LOCUS       40924_3721        4150 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless AcGFP1 reporter vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Can be used to monitor transcription from a promoter inserted into 
            the MCS.
FEATURES             Location/Qualifiers
     source          1..4150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             97..813
                     /codon_start=1
                     /label=AcGFP1
                     /note="Aequorea coerulescens GFP"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    938..1059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1066..1521)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1548..1652
                     /label=AmpR promoter
     promoter        1654..2011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2046..2837
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3072..3119
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3448..4036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgccgagctg
      121 ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg atgtgaatgg ccacaagttc
      181 agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      241 tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg tgaccaccct gagctacggc
      301 gtgcagtgct tctcacgcta ccccgatcac atgaagcagc acgacttctt caagagcgcc
      361 atgcctgagg gctacatcca ggagcgcacc atcttcttcg aggatgacgg caactacaag
      421 tcgcgcgccg aggtgaagtt cgagggcgat accctggtga atcgcatcga gctgaccggc
      481 accgatttca aggaggatgg caacatcctg ggcaataaga tggagtacaa ctacaacgcc
      541 cacaatgtgt acatcatgac cgacaaggcc aagaatggca tcaaggtgaa cttcaagatc
      601 cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc actaccagca gaataccccc
      661 atcggcgatg gccctgtgct gctgcccgat aaccactacc tgtccaccca gagcgccctg
      721 tccaaggacc ccaacgagaa gcgcgatcac atgatctact tcggcttcgt gaccgccgcc
      781 gccatcaccc acggcatgga tgagctgtac aagtgagcgg ccgcgactct agatcataat
      841 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      901 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     1081 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1141 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     1201 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     1261 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1321 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1381 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1441 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1501 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1621 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     1681 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1741 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     1801 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     1861 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1921 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     1981 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     2041 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     2101 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     2161 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     2221 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     2281 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     2341 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     2401 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     2461 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     2521 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     2581 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     2641 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     2701 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     2761 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     2821 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     2881 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     2941 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     3001 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     3061 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     3121 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     3181 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     3241 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     3301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4141 cgccatgcat
//
