LOCUS       V011679                 8108 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011679
VERSION     V011679
KEYWORDS    pAcHLT C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2230..2247
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2254..2271
                     /codon_start=1
                     /product="recognition site for phosphorylation by protein
                     kinase A"
                     /label="recognition site for phosphorylation by protein"
                     /note="PKA site"
                     /translation="RRASVA"
     CDS             2281..2298
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    2313..2380
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     2400..3858
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5002..5018)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5231..5686
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5968..6072
                     /label="AmpR promoter"
     CDS             6073..6930
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7104..7692
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7980..8001
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8016..8046
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8054..8070
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8078..8094
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatagat
     2221 ccgatgggac atcatcatca tcatcacgga aggagaaggg ccagtgttgc ggcgggaatt
     2281 ttggtccctc gtggaagccc aggactcgat ggcatatgct cgaggaattc aggcctccat
     2341 gggagctcgc ggccgcctgc agggtacccc cgggagatct gtaccgactc tgctgaagag
     2401 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca
     2461 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa
     2521 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
     2581 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
     2641 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
     2701 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
     2761 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
     2821 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
     2881 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ttggacatat
     2941 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
     3001 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
     3061 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg
     3121 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa
     3181 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt
     3241 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga
     3301 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac
     3361 acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg
     3421 agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa
     3481 ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat
     3541 ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt
     3601 gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca
     3661 ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc
     3721 aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg
     3781 ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga
     3841 gattgtctca agctcggatc gatcccgcac gccgataaca agccttttca tttttactac
     3901 agcattgtag tggcgagaca cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa
     3961 aacgtcgttg gcaagcttta aaatatttaa aagaacatct ctgttcagca ccactgtgtt
     4021 gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta tcgacacgtt caaaaaattg
     4081 atgcgcatca attttgttgt tcctattatt gaataaataa gattgtacag attcatatct
     4141 acgattcgtc atggccacca caaatgctac gctgcaaacg ctggtacaat tttacgaaaa
     4201 ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg cgctttggca aaatatctat
     4261 tttatcgcac aagcccacta gcaaattgta tttgcagaaa acaatttcgg cgcacaattt
     4321 taacgctgac gaaataaaag ttcaccagtt aatgagcgac cacccaaatt ttataaaaat
     4381 ctattttaat cacggttcca tcaacaacca agtgatcgtg atggactaca ttgactgtcc
     4441 cgatttattt gaaacactac aaattaaagg cgagctttcg taccaacttg ttagcaatat
     4501 tattagacag ctgtgtgaag cgctcaacga tttgcacaag cacaatttca tacacaacga
     4561 cataaaactc gaaaatgtct tatatttcga agcacttgat cgcgtgtatg tttgcgatta
     4621 cggattgtgc aaacacgaaa actcacttag cgtgcacgac ggcacgttgg agtattttag
     4681 tccggaaaaa attcgacaca caactatgca cgtttcgttt gactggtacg cggcgtgtta
     4741 acatacaagt tgctaaccgg cggccgacac ccatttgaaa aaagcgaaga cgaaatgttg
     4801 gacttgaata gcatgaagcg tcgtcagcaa tacaatgaca ttggcgtttt aaaacacgtt
     4861 cgtaacgtta acgctcgtga ctttgtgtac tgcctaacaa gatacaacat agattgtaga
     4921 ctcacaaatt acaaacaaat tataaaacat gagtttttgt cgtaaaaatg ccacttgttt
     4981 tacgagtaga attagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5041 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5101 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     5161 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     5221 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5281 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5341 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5401 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     5461 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5521 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     5581 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5641 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     5701 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5761 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5821 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5881 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5941 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6001 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6061 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6121 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6181 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6241 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6301 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6361 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6421 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6481 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6541 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6601 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6661 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6721 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6781 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6841 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6901 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7801 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7861 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7921 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7981 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     8041 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     8101 tacgaatt
//
