LOCUS       pAcSG2.        5544 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact baculovirus transfer vector for expressing proteins using 
            the polyhedrin promoter, for use with the BD BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pAcSG2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This vector accepts inserts as large as 8 kb.
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..588
                     /label=baculovirus recombination region (ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        592..683
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    690..751
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             710..712
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_recomb     771..2226
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(3137..3153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3161..3177)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3185..3215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3230..3251)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3539..4127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4301..5158)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5159..5263)
                     /label=AmpR promoter
ORIGIN
        1 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
       61 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      121 ctcctcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      181 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      241 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      301 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      361 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      421 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      481 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      541 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tatcatggag
      601 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
      661 ttgtaataaa aaaacctata aatacggatc tcgaggaatt caggcctcca tgggagctcg
      721 cggccgcctg cagggtaccc ccgggagatc tgtaccgact ctgctgaaga ggaggaaatt
      781 ctccttgaag tttccctggt gttcaaagta aaggagtttg caccagacgc acctctgttc
      841 actggtccgg cgtattaaaa cacgatacat tgttattagt acatttatta agcgctagat
      901 tctgtgcgtt gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta
      961 taatctttag ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg
     1021 aatttaaata ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag
     1081 ggttgttttt ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa
     1141 cttgccaaat cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt
     1201 aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg
     1261 ttagtgtaca attgactcga cgtaaacacg ttaaataaag cttggacata tttaacatcg
     1321 ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt
     1381 gcttccgaag acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg
     1441 tccgcgatca aatttgtagt tgagcttttt ggaattattt ctgattgcgg gcgtttttgg
     1501 gcgggtttca atctaactgt gcccgatttt aattcagaca acacgttaga aagcgatggt
     1561 gcaggcggtg gtaacatttc agacggcaaa tctactaatg gcggcggtgg tggagctgat
     1621 gataaatcta ccatcggtgg aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt
     1681 ggtggcggtg atgcagacgg cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc
     1741 acctcaacta ttgtactggt ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg
     1801 cgattttttt cgtttctaat agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg
     1861 ggttgaggtt ccgtcggcat tggtggagcg ggcggcaatt cagacatcga tggtggtggt
     1921 ggtggtggag gcgctggaat gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt
     1981 ataatttgtt ctggtttagt ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct
     2041 ggctgcacaa cggaaggtcg tctgcttcga ggcagcgctt ggggtggtgg caattcaata
     2101 ttataattgg aatacaaatc gtaaaaatct gctataagca ttgtaatttc gctatcgttt
     2161 accgtgccga tatttaacaa ccgctcaatg taagcaattg tattgtaaag agattgtctc
     2221 aagctccgca cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac
     2281 acttcgctgt cgtcgacgta catgtatgct ttgttgtcaa aaacgtcgtt ggcaagcttt
     2341 aaaatattta aaagaacatc tctgttcagc accactgtgt tgtcgtaaat gttgtttttg
     2401 ataatttgcg cttccgcagt atcgacacgt tcaaaaaatt gatgcgcatc aattttgttg
     2461 ttcctattat tgaataaata agattgtaca gattcatatc tacgattcgt catggccacc
     2521 acaaatgcta cgctgcaaac gctggtacaa ttttacgaaa actgcaaaaa cgtcaaaact
     2581 cggtataaaa taatcaacgg gcgctttggc aaaatatcta ttttatcgca caagcccact
     2641 agcaaattgt atttgcagaa aacaatttcg gcgcacaatt ttaacgctga cgaaataaaa
     2701 gttcaccagt taatgagcga ccacccaaat tttataaaaa tctattttaa tcacggttcc
     2761 atcaacaacc aagtgatcgt gatggactac attgactgtc ccgatttatt tgaaacacta
     2821 caaattaaag gcgagctttc gtaccaactt gttagcaata ttattagaca gctgtgtgaa
     2881 gcgctcaacg atttgcacaa gcacaatttc atacacaacg acataaaact cgaaaatgtc
     2941 ttatatttcg aagcacttga tcgcgtgtat gtttgcgatt acggattgtg caaacacgaa
     3001 aactcactta gcgtgcacga cggcacgttg gagtatttta gtccggaaaa aattcgacac
     3061 acaactatgc acgtttcgtt tgactggtac gcggcgtgtt aacatacaag ttgctaaccg
     3121 gcggttcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5161 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5221 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5281 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     5341 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     5401 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     5461 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     5521 agattgtact gagagtgcac cata
//
