LOCUS       V011687                 8757 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011687
VERSION     V011687
KEYWORDS    pAcGHLT A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2875..2892
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2899..2916
                     /codon_start=1
                     /product="recognition site for phosphorylation by protein
                     kinase A"
                     /label="recognition site for phosphorylation by protein"
                     /note="PKA site"
                     /translation="RRASVA"
     CDS             2926..2943
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    2958..3029
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     3049..4507
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5651..5667)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5880..6335
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6617..6721
                     /label="AmpR promoter"
     CDS             6722..7579
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7753..8341
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8629..8650
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8665..8695
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8703..8719
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8727..8743
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
     2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
     2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
     2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
     2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
     2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
     2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
     2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
     2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
     2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
     2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
     2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
     2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
     2941 agcccaggac tcgatggcat atgctcgatc gaggaattca ggcctccatg ggagctcgcg
     3001 gccgcctgca gggtaccccc gggagatctg taccgactct gctgaagagg aggaaattct
     3061 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
     3121 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
     3181 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
     3241 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
     3301 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
     3361 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
     3421 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
     3481 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
     3541 agtgtacaat tgactcgacg taaacacgtt aaataaagct tggacatatt taacatcggg
     3601 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
     3661 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
     3721 cgcgatcaaa tttgtagttg agctttttgg aattatttct gattgcgggc gtttttgggc
     3781 gggtttcaat ctaactgtgc ccgattttaa ttcagacaac acgttagaaa gcgatggtgc
     3841 aggcggtggt aacatttcag acggcaaatc tactaatggc ggcggtggtg gagctgatga
     3901 taaatctacc atcggtggag gcgcaggcgg ggctggcggc ggaggcggag gcggaggtgg
     3961 tggcggtgat gcagacggcg gtttaggctc aaatgtctct ttaggcaaca cagtcggcac
     4021 ctcaactatt gtactggttt cgggcgccgt ttttggtttg accggtctga gacgagtgcg
     4081 atttttttcg tttctaatag cttccaacaa ttgttgtctg tcgtctaaag gtgcagcggg
     4141 ttgaggttcc gtcggcattg gtggagcggg cggcaattca gacatcgatg gtggtggtgg
     4201 tggtggaggc gctggaatgt taggcacggg agaaggtggt ggcggcggtg ccgccggtat
     4261 aatttgttct ggtttagttt gttcgcgcac gattgtgggc accggcgcag gcgccgctgg
     4321 ctgcacaacg gaaggtcgtc tgcttcgagg cagcgcttgg ggtggtggca attcaatatt
     4381 ataattggaa tacaaatcgt aaaaatctgc tataagcatt gtaatttcgc tatcgtttac
     4441 cgtgccgata tttaacaacc gctcaatgta agcaattgta ttgtaaagag attgtctcaa
     4501 gctcggatcg atcccgcacg ccgataacaa gccttttcat ttttactaca gcattgtagt
     4561 ggcgagacac ttcgctgtcg tcgacgtaca tgtatgcttt gttgtcaaaa acgtcgttgg
     4621 caagctttaa aatatttaaa agaacatctc tgttcagcac cactgtgttg tcgtaaatgt
     4681 tgtttttgat aatttgcgct tccgcagtat cgacacgttc aaaaaattga tgcgcatcaa
     4741 ttttgttgtt cctattattg aataaataag attgtacaga ttcatatcta cgattcgtca
     4801 tggccaccac aaatgctacg ctgcaaacgc tggtacaatt ttacgaaaac tgcaaaaacg
     4861 tcaaaactcg gtataaaata atcaacgggc gctttggcaa aatatctatt ttatcgcaca
     4921 agcccactag caaattgtat ttgcagaaaa caatttcggc gcacaatttt aacgctgacg
     4981 aaataaaagt tcaccagtta atgagcgacc acccaaattt tataaaaatc tattttaatc
     5041 acggttccat caacaaccaa gtgatcgtga tggactacat tgactgtccc gatttatttg
     5101 aaacactaca aattaaaggc gagctttcgt accaacttgt tagcaatatt attagacagc
     5161 tgtgtgaagc gctcaacgat ttgcacaagc acaatttcat acacaacgac ataaaactcg
     5221 aaaatgtctt atatttcgaa gcacttgatc gcgtgtatgt ttgcgattac ggattgtgca
     5281 aacacgaaaa ctcacttagc gtgcacgacg gcacgttgga gtattttagt ccggaaaaaa
     5341 ttcgacacac aactatgcac gtttcgtttg actggtacgc ggcgtgttaa catacaagtt
     5401 gctaaccggc ggccgacacc catttgaaaa aagcgaagac gaaatgttgg acttgaatag
     5461 catgaagcgt cgtcagcaat acaatgacat tggcgtttta aaacacgttc gtaacgttaa
     5521 cgctcgtgac tttgtgtact gcctaacaag atacaacata gattgtagac tcacaaatta
     5581 caaacaaatt ataaaacatg agtttttgtc gtaaaaatgc cacttgtttt acgagtagaa
     5641 ttagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     5701 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     5761 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     5821 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     5881 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5941 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6001 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6061 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     6121 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6181 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     6241 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6301 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     6361 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     6421 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     6481 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     6541 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6601 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6661 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6781 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6841 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6901 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6961 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7021 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7081 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7141 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7201 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7261 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7321 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7381 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7441 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7501 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7561 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7621 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7861 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8401 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8461 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8521 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8581 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8641 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8701 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaatt
//
