LOCUS       V011693                10096 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011693
VERSION     V011693
KEYWORDS    pAcAB3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..578
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2661..3290
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3169..3996
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(4000..4133)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(4462..4571)
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(4722..4813)
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        4839..4948
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_recomb     4967..6324
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        7960..8064
                     /label="AmpR promoter"
     CDS             8065..8922
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9096..9684
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9972..9993
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10008..10038
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10046..10062
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10070..10086
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aattctactc gtaaagcgag ttgaaggatc atatttagtt gcgtttatga gataagattg
       61 aaagcacgtg taaaatgttt cccgcgcgtt ggcacaacta tttacaatgc ggccaagtta
      121 taaaagattc taatctgata tgttttaaaa cacctttgcg gcccgagttg tttgcgtacg
      181 tgactagcga agaagatgtg tggaccgcag aacagatagt aaaacaaaac cctagtattg
      241 gagcaataat cgatttaacc aacacgtcta aatattatga tggtgtgcat tttttgcggg
      301 cgggcctgtt atacaaaaaa attcaagtac ctggccagac tttgccgcct gaaagcatag
      361 ttcaagaatt tattgacacg gtaaaagaat ttacagaaaa gtgtcccggc atgttggtgg
      421 gcgtgcactg cacacacggt attaatcgca ccggttacat ggtgtgcaga tatttaatgc
      481 acaccctggg tattgcgccg caggaagcca tagatagatt cgaaaaagcc agaggtcaca
      541 aaattgaaag acaaaattac gttcaagatt tattaattta attaatatta tttgcattct
      601 ttaacaaata ctttatccta ttttcaaatt gttgcgcttc ttccagcgaa ccaaaactat
      661 gcttcgcttg ctccgtttag cttgtagccg atcagtggcg ttgttccaat cgacggtagg
      721 attaggccgg atattctcca ccacaatgtt ggcaacgttg atgttacgtt tatgcttttg
      781 gttttccacg tacgtctttt ggccggtaat agccgtaaac gtagtgccgt cgcgcgtcac
      841 gcacaacacc ggatgtttgc gcttgtccgc ggggtattga accgcgcgat ccgacaaatc
      901 caccactttg gcaactaaat cggtgacctg cgcgtctttt ttctgcatta tttcgtcttt
      961 cttttgcatg gtttcctgga agccggtgta catgcggttt agatcagtca tgacgcgcgt
     1021 gacctgcaaa tctttggcct cgatctgctt gtccttgatg gcaacgatgc gttcaataaa
     1081 ctcttgtttt ttaacaagtt cctcggtttt ttgcgccacc accgcttgca gcgcgtttgt
     1141 gtgctcggtg aatgtcgcaa tcagcttagt caccaactgt ttgctctcct cctcccgttg
     1201 tttgatcgcg ggatcgtact tgccggtgca gagcacttga ggaattactt cttctaaaag
     1261 ccattcttgt aattctatgg cgtaaggcaa tttggacttc ataatcagct gaatcacgcc
     1321 ggatttagta atgagcactg tatgcggctg caaatacagc gggtcgcccc ttttcacgac
     1381 gctgttagag gtagggcccc cattttggat ggtctgctca aataacgatt tgtatttatt
     1441 gtctacatga acacgtatag ctttatcaca aactgtatat tttaaactgt tagcgacgtc
     1501 cttggccacg aaccggacct gttggtcgcg ctctagcacg taccgcaggt tgaacgtatc
     1561 ttctccaaat ttaaattctc caattttaac gcgagccatt ttgatacacg tgtgtcgatt
     1621 ttgcaacaac tattgttttt taacgcaaac taaacttatt gtggtaagca ataattaaat
     1681 atgggggaac atgcgccgct acaacactcg tcgttatgaa cgcagacggc gccggtctcg
     1741 gcgcaagcgg ctaaaacgtg ttgcgcgttc aacgcggcaa acatcgcaaa agccaatagt
     1801 acagttttga tttgcatatt aacggcgatt ttttaaatta tcttatttaa taaatagtta
     1861 tgacgcctac aactccccgc ccgcgttgac tcgctgcacc tcgagcagtt cgttgacgcc
     1921 ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg accagcggcg tgccgcacgc
     1981 gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa ggcacgtcgg cctccaagtg
     2041 gcaatattgg caaattcgaa aatatataca gttgggttgt ttgcgcatat ctatcgtggc
     2101 gttgggcatg tacgtccgaa cgttgatttg catgcaagcc gaaattaaat cattgcgatt
     2161 agtgcgatta aaacgttgta catcctcgct tttaatcatg ccgtcgatta aatcgcgcaa
     2221 tcgagtcaag tgatcaaagt gtggaataat gttttctttg tattcccgag tcaagcgcag
     2281 cgcgtatttt aacaaactag ccatcttgta agttagtttc atttaatgca actttatcca
     2341 ataatatatt atgtatcgca cgtcaagaat taacaatgcg cccgttgtcg catctcaaca
     2401 cgactatgat agagatcaaa taaagcgcga attaaatagc ttgcgacgca acgtgcacga
     2461 tctgtgcacg cgttccggca cgagctttga ttgtaataag tttttacgaa gcgatgacat
     2521 gacccccgta gtgacaacga tcacgcccaa aagaactgcc gactacaaaa ttaccgagta
     2581 tgtcggtgac gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct
     2641 ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta
     2701 gagcgtcatg tttagacaag aaagctacat atttaattga tcccgatgat tttattgata
     2761 aattgaccct aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg
     2821 gactgcgatt gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca
     2881 attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa
     2941 atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga
     3001 acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa
     3061 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
     3121 ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa
     3181 tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc
     3241 tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa
     3301 aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac
     3361 atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa
     3421 atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact
     3481 agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa
     3541 attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt
     3601 gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct
     3661 gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa
     3721 atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta
     3781 cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc
     3841 caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc
     3901 tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata
     3961 tcacaaactg gaaatgtcta tcaatatata gttgctgatg atccagcatg ataagataca
     4021 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4081 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttccgagtt
     4141 tgtcagaaag cagaccaaac agcggttgga ataatagcga gaacagagaa atagcggcaa
     4201 aaataatacc cgtatcactt ttgctgatat ggttgatgtc atgtagccaa atcgggaaaa
     4261 acgggaagta ggctcccatg ataaaaaagt aaaagaaaaa gaataaaccg aacatccaaa
     4321 agtttgtgtt ttttaaatag tacataatgg atttccttac gcgaaatacg ggcagacatg
     4381 gcctgcccgg ttattattat ttttgacacc agaccaactg gtaatggtag cgaccggcgc
     4441 tcagctggaa ttcagatctg tgattgtaaa taaaatgtaa tttacagtat agtattttaa
     4501 ttaatataca aatgatttga taataattct tatttaacta taatatattg tgttgggttg
     4561 aattaaaggt cccggcatcc tcaaatgcat aatttcatag tcccccttgt tgtaagtgat
     4621 gcgtatttct gaatctttgt aaaatagcac acaagactcc aacgcgtttg gcgttttatt
     4681 ttcttgctcg actctagttt attaggcctc tagagatccg tatttatagg tttttttatt
     4741 acaaaactgt tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa
     4801 ttatctccat gatccaataa acctagaata aagggcccga cctttaattc aacccaacac
     4861 aatatattat agttaaataa gaattattat caaatcattt gtatattaat taaaatacta
     4921 tactgtaaat tacattttat ttacaatcac agatcccggg gatccggtta ttagtacatt
     4981 tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat
     5041 aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca
     5101 gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga
     5161 ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg
     5221 ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt
     5281 tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt
     5341 ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa taaagcttgg
     5401 acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc
     5461 tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt
     5521 gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat
     5581 tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg
     5641 ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc
     5701 ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga
     5761 ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta
     5821 ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt tggtttgacc
     5881 ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg
     5941 tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg caattcagac
     6001 atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga aggtggtggc
     6061 ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc
     6121 ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt
     6181 ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat aagcattgta
     6241 atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc aattgtattg
     6301 taaagagatt gtctcaagct cggatcgatc ccgcacgccg ataacaagcc ttttcatttt
     6361 tactacagca ttgtagtggc gagacacttc gctgtcgtcg acgtacatgt atgctttgtt
     6421 gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga acatctctgt tcagcaccac
     6481 tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc gcagtatcga cacgttcaaa
     6541 aaattgatgc gcatcaattt tgttgttcct attattgaat aaataagatt gtacagattc
     6601 atatctacga ttcgtcatgg ccaccacaaa tgctacgctg caaacgctgg tacaatttta
     6661 cgaaaactgc aaaaacgtca aaactcggta taaaataatc aacgggcgct ttggcaaaat
     6721 atctatttta tcgcacaagc ccactagcaa attgtatttg cagaaaacaa tttcggcgca
     6781 caattttaac gctgacgaaa taaaagttca ccagttaatg agcgaccacc caaattttat
     6841 aaaaatctat tttaatcacg gttccatcaa caaccaagtg atcgtgatgg actacattga
     6901 ctgtcccgat ttatttgaaa cactacaaat taaaggcgag ctttcgtacc aacttgttag
     6961 caatattatt agacagctgt gtgaagcgct caacgatttg cacaagcaca atttcataca
     7021 caacgacata aaactcgaaa atgtcttata tttcgaagca cttgatcgcg tgtatgtttg
     7081 cgattacgga ttgtgcaaac acgaaaactc acttagcgtg cacgacggca cgttggagta
     7141 ttttagtccg gaaaaaattc gacacacaac tatgcacgtt tcgtttgact ggtacgcggc
     7201 gtgttaacat acaagttgct aaccggcggc cgacacccat ttgaaaaaag cgaagacgaa
     7261 atgttggact tgaatagcat gaagcgtcgt cagcaataca atgacattgg cgttttaaaa
     7321 cacgttcgta acgttaacgc tcgtgacttt gtgtactgcc taacaagata caacatagat
     7381 tgtagactca caaattacaa acaaattata aaacatgagt ttttgtcgta aaaatgccac
     7441 ttgttttacg agtagaattc ccagcttggc actggccgtc gttttacaac gtcgtgactg
     7501 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7561 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7621 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7681 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     7741 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     7801 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     7861 cgcggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     7921 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8281 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8581 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9001 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9061 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9241 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9481 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9721 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9781 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9841 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     9901 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     9961 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10021 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10081 tatgaccatg attacg
//
