LOCUS       V011681                 8112 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011681
VERSION     V011681
KEYWORDS    pAcHLT A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2230..2247
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2254..2271
                     /codon_start=1
                     /product="recognition site for phosphorylation by protein
                     kinase A"
                     /label="recognition site for phosphorylation by protein"
                     /note="PKA site"
                     /translation="RRASVA"
     CDS             2281..2298
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    2313..2384
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     2404..3862
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5006..5022)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5235..5690
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5972..6076
                     /label="AmpR promoter"
     CDS             6077..6934
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7108..7696
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7984..8005
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8020..8050
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8058..8074
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8082..8098
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatagat
     2221 ccgatgggac atcatcatca tcatcacgga aggagaaggg ccagtgttgc ggcgggaatt
     2281 ttggtccctc gtggaagccc aggactcgat ggcatatgct cgatcgagga attcaggcct
     2341 ccatgggagc tcgcggccgc ctgcagggta cccccgggag atctgtaccg actctgctga
     2401 agaggaggaa attctccttg aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga
     2461 cgcacctctg ttcactggtc cggcgtatta aaacacgata cattgttatt agtacattta
     2521 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
     2581 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
     2641 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
     2701 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
     2761 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
     2821 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
     2881 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata aagcttggac
     2941 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
     3001 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
     3061 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg
     3121 cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt
     3181 agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg
     3241 tggtggagct gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg
     3301 cggaggcgga ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg
     3361 caacacagtc ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg
     3421 tctgagacga gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc
     3481 taaaggtgca gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat
     3541 cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg
     3601 cggtgccgcc ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg
     3661 cgcaggcgcc gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg
     3721 tggcaattca atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat
     3781 ttcgctatcg tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta
     3841 aagagattgt ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta
     3901 ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt
     3961 caaaaacgtc gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg
     4021 tgttgtcgta aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa
     4081 attgatgcgc atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat
     4141 atctacgatt cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg
     4201 aaaactgcaa aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat
     4261 ctattttatc gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca
     4321 attttaacgc tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa
     4381 aaatctattt taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact
     4441 gtcccgattt atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca
     4501 atattattag acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca
     4561 acgacataaa actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg
     4621 attacggatt gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt
     4681 ttagtccgga aaaaattcga cacacaacta tgcacgtttc gtttgactgg tacgcggcgt
     4741 gttaacatac aagttgctaa ccggcggccg acacccattt gaaaaaagcg aagacgaaat
     4801 gttggacttg aatagcatga agcgtcgtca gcaatacaat gacattggcg ttttaaaaca
     4861 cgttcgtaac gttaacgctc gtgactttgt gtactgccta acaagataca acatagattg
     4921 tagactcaca aattacaaac aaattataaa acatgagttt ttgtcgtaaa aatgccactt
     4981 gttttacgag tagaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5041 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5101 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5161 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     5221 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5281 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5341 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5401 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     5461 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5521 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     5581 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5641 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     5701 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5761 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5821 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5881 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5941 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6001 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6061 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6121 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6181 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6241 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6301 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6361 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6421 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6481 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6541 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6601 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6661 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6721 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6781 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6841 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6901 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6961 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7021 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     7261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7741 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7801 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7861 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7921 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7981 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8041 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8101 tgattacgaa tt
//
