LOCUS       Exported                5351 bp DNA     circular SYN 03-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAc5.1/V5-His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        91..2600
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2692..2733
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2743..2760
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2833..2967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3568..4156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4327..5187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5188..5292)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaatt ctatattcta
       61 aaaacacaaa tgatacttct aaaaaaaaat catgaatggc atcaactctg aatcaaatct
      121 ttgcagatgc acctacttct catttccact gtcacatcat ttttccagat ctcgctgcct
      181 gttatgtggc ccacaaacca agacacgttt tatggccatt aaagctggct gatcgtcgcc
      241 aaacaccaaa tacataatga atatgtacac attcgagaaa gaagcgatca aagaagcgtc
      301 ttcgggcgga gtaggagaat gcggaggaga aggagaacga gctgatctag tatctctcca
      361 caatccaatg ccaactgacc aactggccat attcggagca atttgaagcc aatttccatc
      421 gcctggcgat cgctccattc ttggctatat gtttttcacc gttacccggg gccattttca
      481 aagactcgtc ggcaagataa gattgtgtca ctcgctgtct ctcttcattt gtcgaagaat
      541 gctgaggaat ttcgcgatga cgtcggcgag tattttgaag aatgagaata atttgtattt
      601 atacgaaaat cagttagtgg aattttctac aaaaacatgt tatctataga taattttgtt
      661 gcaaaatatg ttgactatga caaagattgt atgtatatac ctttaatgta ttctcatttt
      721 cttatgtatt tataatggca atgatgatac tgatgatatt ttaagatgat gccagaccaa
      781 aaggcttgaa tttctgcgtc ttttgccgaa cgcagtgcat gtgcaattgt tgttttttgg
      841 aatattcaat tttcggactg tccgctttga tttcagtttc ttggcttatt caaaaagcaa
      901 agtaaagcca aaaaagcgag atggcaatac caaatgcggc aaaacggtag tggaaggaaa
      961 ggggtgcggg gcagcggaag gaagggtggg gcggggcgtg gcggggtctg tggctgggcg
     1021 cgacgtcacc gacgttggag ccactccttt gaccatgtgt gcgtgtgtgt attattcgtg
     1081 tctcgccact cgccggttgt ttttttcttt ttatgctgcg ctctctctag cgccatctcg
     1141 cttacgcatg ctcaacgcac cgcatgttgc cgtttccttt tatgcgtcat tttggctcga
     1201 aataggcaat tatttaaaca aagattagtc aacgaaaacg ctaaaataaa taagtctaca
     1261 atatggttac ttattgccat gtgtgtgcag ccaacgatag caacaaaagc aacaacacag
     1321 gtggctttcc ctctttcact ttttgtttgc aagccgcgtg cgagcaagac ggcacgaccg
     1381 gcaaacgcaa ttacgctgac aaagagcaga cgaagttttg gcgaaaaaca tcaaggcgcc
     1441 tgatacgaat gcatttgcaa taacaattgc gatatttaat attgtttatg aagctgtttg
     1501 acttcaaaac acacaaaaaa aaaaataaaa caaattattt gaaagagaat taggaatcgg
     1561 acgcttatcg ttagggtaac aacaagaaat gcttactgag tcacagcctc tggaaaactg
     1621 ccgcaagcca gagagagaga gaaaaagagg gagagcagct tagaccgcat gtgcttgtgt
     1681 gtgaggcgtc tctctcttcg tctctgttgc gcaaacgcat agactgcact gaaaaaatcg
     1741 attacctatt ttttatgaat gaatatttgc actattacta ttcaaaacta ttaagatagc
     1801 aatcacattc aatagccaaa tactatacca cctgagcgat gcaacgaaat gatcaatttg
     1861 agcaaaaatg ctgcatattt aggacggcat cattatagaa atgcttcttg ctgtgtactt
     1921 ttctctcgtc tggcagctgt ttcgccgtta ttgttaaaac cggcttaagt taggtgtgtt
     1981 ttctacgact agtgaatgcc ctactagaag atgtgtgttg cacaaaatgt ccctggaata
     2041 accaatttga agtgcagata gcagtaaacg taagctaata tgaatattat ttaactgtaa
     2101 tgttttaata tcgctggaca ttactaataa acccactata aacacatgta catatgtatg
     2161 ttttggcata caatgagtag ttggggaaaa aatgtgtaaa agcaccgtga ccatcacagc
     2221 ataaagataa ccagctgaag tatcgaatat gagtaacccc caaattgaat cacatgccgc
     2281 aactgatagg acccatggaa gtacactctt catggcgata tacaagacac acacaagcac
     2341 gaacacccag ttgcggagga aattctccgt aaatgaaaac ccaatcggcg aacaattcat
     2401 acccatatat ggtaaaagtt ttgaacgcga cttgagagcg gagagcattg cggctgataa
     2461 ggttttagcg ctaagcgggc tttataaaac gggctgcggg accagttttc atatcactac
     2521 cgtttgagtt cttgtgctgt gtggatactc ctcccgacac aaagccgctc catcagccag
     2581 cagtcgtcta atccagagac cccggatcgg ggtacctact agtccagtgt ggtggaattc
     2641 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccttcga aggtaagcct
     2701 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
     2761 tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc tcgctgatca
     2821 gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     2881 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2941 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3001 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     3061 gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     3121 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     3181 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     3241 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     3301 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     3361 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     3421 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     3481 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggccagcaaa aggccaggaa
     3541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4321 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4381 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4441 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4501 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4561 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4621 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4681 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4741 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4801 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4861 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4921 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     4981 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5041 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5101 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5161 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5221 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5281 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     5341 catgacatta a
//
