LOCUS       V011678                 9847 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011678
VERSION     V011678
KEYWORDS    pAcMP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4017..4341
                     /label="basic protein promoter"
                     /note="baculovirus basic protein promoter, which allows
                     expression during the late instead of very late phase of
                     viral infection"
     misc_feature    4346..4400
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4862..6317
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(7228..7244)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7252..7268)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7276..7306)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7321..7342)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7630..8218)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8392..9249)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9250..9354)
                     /label="AmpR promoter"
     primer_bind     9828..9844
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat cagatccccg ggtaccaaat
     4021 tccgttttgc gacgatgcag agtttttgaa caggctgctc aaacacatag atccgtaccc
     4081 gctcagtcgg atgtattaca atgcagccaa taccatgttt tacacgacta tggaaaacta
     4141 tgccgtgtcc aattgcaagt tcaacattga ggattacaat aacatattta aggtgatgga
     4201 aaatattagg aaacacagca acaaaaattc aaacgaccaa gacgagttaa acatatattt
     4261 gggagttcag tcgtcgaatg caaagcgtaa aaaatattaa taaggtaaaa attacagcta
     4321 cataaattac acaatttaaa cggatctgca gcggccgctc cagaattcta gaaggtaccc
     4381 gggatccttt cctgggaccc ggcaagaacc aaaaactcac tctcttcaag gaaatccgta
     4441 atgttaaacc cgacacgatg aagcttgtcg ttggatggaa aggaaaagag ttctacaggg
     4501 aaacttggac ccgcttcatg gaagacagct tccccattgt taacgaccaa gaagtgatgg
     4561 atgttttcct tgttgtcaac atgcgtccca ctagacccaa ccgttgttac aaattcctgg
     4621 cccaacacgc tctgcgttgc gaccccgact atgtacctca tgacgtgatt aggatcgtcg
     4681 agccttcatg ggtgggcagc aacaacgagt accgcatcag cctggctaag aagggcggcg
     4741 gctgcccaat aatgaacctt cactctgagt acaccaactc gttcgaacag ttcatcgatc
     4801 gtgtcatctg ggagaacttc tacaagccca tcgtttacat cggtaccgac tctgctgaag
     4861 aggaggaaat tctccttgaa gtttccctgg tgttcaaagt aaaggagttt gcaccagacg
     4921 cacctctgtt cactggtccg gcgtattaaa acacgataca ttgttattag tacatttatt
     4981 aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt
     5041 cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt
     5101 ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag
     5161 tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca
     5221 acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg
     5281 ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt
     5341 catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat
     5401 atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt
     5461 cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt
     5521 cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg
     5581 ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag
     5641 aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg
     5701 gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg
     5761 gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca
     5821 acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc
     5881 tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta
     5941 aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg
     6001 atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg
     6061 gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg
     6121 caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg
     6181 gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt
     6241 cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa
     6301 gagattgtct caagctccgc acgccgataa caagcctttt catttttact acagcattgt
     6361 agtggcgaga cacttcgctg tcgtcgacgt acatgtatgc tttgttgtca aaaacgtcgt
     6421 tggcaagctt taaaatattt aaaagaacat ctctgttcag caccactgtg ttgtcgtaaa
     6481 tgttgttttt gataatttgc gcttccgcag tatcgacacg ttcaaaaaat tgatgcgcat
     6541 caattttgtt gttcctatta ttgaataaat aagattgtac agattcatat ctacgattcg
     6601 tcatggccac cacaaatgct acgctgcaaa cgctggtaca attttacgaa aactgcaaaa
     6661 acgtcaaaac tcggtataaa ataatcaacg ggcgctttgg caaaatatct attttatcgc
     6721 acaagcccac tagcaaattg tatttgcaga aaacaatttc ggcgcacaat tttaacgctg
     6781 acgaaataaa agttcaccag ttaatgagcg accacccaaa ttttataaaa atctatttta
     6841 atcacggttc catcaacaac caagtgatcg tgatggacta cattgactgt cccgatttat
     6901 ttgaaacact acaaattaaa ggcgagcttt cgtaccaact tgttagcaat attattagac
     6961 agctgtgtga agcgctcaac gatttgcaca agcacaattt catacacaac gacataaaac
     7021 tcgaaaatgt cttatatttc gaagcacttg atcgcgtgta tgtttgcgat tacggattgt
     7081 gcaaacacga aaactcactt agcgtgcacg acggcacgtt ggagtatttt agtccggaaa
     7141 aaattcgaca cacaactatg cacgtttcgt ttgactggta cgcggcgtgt taacatacaa
     7201 gttgctaacc ggcggttcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7261 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7321 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7381 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7441 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8041 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9361 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9421 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     9481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9541 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     9601 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9661 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     9721 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9781 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     9841 cagtgcc
//
