LOCUS       40924_3956        5863 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact baculovirus transfer vector for expressing two proteins 
            simultaneously, for use with the BD BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     178..765
                     /label=baculovirus recombination region (ORF603)
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(769..902)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1231..1340)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1488..1579
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     1589..2946
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(3035..3053)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3067..3083)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3698..3802
                     /label=AmpR promoter
     CDS             3803..4660
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4834..5422
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5710..5731
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5746..5776
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5784..5800
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5808..5824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5839..5857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgacaagct ctgtccgttt gctggcaact gcaagggtct caatcctatt tgtaattatt
       61 gaataataaa acaattataa atgctaaatt tgttttttat taacgataca aaccaaacgc
      121 aacaagaaca tttgtagtat tatctataat tgaaaacgcg tagttataat cgctgaggta
      181 atatttaaaa tcattttcaa atgattcaca gttaatttgc gacaatataa ttttattttc
      241 acataaacta gacgccttgt cgtcttcttc ttcgtattcc ttctcttttt catttttctc
      301 ctcataaaaa ttaacatagt tattatcgta tccatatatg tatctatcgt atagagtaaa
      361 ttttttgttg tcataaatat atatgtcttt tttaatgggg tgtatagtac cgctgcgcat
      421 agtttttctg taatttacaa cagtgctatt ttctggtagt tcttcggagt gtgttgcttt
      481 aattattaaa tttatataat caatgaattt gggatcgtcg gttttgtaca atatgttgcc
      541 ggcatagtac gcagcttctt ctagttcaat tacaccattt tttagcagca ccggattaac
      601 ataactttcc aaaatgttgt acgaaccgtt aaacaaaaac agttcacctc ccttttctat
      661 actattgtct gcgagcagtt gtttgttgtt aaaaataaca gccattgtaa tgagacgcac
      721 aaactaatat cacaaactgg aaatgtctat caatatatag ttgctgatga tccagcatga
      781 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      841 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      901 ttccgagttt gtcagaaagc agaccaaaca gcggttggaa taatagcgag aacagagaaa
      961 tagcggcaaa aataataccc gtatcacttt tgctgatatg gttgatgtca tgtagccaaa
     1021 tcgggaaaaa cgggaagtag gctcccatga taaaaaagta aaagaaaaag aataaaccga
     1081 acatccaaaa gtttgtgttt tttaaatagt acataatgga tttccttacg cgaaatacgg
     1141 gcagacatgg cctgcccggt tattattatt tttgacacca gaccaactgg taatggtagc
     1201 gaccggcgct cagctggaat tcagatctgt gattgtaaat aaaatgtaat ttacagtata
     1261 gtattttaat taatatacaa atgatttgat aataattctt atttaactat aatatattgt
     1321 gttgggttga attaaaggtc ccggcatcct caaatgcata atttcatagt cccccttgtt
     1381 gtaagtgatg cgtatttctg aatctttgta aaatagcaca caagactcca acgcgtttgg
     1441 cgttttattt tcttgctcga ctctagagga tcgatccccc ggatctgatc atggagataa
     1501 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt
     1561 aataaaaaaa cctataaata cggatccggt tattagtaca tttattaagc gctagattct
     1621 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
     1681 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
     1741 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
     1801 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
     1861 gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
     1921 aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
     1981 gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt aacatcgggc
     2041 gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
     2101 tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
     2161 gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg
     2221 ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca
     2281 ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat
     2341 aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt
     2401 ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc
     2461 tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga
     2521 tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt
     2581 tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt
     2641 ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata
     2701 atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc
     2761 tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta
     2821 taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc
     2881 gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag
     2941 ctcggatcga tcccgcacgc cgataacaag ccttttcatt tttactacag cattgtagtg
     3001 gcgagacact tcgctgtcgt cgaggagctt attcccttta gtgagggtta atttatatag
     3061 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3121 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3181 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc ccagtagcgg
     3241 cgcattaagc gcggcgggtg ttggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3301 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3361 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3421 tcgacctcca aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     3481 cggttttacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3541 actcacaact aactcggtct attcttttga tttataagga tttttgtcat tttctgctta
     3601 ctggttaaaa aataagctga tttaacaaat atttaacgcg aaatttaaca aaacattaac
     3661 gtttacaatt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttctttatt
     3721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3781 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3841 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3901 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3961 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4021 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4081 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4141 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4201 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4261 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4321 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4381 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4441 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4501 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4561 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4621 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4681 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4741 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4981 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5221 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5521 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5581 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5641 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     5701 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     5761 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     5821 tgaccatgat tacgaattta atacgactca ctatagggat tcc
//
