LOCUS       V009610                 8245 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009610
VERSION     V009610
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive Histone Marks Renewal Impedes Replication Fork
            Progression and Contributes To Late Replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave.
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 8245)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave. 8/2,
            Novosibirsk, NSO 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label="Ac5 promoter"
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2635..5520
                     /gene="SuUR"
                     /label="Protein suppressor of underreplication"
                     /note="Protein suppressor of underreplication from
                     Drosophila melanogaster. Accession#: Q9VTE2"
     regulatory      5528..5537
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5566..5631
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     polyA_signal    complement(5671..5805)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6427..7015)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7189..8046)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8047..8151)
                     /label="AmpR promoter"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccgggccc cccctcgacc tgcaggcggc cgcactagtg atatcccgcg gatcatgtat
     2641 cactttgtat ccgagcaaac gccggaggtg cggctcacag atgaggcact ggtcacgagc
     2701 cacgtgacgc agtacctcaa gagctttcaa ctggatgccg tccgcttcgt ctacgatcgt
     2761 ttggctaagc gggaattctg catcctgaat gatgagagtg gccttggaaa ggtcgctacg
     2821 gtggcggcac ttctaagtgc cctaccaccc gccaaaaaaa cacttgtggt gctccagaac
     2881 gatgagcaac tgctcaccgg ttggcgattc catttggaca ctctcactga cctccaggtg
     2941 tacatcattc agggggtgca agataccacc gactccccgc acagcgttta cctggcgaaa
     3001 tggagccaac tacggagcat tggagatctc agtcgcctca agttcgacta cattatggtg
     3061 gataatcggg ggcattcgct gaacaacagc ttttgcactt ccatgctgct gaagcaattc
     3121 gagggacggg taaacgttct tatctccagt gttgacgtta cgtccgacgt taggttgctc
     3181 tacaatgttc tacggttggg cggccgactg gagcatcagt acaaaagctt tgcgagcttc
     3241 gatcgaaaat tccatctgcc agatccgaag gaggtattca gtaagcgcat agatctcgag
     3301 gaatactaca agcagcgggg attcctcagc gagtacatca aggactttcg tctgcgtcgt
     3361 tttcgtcatc aattcgacaa gtcactgcca cttgtcgcac cggagcaata caaacacaat
     3421 ctgaatctct ggttggcctc aaagaacagc cagagcacaa taagcggctc agatgtgtgc
     3481 tctacaatcg cttccataga caacaatccg gctcagcaaa ataaaactgg gctctttgaa
     3541 gaaacggata ggttatccga gcacagcgtg gatgatgtgg caatgtcacc gctgatattc
     3601 gagtattccg agtctgacga tgaaccccta acagtagaac ctgatgcaga tcaaaacccg
     3661 gtattagttg tatccagcga tgattgtgag attgtaacac ctccaagtac tccgcaaaat
     3721 cgaacgccct cattaaatga atcaccaagg acgaaatcaa agaaaaaatt tagcaaaaaa
     3781 acgtcaccgc gcaaaaaagc tgatctcacc gattccgaag aggatgatga agcgaccgat
     3841 aatatgccgc caaggaagag aactcgtgca gccaccgttc atctgacacc taagacaagg
     3901 agactcaatg ttcgaatttt aagggtttcc ctcgatactc tgagcacacc accaccgtcc
     3961 agaaccacta ctgcgatcgt aacgcccaaa acggagccca cagctagaag aaaaaatctg
     4021 aaaaagcgca cggtgagccc agtggatgtg ggtcgtccag ccacaagggg aatgcagcgc
     4081 ctaacacgat ccgctgaaac aaaaataaac agtaagtact tgaaacatcg ctcattggat
     4141 gatgtcaagc gtagctttcc aaggagagtc aagttggagg gtaaccaaac gcccagaagt
     4201 agcaaacaaa tagtgaagca ggagcctaag tctaaagtcg ggcaggaaaa gaaacagaag
     4261 acggtggatg tacctgctca aggaactgcg aaacgaaagc ctggacgtcc gagaaaatgc
     4321 caaaccaaga ctgaagattt ggggaagaca aagactaagc cgaattcaaa gcacttgcca
     4381 cctactcctc aagttctcag tggtagctcg ttgtcctcgg agtacatgca atgtgctcag
     4441 cgaataccag acaatttgga tgccattgag tcgcccgctt ttcgtgtgcc ctttacacct
     4501 caacaaactc caatgcttct aaccttgccg tctacacaca atttgctcaa cgattctgag
     4561 gtggtgtcta ttccgcttta caaagatcca gttgagacag tggtgatcaa tagctcgcac
     4621 gatgagtgtt ctccgcagga tccatcgcag agtcgtcgca ccaaggcttt gaaaagaaaa
     4681 cgcaaacccg tgacgtccgt caattcaagt ttcggcggcg gattgggtct accaccggcc
     4741 aagaggagtg ccaacaaatc tccagatcta tttagcatat cctcggaaca ctcgcagatt
     4801 ccgctggcgc aaccaagacc ctcatcgccc ttcgaaggct ttaagatctt tggctctgag
     4861 gtgaagcagt ttcagcagca gctagcgaaa gtaaatatat cggtgccaaa aaagaagcga
     4921 gatcgctcgt gtttggatat cctggagcaa atgttcgagc cacgacaaca gcagtcggct
     4981 aaaactagtc ccaaagtctt gccaaccttg ccactgaccc agaaggatga tgcagagtct
     5041 accattacgc agagaagaag aaccctgctg gaagatgact tctttgagat aacgaacaat
     5101 ggccaatttg gcagtcgcat gcgattgaac tcctctggcg aagtatcgcc cgtgcaaccg
     5161 gatcaacagt ccgtcaggcc gtcgcaggcc aataaaatca ccaattattt gataggttct
     5221 ggaattacgc aggagcgaac gcaaccttcg aatggtaatc gcaattccat tcttgcatcg
     5281 ctgcgcaagt cacccaaatc tcccaaacag ggtgctaaat ccactcaggc cacgaaacta
     5341 acacgctggt ttggctccgt cttcggcggt ggagcctcac agaccagctc cgtggaatcg
     5401 gttagtgcac caagtacgcc ggtgaattca tccacaagtg cagcagcttg tcaaacgcga
     5461 tcggcgagaa gtggtggagc atcaggaccg accaaaagaa agcgattgga actgttcaag
     5521 tccgcggcca tggcggccgg gagcgacgtc ggatccacta gcatggacta caaagaccat
     5581 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gctagttcta
     5641 gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag
     5701 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5761 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5821 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     5881 ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa
     5941 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     6001 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     6061 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     6121 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     6181 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     6241 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     6301 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     6361 atcgctcgcg gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6601 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6841 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7321 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7501 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7801 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8161 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8221 taggcgtatc acgaggccct ttcgt
//
