LOCUS       62056_2660        4828 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             185..1387
                     /codon_start=1
                     /label=AurR
                     /note="aureobasidin A-resistant mutant of S. cerevisiae
                     inositol phosphorylceramide synthase (Aeed et al., 2009)"
                     /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW
                     VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT
                     SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV
                     VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP
                     GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV
                     CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS
                     KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK
                     RA"
     primer_bind     complement(1645..1661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1669..1685)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1693..1723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1738..1759)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2047..2635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2809..3600)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3601..3705)
                     /label=AmpR promoter
     CDS             complement(3812..4612)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4613..4828)
                     /label=URA3 promoter
ORIGIN
        1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat
       61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc
      121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt
      181 aataatggca aacccttttt cgagatggtt tctatcagag agacctccaa actgccatgt
      241 agccgattta gaaacaagtt tagatcccca tcaaacgttg ttgaaggtgc aaaaatacaa
      301 acccgcttta agcgactggg tgcattacat cttcttggga tccatcatgc tgtttgtgtt
      361 cattactaat cccgcacctt ggatcttcaa gatccttttt tattgtttct tgggcacttt
      421 attcatcatt ccagctacgt cacagttttt cttcaatgcc ttgcccatcc taacatgggt
      481 ggcgctgtat ttcacttcat cgtactttcc agatgaccgc aggcctccta ttactgtcaa
      541 agtgttacca gcggtggaaa caattttata cggcgacaat ttaagtgata ttcttgcaac
      601 atcgacgaat tcctttttgg acattttagc atggttaccg tacggactat ttcattatgg
      661 ggccccattt gtcgttgctg ccatcttatt cgtatttggt ccaccaactg ttttgcaagg
      721 ttatgctttt gcatttggtt atatgaacct gtttggtgtt atcatgcaaa atgtctttcc
      781 agccgctccc ccatggtata aaattctcta tggattgcaa tcagccaact atgatatgca
      841 tggctcgcct ggtggattag ctagaattga taagctactc ggtattaata tgtatactac
      901 atgtttttca aattcctccg tcattttcgg tgcttttcct tcactgcatt ccgggtgtgc
      961 tactatggaa gccctgtttt tctgttattg ttttccaaaa ttgaagccct tgtttattgc
     1021 ttatgtttgc tggttatggt ggtcaactat gtatctgaca caccattatt ttgtagacct
     1081 tatggcaggt tctgtgctgt catacgttat tttccagtac acaaagtaca cacatttacc
     1141 aattgtagat acatctcttt tttgcagatg gtcatacact tcaattgaga aatacgatat
     1201 atcaaagagt gatccattgg ctgcagattc aaacgatatc gaaagtgtcc ctttgtccaa
     1261 cttggaactt gactttgatc ttaatatgac tgatgaaccc agtgtaagcc cttcgttatt
     1321 tgatggatct acttctgttt ctcgttcgtc cgccacgtct ataacgtcac taggtgtaaa
     1381 gagggcttaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
     1441 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg
     1501 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
     1561 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cggcctcgac
     1621 tctagcgaat taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     1681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagtcaga agaactcgtc
     2821 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     2881 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     2941 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     3001 attttccacc atgatattcg gcaagcaggc atcgtcatgg gtcacgacga gatcctcgcc
     3061 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     3121 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     3181 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     3241 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     3301 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     3361 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
     3421 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     3481 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     3541 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     3601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3721 agtgccacct gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta
     3781 tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg
     3841 cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa
     3901 atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc
     3961 tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca
     4021 accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa
     4081 aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg
     4141 agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt
     4201 ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa
     4261 tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac
     4321 caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct
     4381 ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta
     4441 gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa
     4501 catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag
     4561 caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc
     4621 ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg caatttcatg
     4681 tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt
     4741 cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa
     4801 aagaataaaa aaaaaatgat gaattgaa
//
