LOCUS       V011677                 9852 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011677
VERSION     V011677
KEYWORDS    pAcMP3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4017..4341
                     /label="basic protein promoter"
                     /note="baculovirus basic protein promoter, which allows
                     expression during the late instead of very late phase of
                     viral infection"
     misc_feature    4342..4405
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4867..6322
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(7233..7249)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7257..7273)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7281..7311)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7326..7347)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7635..8223)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8397..9254)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9255..9359)
                     /label="AmpR promoter"
     primer_bind     9833..9849
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat cagatccccg ggtaccaaat
     4021 tccgttttgc gacgatgcag agtttttgaa caggctgctc aaacacatag atccgtaccc
     4081 gctcagtcgg atgtattaca atgcagccaa taccatgttt tacacgacta tggaaaacta
     4141 tgccgtgtcc aattgcaagt tcaacattga ggattacaat aacatattta aggtgatgga
     4201 aaatattagg aaacacagca acaaaaattc aaacgaccaa gacgagttaa acatatattt
     4261 gggagttcag tcgtcgaatg caaagcgtaa aaaatattaa taaggtaaaa attacagcta
     4321 cataaattac acaatttaaa cggatcccgg gtaccttcta gaattccgga gcggccgctg
     4381 cagatctgat cctttcctgg gacccggcaa gaaccaaaaa ctcactctct tcaaggaaat
     4441 ccgtaatgtt aaacccgaca cgatgaagct tgtcgttgga tggaaaggaa aagagttcta
     4501 cagggaaact tggacccgct tcatggaaga cagcttcccc attgttaacg accaagaagt
     4561 gatggatgtt ttccttgttg tcaacatgcg tcccactaga cccaaccgtt gttacaaatt
     4621 cctggcccaa cacgctctgc gttgcgaccc cgactatgta cctcatgacg tgattaggat
     4681 cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc atcagcctgg ctaagaaggg
     4741 cggcggctgc ccaataatga accttcactc tgagtacacc aactcgttcg aacagttcat
     4801 cgatcgtgtc atctgggaga acttctacaa gcccatcgtt tacatcggta ccgactctgc
     4861 tgaagaggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg agtttgcacc
     4921 agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt attagtacat
     4981 ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa
     5041 taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc
     5101 agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg
     5161 attagtttca aacaagggtt gtttttccga accgatggct ggactatcta atggattttc
     5221 gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt
     5281 ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt
     5341 tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa ataaagcttg
     5401 gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc
     5461 ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat
     5521 tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttatttctga
     5581 ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt cagacaacac
     5641 gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta ctaatggcgg
     5701 cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg ctggcggcgg
     5761 aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa atgtctcttt
     5821 aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt ttggtttgac
     5881 cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt gttgtctgtc
     5941 gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg gcaattcaga
     6001 catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg
     6061 cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac
     6121 cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg
     6181 tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta taagcattgt
     6241 aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag caattgtatt
     6301 gtaaagagat tgtctcaagc tccgcacgcc gataacaagc cttttcattt ttactacagc
     6361 attgtagtgg cgagacactt cgctgtcgtc gacgtacatg tatgctttgt tgtcaaaaac
     6421 gtcgttggca agctttaaaa tatttaaaag aacatctctg ttcagcacca ctgtgttgtc
     6481 gtaaatgttg tttttgataa tttgcgcttc cgcagtatcg acacgttcaa aaaattgatg
     6541 cgcatcaatt ttgttgttcc tattattgaa taaataagat tgtacagatt catatctacg
     6601 attcgtcatg gccaccacaa atgctacgct gcaaacgctg gtacaatttt acgaaaactg
     6661 caaaaacgtc aaaactcggt ataaaataat caacgggcgc tttggcaaaa tatctatttt
     6721 atcgcacaag cccactagca aattgtattt gcagaaaaca atttcggcgc acaattttaa
     6781 cgctgacgaa ataaaagttc accagttaat gagcgaccac ccaaatttta taaaaatcta
     6841 ttttaatcac ggttccatca acaaccaagt gatcgtgatg gactacattg actgtcccga
     6901 tttatttgaa acactacaaa ttaaaggcga gctttcgtac caacttgtta gcaatattat
     6961 tagacagctg tgtgaagcgc tcaacgattt gcacaagcac aatttcatac acaacgacat
     7021 aaaactcgaa aatgtcttat atttcgaagc acttgatcgc gtgtatgttt gcgattacgg
     7081 attgtgcaaa cacgaaaact cacttagcgt gcacgacggc acgttggagt attttagtcc
     7141 ggaaaaaatt cgacacacaa ctatgcacgt ttcgtttgac tggtacgcgg cgtgttaaca
     7201 tacaagttgc taaccggcgg ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8041 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8401 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8461 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8521 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8581 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8641 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8701 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8761 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8821 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8881 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8941 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9001 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9061 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9241 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9301 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9361 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9421 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     9481 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9541 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     9601 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     9661 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     9721 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     9781 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     9841 acggccagtg cc
//
