LOCUS       Exported                9767 bp DNA     circular SYN 24-OCT-2025
DEFINITION  Exported.
ACCESSION   V005496
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9767)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9767)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=Tyrosine-protein phosphatase
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=Primase-associated factor LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4189..4248
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     misc_recomb     4776..6231
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(7148..7164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7172..7188)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7196..7226)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7241..7262)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7550..8138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8312..9169)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9170..9274)
                     /label=AmpR promoter
     primer_bind     9748..9764
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcccggg
     4261 taccttctag aattccggag cggccgctgc agatctgatc ctttcctggg acccggcaag
     4321 aaccaaaaac tcactctctt caaggaaatc cgtaatgtta aacccgacac gatgaagctt
     4381 gtcgttggat ggaaaggaaa agagttctac agggaaactt ggacccgctt catggaagac
     4441 agcttcccca ttgttaacga ccaagaagtg atggatgttt tccttgttgt caacatgcgt
     4501 cccactagac ccaaccgttg ttacaaattc ctggcccaac acgctctgcg ttgcgacccc
     4561 gactatgtac ctcatgacgt gattaggatc gtcgagcctt catgggtggg cagcaacaac
     4621 gagtaccgca tcagcctggc taagaagggc ggcggctgcc caataatgaa ccttcactct
     4681 gagtacacca actcgttcga acagttcatc gatcgtgtca tctgggagaa cttctacaag
     4741 cccatcgttt acatcggtac cgactctgct gaagaggagg aaattctcct tgaagtttcc
     4801 ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg tccggcgtat
     4861 taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
     4921 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
     4981 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
     5041 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
     5101 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
     5161 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
     5221 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
     5281 ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
     5341 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
     5401 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
     5461 gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
     5521 actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
     5581 atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
     5641 ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
     5701 gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
     5761 ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
     5821 ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
     5881 ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
     5941 ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
     6001 ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
     6061 ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
     6121 aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
     6181 aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cgcacgccga
     6241 taacaagcct tttcattttt actacagcat tgtagtggcg agacacttcg ctgtcgtcga
     6301 cgtacatgta tgctttgttg tcaaaaacgt cgttggcaag ctttaaaata tttaaaagaa
     6361 catctctgtt cagcaccact gtgttgtcgt aaatgttgtt tttgataatt tgcgcttccg
     6421 cagtatcgac acgttcaaaa aattgatgcg catcaatttt gttgttccta ttattgaata
     6481 aataagattg tacagattca tatctacgat tcgtcatggc caccacaaat gctacgctgc
     6541 aaacgctggt acaattttac gaaaactgca aaaacgtcaa aactcggtat aaaataatca
     6601 acgggcgctt tggcaaaata tctattttat cgcacaagcc cactagcaaa ttgtatttgc
     6661 agaaaacaat ttcggcgcac aattttaacg ctgacgaaat aaaagttcac cagttaatga
     6721 gcgaccaccc aaattttata aaaatctatt ttaatcacgg ttccatcaac aaccaagtga
     6781 tcgtgatgga ctacattgac tgtcccgatt tatttgaaac actacaaatt aaaggcgagc
     6841 tttcgtacca acttgttagc aatattatta gacagctgtg tgaagcgctc aacgatttgc
     6901 acaagcacaa tttcatacac aacgacataa aactcgaaaa tgtcttatat ttcgaagcac
     6961 ttgatcgcgt gtatgtttgc gattacggat tgtgcaaaca cgaaaactca cttagcgtgc
     7021 acgacggcac gttggagtat tttagtccgg aaaaaattcg acacacaact atgcacgttc
     7081 gtttgactgg tacgccgtcg gcgtgttaac atacaagttg ctaaccggcg gccaattcgt
     7141 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7201 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7261 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7321 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9301 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9361 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     9421 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9481 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     9541 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     9601 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     9661 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     9721 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcc
//
