LOCUS       V009591                 8532 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009591
VERSION     V009591
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 8532)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8532)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
            Meadow Circle, Palo Alto, CA 94303, USA"
            SGRef: number: 3; type: "Journal Article"
            This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International
            customers, please contact your local distributor. For technical
            information, call (415) 424- 8222 or (800) 662-2566, extension 3.
            This sequence has been compiled from information in the sequence
            databases, published literature and other sources, together with
            partial sequences obtained by CLONTECH. If you suspect there is an
            error in this sequence, please contact CLONTECH's Technical Service
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..8532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(2102..2236)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2565..2674)
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        2822..2913
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     2925..4282
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5426..5442)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5655..6110
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6392..6496
                     /label="AmpR promoter"
     CDS             6497..7354
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7528..8116
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8404..8425
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8440..8470
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8478..8494
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8502..8518
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc
     2101 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2161 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2221 ctgcaataaa caagttccga gtttgtcaga aagcagacca aacagcggtt ggaataatag
     2281 cgagaacaga gaaatagcgg caaaaataat acccgtatca cttttgctga tatggttgat
     2341 gtcatgtagc caaatcggga aaaacgggaa gtaggctccc atgataaaaa agtaaaagaa
     2401 aaagaataaa ccgaacatcc aaaagtttgt gttttttaaa tagtacataa tggatttcct
     2461 tacgcgaaat acgggcagac atggcctgcc cggttattat tatttttgac accagaccaa
     2521 ctggtaatgg tagcgaccgg cgctcagctg gaattcagat ctgtgattgt aaataaaatg
     2581 taatttacag tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa
     2641 ctataatata ttgtgttggg ttgaattaaa ggtcccggca tcctcaaatg cataatttca
     2701 tagtccccct tgttgtaagt gatgcgtatt tctgaatctt tgtaaaatag cacacaagac
     2761 tccaacgcgt ttggcgtttt attttcttgc tcgactctag aggatcgatc ccccggatct
     2821 gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
     2881 cgtaacagtt ttgtaataaa aaaacctata aatatcggat ccgggttatt agtacattta
     2941 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
     3001 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
     3061 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
     3121 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
     3181 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
     3241 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
     3301 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata aagcttggac
     3361 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
     3421 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
     3481 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg
     3541 cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt
     3601 agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg
     3661 tggtggagct gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg
     3721 cggaggcgga ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg
     3781 caacacagtc ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg
     3841 tctgagacga gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc
     3901 taaaggtgca gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat
     3961 cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg
     4021 cggtgccgcc ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg
     4081 cgcaggcgcc gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg
     4141 tggcaattca atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat
     4201 ttcgctatcg tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta
     4261 aagagattgt ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta
     4321 ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt
     4381 caaaaacgtc gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg
     4441 tgttgtcgta aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa
     4501 attgatgcgc atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat
     4561 atctacgatt cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg
     4621 aaaactgcaa aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat
     4681 ctattttatc gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca
     4741 attttaacgc tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa
     4801 aaatctattt taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact
     4861 gtcccgattt atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca
     4921 atattattag acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca
     4981 acgacataaa actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg
     5041 attacggatt gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt
     5101 ttagtccgga aaaaattcga cacacaacta tgcacgtttc gtttgactgg tacgcggcgt
     5161 gttaacatac aagttgctaa ccggcggccg acacccattt gaaaaaagcg aagacgaaat
     5221 gttggacttg aatagcatga agcgtcgtca gcaatacaat gacattggcg ttttaaaaca
     5281 cgttcgtaac gttaacgctc gtgactttgt gtactgccta acaagataca acatagattg
     5341 tagactcaca aattacaaac aaattataaa acatgagttt ttgtcgtaaa aatgccactt
     5401 gttttacgag tagaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5461 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5521 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5581 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     5641 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5701 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5761 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5821 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     5881 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5941 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     6001 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6061 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     6121 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     6181 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6241 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     6301 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     6361 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6421 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6481 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6541 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6601 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6661 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6721 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6781 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6841 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6901 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6961 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7021 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7081 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7141 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7201 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7261 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7321 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7381 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7441 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7501 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7561 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7621 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     7681 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7741 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7801 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7861 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7921 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7981 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8041 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8101 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8161 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8221 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8281 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8341 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8401 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8461 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8521 tgattacgaa tt
//
