LOCUS       40924_3761        3354 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing AcGFP1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    234..281
                     /label=5' MCS
                     /note="5' MCS"
                     /note="multiple cloning site"
     CDS             289..1005
                     /codon_start=1
                     /label=AcGFP1
                     /note="Aequorea coerulescens GFP"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    1009..1108
                     /label=3' MCS
                     /note="3' MCS"
                     /note="multiple cloning site"
     promoter        1449..1553
                     /label=AmpR promoter
     CDS             1554..2411
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2585..3173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag
      301 ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg agctgaatgg cgatgtgaat
      361 ggccacaagt tcagcgtgag cggcgagggc gagggcgatg ccacctacgg caagctgacc
      421 ctgaagttca tctgcaccac cggcaagctg cctgtgccct ggcccaccct ggtgaccacc
      481 ctgagctacg gcgtgcagtg cttctcacgc taccccgatc acatgaagca gcacgacttc
      541 ttcaagagcg ccatgcctga gggctacatc caggagcgca ccatcttctt cgaggatgac
      601 ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt gaatcgcatc
      661 gagctgaccg gcaccgattt caaggaggat ggcaacatcc tgggcaataa gatggagtac
      721 aactacaacg cccacaatgt gtacatcatg accgacaagg ccaagaatgg catcaaggtg
      781 aacttcaaga tccgccacaa catcgaggat ggcagcgtgc agctggccga ccactaccag
      841 cagaataccc ccatcggcga tggccctgtg ctgctgcccg ataaccacta cctgtccacc
      901 cagagcgccc tgtccaagga ccccaacgag aagcgcgatc acatgatcta cttcggcttc
      961 gtgaccgccg ccgccatcac ccacggcatg gatgagctgt acaagtgagc ggccgcgact
     1021 ctagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     1081 taggcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     1141 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     1201 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     1261 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     1321 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     1381 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2701 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3241 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3301 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
