LOCUS       40924_3586        5368 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAc1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Lindner AB, Madden R, Demarez A, Stewart EJ, Taddei F.
  TITLE     Asymmetric segregation of protein aggregates is associated with 
            cellular aging and rejuvenation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 105 (8), 3076-3081 (2008)
  PUBMED    18287048
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Lindner AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2008) U571, INSERM, 156 rue de Vaugirard, Paris 
            75015, France
REFERENCE   3  (bases 1 to 5368)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5368)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2008"; volume: "105"; issue: "8"; pages: 
            "3076-3081"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-2008) U571, INSERM, 156 rue de Vaugirard, Paris 75015, 
            France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2939..3655
                     /codon_start=1
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     protein_bind    complement(3695..3742)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(4417..4519)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4625..4658)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     primer_bind     complement(4974..4990)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattcgag ctcggtaccg cgatcccttg ctgaaaataa catcatcatt
     2281 acgtcgcact gtggcggcta tcgcacttta acgtttcgtg ctgccccctc agtctatgca
     2341 atagaccata aactgcaaaa aaaagtccgc tgataaggct tgaaaagttc atttccagac
     2401 ccatttttac atcgtagccg atgaggacgc gcctgatggg tgttctggct acctgacctg
     2461 tccattgtgg aaggtcttac attctcgctg atttcaggag ctattgatta tgcgtaactt
     2521 tgatttatcc ccgctttacc gttctgctat tggatttgac cgtttgttta accacttaga
     2581 aaacaaccag agccagagta atggcggcta ccctccgtat aacgttgaac tggtagacga
     2641 aaaccattac cgcattgcta tcgctgtggc tggttttgct gagagcgaac tggaaattac
     2701 cgcccaggat aatctgctgg tggtgaaagg tgctcacgcc gacgaacaaa aagagcgcac
     2761 ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc aaattccagt tagctgagaa
     2821 cattcatgtt cgtggtgcta acctggtaaa tggtttgctg tatatcgatc tcgaacgcgt
     2881 gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac gatccgccgg tggcgaccat
     2941 ggctagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     3001 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg ctacatacgg
     3061 aaagcttacc cttaaactta tttgcactac tggaaaacta cctgttccat ggccaacact
     3121 tgtcactact ttgggctatg gtcttcaatg ctttgcccgt tatccggatc atatgaaacg
     3181 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
     3241 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
     3301 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
     3361 actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
     3421 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc aactagcaga
     3481 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     3541 cctgtcgtat caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct
     3601 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaataatg
     3661 aattcgagca tttaaatgga tcccccctag tttcgaagtt cctatacttt ctagagaata
     3721 ggaacttcgg aataggaact tcatttaaat ggcgcgcctt acgccccgcc ctgccactca
     3781 tcgcagtact gttgtattca ttaagcatct gccgacatgg aagccatcac aaacggcatg
     3841 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     3901 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     3961 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     4021 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     4081 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     4141 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgtaa
     4201 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     4261 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     4321 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     4381 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     4441 tctcgacaac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     4501 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     4561 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtaggcg
     4621 cgccgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact aaggaggata
     4681 tcttccctaa ggccgcctgg cgcggcctga catctccatg ctcgccgtca gggagcatat
     4741 gcgaatcttc ggatttgcag gtacttactc gcttcttaga aggagaaatg actatgcgta
     4801 acttcgattt atccccactg atgcgtcaat ggatcggttt tgacaaactg gccaacgcac
     4861 tgcaaaacgc cggtgaaagc cagagcttcc cgccgtacaa cattgagaaa agcgacgata
     4921 accactaccg cattaccctt gcgctggcag gtctgcaggc atgcaagctt ggcactggcc
     4981 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5041 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5101 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     5161 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     5221 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     5281 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     5341 ttttcaccgt catcaccgaa acgcgcga
//
