LOCUS       V011676                 8813 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011676
VERSION     V011676
KEYWORDS    pAcSecG2T
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     2254..2313
                     /label="GP64 signal sequence"
                     /note="signal sequence from the baculovirus envelope
                     glycoprotein GP64"
     CDS             2320..2973
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2980..2997
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_recomb     3034..4391
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5535..5551)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5764..6219
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6501..6605
                     /label="AmpR promoter"
     CDS             6606..7463
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7637..8225
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8513..8534
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8549..8579
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8587..8603
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8611..8627
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatcta tgctactagt aaatcagtca
     2221 caccaaggct tcaataagga acacacaagc aagatggtaa gcgctattgt tttatatgtg
     2281 cttttggcgg cggcggcgca ttctgccttt gcggatctga tgtcccctat actaggttat
     2341 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa
     2401 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa
     2461 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag
     2521 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa
     2581 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg
     2641 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct
     2701 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat
     2761 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca
     2821 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca
     2881 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa
     2941 gccacgtttg gtggtggcga ccatcctcca aaatcggatc tggttccgcg tggatccccg
     3001 ggaattcatc gtgactgacg atctgagatc cgggttatta gtacatttat taagcgctag
     3061 attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt
     3121 tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc
     3181 tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca
     3241 agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa
     3301 aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt
     3361 gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt
     3421 cgttagtgta caattgactc gacgtaaaca cgttaaataa agcttggaca tatttaacat
     3481 cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag
     3541 ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca
     3601 cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt
     3661 gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg
     3721 gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg
     3781 atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag
     3841 gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg
     3901 gcacctcaac tattgtactg gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag
     3961 tgcgattttt ttcgtttcta atagcttcca acaattgttg tctgtcgtct aaaggtgcag
     4021 cgggttgagg ttccgtcggc attggtggag cgggcggcaa ttcagacatc gatggtggtg
     4081 gtggtggtgg aggcgctgga atgttaggca cgggagaagg tggtggcggc ggtgccgccg
     4141 gtataatttg ttctggttta gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg
     4201 ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa
     4261 tattataatt ggaatacaaa tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt
     4321 ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc
     4381 tcaagctcgg atcgatcccg cacgccgata acaagccttt tcatttttac tacagcattg
     4441 tagtggcgag acacttcgct gtcgtcgacg tacatgtatg ctttgttgtc aaaaacgtcg
     4501 ttggcaagct ttaaaatatt taaaagaaca tctctgttca gcaccactgt gttgtcgtaa
     4561 atgttgtttt tgataatttg cgcttccgca gtatcgacac gttcaaaaaa ttgatgcgca
     4621 tcaattttgt tgttcctatt attgaataaa taagattgta cagattcata tctacgattc
     4681 gtcatggcca ccacaaatgc tacgctgcaa acgctggtac aattttacga aaactgcaaa
     4741 aacgtcaaaa ctcggtataa aataatcaac gggcgctttg gcaaaatatc tattttatcg
     4801 cacaagccca ctagcaaatt gtatttgcag aaaacaattt cggcgcacaa ttttaacgct
     4861 gacgaaataa aagttcacca gttaatgagc gaccacccaa attttataaa aatctatttt
     4921 aatcacggtt ccatcaacaa ccaagtgatc gtgatggact acattgactg tcccgattta
     4981 tttgaaacac tacaaattaa aggcgagctt tcgtaccaac ttgttagcaa tattattaga
     5041 cagctgtgtg aagcgctcaa cgatttgcac aagcacaatt tcatacacaa cgacataaaa
     5101 ctcgaaaatg tcttatattt cgaagcactt gatcgcgtgt atgtttgcga ttacggattg
     5161 tgcaaacacg aaaactcact tagcgtgcac gacggcacgt tggagtattt tagtccggaa
     5221 aaaattcgac acacaactat gcacgtttcg tttgactggt acgcggcgtg ttaacataca
     5281 agttgctaac cggcggccga cacccatttg aaaaaagcga agacgaaatg ttggacttga
     5341 atagcatgaa gcgtcgtcag caatacaatg acattggcgt tttaaaacac gttcgtaacg
     5401 ttaacgctcg tgactttgtg tactgcctaa caagatacaa catagattgt agactcacaa
     5461 attacaaaca aattataaaa catgagtttt tgtcgtaaaa atgccacttg ttttacgagt
     5521 agaattagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5581 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5641 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     5701 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     5761 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5821 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5881 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5941 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     6001 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6061 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     6121 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6181 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     6241 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6301 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6361 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     6421 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6481 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6541 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6601 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6661 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6721 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6781 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6841 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6901 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6961 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7021 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7081 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7141 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7201 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7261 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7321 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7381 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7441 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7501 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7561 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     8041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8281 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8341 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8401 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8461 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8521 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8581 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat
     8641 tkyrsrcstr anngcntact nrmatnntrd ctntwcytmt rysrvcsbrc hrbdcrtcat
     8701 sandrgatry dcmntstsrt nkscntacta sasrrsntat vwbnarsbad tsbdacsdva
     8761 badtsbdacs trsrcbrary mrnrmatnas kbdnwsandv ntssgnrmad ats
//
