acatttaaga
     3781 atgggcagat tagggagtgg gcagatgtat taggtgtcat cctggtgacg ttttaacagc
     3841 cattgctcct tacgatttta acattaaatg cacaatttag tccccttttt ggtttcatgc
     3901 tgataagata agaattgcaa agcaccatgg atagggcgtg ctgccacgcg tctcagattg
     3961 cccaagctga aggcaggaca tttaaaactg ggattaacac ctatttatga ccccttggtt
     4021 cctctgaaaa accggaatat gctaaagaat ttacatattt tcaatgaaaa gtcatattaa
     4081 atcaatttgg gatctcgttt agcctaaata ggaacccttt ctagtatttt tttttccttg
     4141 aagccattag cctagcctat cagtgtcaca ttactttatc gaatgtaaac taaacagtct
     4201 gtgcttcaaa acattcacga catatagtgt tattcaatag tttatattat cagtcttttt
     4261 aagtaacagg gtatgtaggc tgattttttt tgtgcactct tcagattcca gtaatgcagc
     4321 agcgtgaagc gtcatcggtt tatgacgtaa ttacgcaaaa gccggcttga caggaagtgt
     4381 tgttttctat ggagcaccgt gcccttgttt tgtatcccct gctttgcaga taaccagata
     4441 aaatgaaata tgcttaatac ggttgcatct aacatgccga gcggcgatgt aacagacgcc
     4501 gatctgagca acaacaaaac aatagtcaat atttcattaa tttccgaatg actcctgatc
     4561 ttctgcagct ttacatccat gatattataa acagcaaagc attatgcaca aaagcaacct
     4621 acgattgaat taaacagtgc aatgttcaca aataaagcct tttttaaata tgcttttaca
     4681 ttatgtttta tagagtcacg agaagtacct gtactttaaa tgggagccac gcccacgttg
     4741 ttcatcaaac taacctaata aaatgacaca cttaaaatgt cttctcaaga attaatcgta
     4801 aatgagtcgt tttcaactag ctgtcaaaca ttaactgaaa tgtaaagcag attagtgtat
     4861 aacgttaatc aaataatgat ttcttctctg ttctaccaat aatggctctt gtataacgtt
     4921 agtttacaca ttgttgtcat ttatagagct gaaacataca gtaaaataca atataagttt
     4981 aaatactatt ggacaatgtc gtactgctat gtttaaacgt cctgtcattc ctgcagccta
     5041 agtacagctt atataaatgc aactttacat catgcattgc tcgtttttca cagaaataat
     5101 aaactattta acgttactcg ctgtatgtga ggttatttaa aagtaacgtt aagccacaga
     5161 catgaagtct agcatccttt actgatttaa ctgtgttcca atccaaatga tatacatatc
     5221 cttaattcaa gtgttacatc tgccaatgct gtgtcaaatc aacttatcgt gtatttcctt
     5281 atcaaattat ttcgcctttt cgccgatccc tgacatacat tatttcaacc cagaacttga
     5341 atttcaagca actagcttga aactcgccaa gtggacgacg gctggccaat cagaccgctc
     5401 gctgccgaaa gtttacctta tatggaagga gccggccgca gtgccggcta taaagctgtg
     5461 acccacctca cgctcagcat tgtgagtttt cagtgcacgc tgagaagatc ttcactcaag
     5521 ggcgaattcc agcacactgg cggccgttac tagtggatcg ggatgtagac tattaggcat
     5581 gactcgtaaa tactttatcg tcgataaaat aaaaggattt acagatctgg acaatccctg
     5641 gtggtgtatt gattaggacc tggacatgct gtgaaatcac cacgacttca tcattgctaa
     5701 agtgtgactc tgcttttgtt tgtgaaatat gattggttat caaaatgaag acatcatcat
     5761 catacactgg ccgtcttttt ctcattcatc tttactttac agtgatctat gcaatccctg
     5821 ctcctgtcaa ctttacaata tggtctcaca acttcagaca cattttacac tggaattatg
     5881 gggtaaattc tcctccacag agcgttttca atttacaaag atttgaaaag ggaaagcaaa
     5941 cagagcattt gaacatcaga aatacaacta tggatgtctc tgaagcatgt caagacatct
     6001 acataccctg cactttcctt atttgggcat cgcttgacaa tatgaattca tcagtaattg
     6061 aaaaaaaatt cataccgtat gaagacacta tcataggtcc accagttata tttttgtctg
     6121 gctgtggaga ctgtttgaat atcagcatct ctctacccaa tgaatcaagg aaagacgata
     6181 aactgcgtcg gttttataat tctgtgtcct acagtataag ttggaagaaa catggaaaca
     6241 atgaggtcag agagatctta agatctcaag aaccaagtaa acagtatgtc ctggagaatc
     6301 ttcagcctgg tgttcagtac tgtgttaaag tacttccaca aatcaattcc aaccagaaca
     6361 ctcaaccttc ttcttggcaa tgtgaataca caagcaaaga ggaggcacaa agagtgttat
     6421 attttatgag ctggttacta ggagcgacgt tgtcaggttc atgtgtgatg atgttggcat
     6481 ggattctggt ttacacagga tttctatgca agcccaagaa ccctcctctc aaatcactga
     6541 gtaacattgt tccagccacc tatctgattc ctgaacagac gttgtctgag agtctgtcat
     6601 taactgaggt tcagttaatt catttgtcct atgatactac cgaaaacaac agtcaactga
     6661 aaaatcctga aaaaagggaa tatgaaggca gtgtcctcaa gtcagacact gaggatgagg
     6721 aagacagtaa tgatgaagac gaagtcaaat gtgtctacat gagttgtgaa attgacgaca
     6781 ttagttgtca aaataatagt acaaagaagg atatagtttc agctttgcca tttaataaaa
     6841 gttcaattgt ctgctacaca aaggaaccag ccatggaaaa gatccaatat gatgatctga
     6901 agagtgaaaa ggaattatta tcagagcttt tcgaagaggg acatggacat cttgaagtga
     6961 caaatactca gttaaaaact gagggaaatg agaagggttc aacaaatacc ccagggaaca
     7021 tcaacctatt ttcagtcact gtgaggactt ttggagcaga agatgacttg gataaggaag
     7081 atgtgtataa acctctgcta cccaaattgt ttgtaaaaac agacaaatgg acagaagagc
     7141 tcttacctgt atctaacaag atacagatat ctatacccat agagacaggc atgaaaacag
     7201 atgatctgat tcatttggac agccaaaatg atcttgttca cgtttgctct ttggctcata
     7261 acgtagaaac caaccctgat acaacaatgt ctgcatatat gatgaaccat ataggaaaaa
     7321 tgcatgatca aaataaggac ttattagagg agacaaattg cgacacaagc tatataacac
     7381 ggtaaataca acaatattta tttaaagact tgaaacaagt ttaaaattta ggccaactag
     7441 aagttgccag tggctttgtt ataattaaga tcataagaac tttgtttttt ttgtaataac
     7501 tttgattatt gttataaagt ttgtttagtg ccacaatcac agaatgatca aaaataaatg
     7561 aagaggcaaa gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagcggccgc ttaattaacc
     7621 tcgagatcca ctagttctag agcggccgcg acttaactag ccgccaccat ggcctcctcc
     7681 gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
     7741 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     7801 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctcag
     7861 ttccagtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     7921 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     7981 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     8041 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     8101 gcctccaccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagatgagg
     8161 ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta catggccaag
     8221 aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat cacctcccac
     8281 aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc
     8341 gcctccggac tcagatctaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc
     8401 tgcaagtgtg tgctctcctg aggatcgatc caccggatct agataactga tcataatcag
     8461 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     8521 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     8581 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8641 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gttaagatta ccctgttatc
     8701 cctagagctc cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat
     8761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag
     8821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg
     8881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctcggcc
     9181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9241 tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     9361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9601 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9961 tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata actacgatac
    10021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10321 cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10381 agttggccgc agtgttatca ctcatgctta tggcagcact gcataattct cttactgtca
    10441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctg
//
