LOCUS       V011685                 8753 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011685
VERSION     V011685
KEYWORDS    pAcGHLT C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2875..2892
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2899..2916
                     /codon_start=1
                     /product="recognition site for phosphorylation by protein
                     kinase A"
                     /label="recognition site for phosphorylation by protein"
                     /note="PKA site"
                     /translation="RRASVA"
     CDS             2926..2943
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    2958..3025
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     3045..4503
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5647..5663)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5876..6331
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6613..6717
                     /label="AmpR promoter"
     CDS             6718..7575
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7749..8337
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8625..8646
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8661..8691
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8699..8715
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8723..8739
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
     2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
     2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
     2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
     2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
     2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
     2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
     2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
     2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
     2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
     2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
     2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
     2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
     2941 agcccaggac tcgatggcat atgctcgagg aattcaggcc tccatgggag ctcgcggccg
     3001 cctgcagggt acccccggga gatctgtacc gactctgctg aagaggagga aattctcctt
     3061 gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct gttcactggt
     3121 ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct agattctgtg
     3181 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
     3241 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
     3301 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
     3361 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
     3421 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
     3481 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
     3541 tacaattgac tcgacgtaaa cacgttaaat aaagcttgga catatttaac atcgggcgtg
     3601 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
     3661 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
     3721 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
     3781 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
     3841 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
     3901 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
     3961 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
     4021 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
     4081 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
     4141 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
     4201 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
     4261 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
     4321 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
     4381 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
     4441 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
     4501 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
     4561 agacacttcg ctgtcgtcga cgtacatgta tgctttgttg tcaaaaacgt cgttggcaag
     4621 ctttaaaata tttaaaagaa catctctgtt cagcaccact gtgttgtcgt aaatgttgtt
     4681 tttgataatt tgcgcttccg cagtatcgac acgttcaaaa aattgatgcg catcaatttt
     4741 gttgttccta ttattgaata aataagattg tacagattca tatctacgat tcgtcatggc
     4801 caccacaaat gctacgctgc aaacgctggt acaattttac gaaaactgca aaaacgtcaa
     4861 aactcggtat aaaataatca acgggcgctt tggcaaaata tctattttat cgcacaagcc
     4921 cactagcaaa ttgtatttgc agaaaacaat ttcggcgcac aattttaacg ctgacgaaat
     4981 aaaagttcac cagttaatga gcgaccaccc aaattttata aaaatctatt ttaatcacgg
     5041 ttccatcaac aaccaagtga tcgtgatgga ctacattgac tgtcccgatt tatttgaaac
     5101 actacaaatt aaaggcgagc tttcgtacca acttgttagc aatattatta gacagctgtg
     5161 tgaagcgctc aacgatttgc acaagcacaa tttcatacac aacgacataa aactcgaaaa
     5221 tgtcttatat ttcgaagcac ttgatcgcgt gtatgtttgc gattacggat tgtgcaaaca
     5281 cgaaaactca cttagcgtgc acgacggcac gttggagtat tttagtccgg aaaaaattcg
     5341 acacacaact atgcacgttt cgtttgactg gtacgcggcg tgttaacata caagttgcta
     5401 accggcggcc gacacccatt tgaaaaaagc gaagacgaaa tgttggactt gaatagcatg
     5461 aagcgtcgtc agcaatacaa tgacattggc gttttaaaac acgttcgtaa cgttaacgct
     5521 cgtgactttg tgtactgcct aacaagatac aacatagatt gtagactcac aaattacaaa
     5581 caaattataa aacatgagtt tttgtcgtaa aaatgccact tgttttacga gtagaattag
     5641 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     5821 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     5881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6061 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     6121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6181 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     6241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6301 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     6361 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6421 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6481 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     6541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6961 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7561 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7621 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7861 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8521 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8581 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8641 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8701 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga att
//
