LOCUS       62056_3315       12661 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(310..678)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(711..820)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        1138..1156
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1860..2231
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    2540..3043
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        3044..3230
                     /label=HIS3 promoter
     CDS             3231..3890
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             8393..9106
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        complement(9518..9548)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9563..9584)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9872..10460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             11308..12120
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(12462..12566)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatcgt cttgccttgc tcgtcggtga tgtacttaca gctcgaagtg cctcttcttg
      241 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      301 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      361 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      421 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      481 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      541 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      601 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      661 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      721 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      781 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      841 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      901 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aagatggcca
      961 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1021 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1081 ttcccagtca cgacgttgta aaacgacggc cagtgaccat gattacgcca agctcgaaat
     1141 taaccctcac taaagggaac aaaagctggt acctaacagg attagtgcaa ttcgagttga
     1201 atcactggga aaaacattgt cttctttttt atattatcat ttgcattagt gctgcagtcg
     1261 tagatacttg ttggttgaaa gacatcagct gggagggact ggactagcgt ttggtaagga
     1321 gacatacctg ttaacgttgg ttgcaaaatt ccatttcgcg atttatgtta tctgtaaatc
     1381 ctgatttgtc tggaattctt gatacttccg tttttttaga ggccaatgat tagcatcggc
     1441 gattctcaaa atagcatttt cgacatgcgg tgctgatttc ataaacatag acaacgcttt
     1501 tacatgtaaa agtaacttgc ggacttggaa cagtgctctg tttttggtgt gaacgtaact
     1561 cagcaatatt tctgtgctag caaggttttt tatgatcgac cgaagatctc aaaactccgg
     1621 gtctttcaac tgtctgacta gaccatgttc gtaacgtcgg acagcagctt tcgttgtact
     1681 ggtagaattt ctacgtgcga agcacgtgta ggcaggttga acgacgatcc ctgccgatgg
     1741 atggattggc acgcggcgga acgctttcgt gatctacacc acctggatct tcacatatct
     1801 tcgaaatcga aaaattaacc aagtcgacgg tatcgataat attctagctg agggtaccca
     1861 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     1921 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     1981 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     2041 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     2101 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     2161 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     2221 aggagcagga ctgaccgacg ccgaccaaca ccgccggtcc gacgcggccc gacgggtccg
     2281 aggcctcgga gatctgggcc catgcggccg caacaactac ctcgactttg gctgggacac
     2341 tttcagtgag gacaagaagc ttcagaagcg tgctatcgaa ctcaaccagg gacgtgcggc
     2401 acaaatgggc atccttgctc tcatggtgca cgaacagttg ggagtctcta tccttcctta
     2461 aaaatttaat tttcattagt tgcagtcact ccgctttggt ttcacagtca ggaataacac
     2521 tagctcgtct tcagcgagca tcacgtgcta taaaaataat tataatttaa attttttaat
     2581 ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg
     2641 ttttccgaag atgtaaaaga ctctaggggg atcgccaaca aatactacct tttaccttgc
     2701 tcttcctgct ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca
     2761 cacgaaaatc ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat
     2821 ctgcttttct tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa
     2881 cctttgttta tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta
     2941 aaatccaaat acaaaacata aaaataaata aacacagagt aaattcccaa attattccat
     3001 cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg atcctagtac actctatatt
     3061 tttttatgcc tcggtaatga ttttcatttt ttttttccac ctagcggatg actctttttt
     3121 tttcttagcg attggcatta tcacataatg aattatacat tatataaagt aatgtgattt
     3181 cttcgaagaa tatactaaaa aatgagcagg caagataaac gaaggcaaag atgacagagc
     3241 agaaagccct agtaaagcgt attacaaatg aaaccaagat tcagattgcg atctctttaa
     3301 agggtggtcc cctagcgata gagcactcga tcttcccaga aaaagaggca gaagcagtag
     3361 cagaacaggc cacacaatcg caagtgatta acgtccacac aggtataggg tttctggacc
     3421 atatgataca tgctctggcc aagcattccg gctggtcgct aatcgttgag tgcattggtg
     3481 acttacacat agacgaccat cacaccactg aagactgcgg gattgctctc ggtcaagctt
     3541 ttaaagaggc cctaggggcc gtgcgtggag taaaaaggtt tggatcagga tttgcgcctt
     3601 tggatgaggc actttccaga gcggtggtag atctttcgaa caggccgtac gcagttgtcg
     3661 aacttggttt gcaaagggag aaagtaggag atctctcttg cgagatgatc ccgcattttc
     3721 ttgaaagctt tgcagaggct agcagaatta ccctccacgt tgattgtctg cgaggcaaga
     3781 atgatcatca ccgtagtgag agtgcgttca aggctcttgc ggttgccata agagaagcca
     3841 cctcgcccaa tggtaccaac gatgttccct ccaccaaagg tgttcttatg tagttttaca
     3901 caggagtctg gacttgacct ctagaacata ccttcagcgt cgtcttcact gtcacagtca
     3961 actgacagta atcgttgatc cggagagatt caaaattcaa tctgtttgga cctggataag
     4021 acacaagagc gacatcctga catgaacgcc gtaaacagca aatcctggtt gaacacgtat
     4081 ccttttgggg gcctccgcta cgacgctcgc tccagctggg gcttccttac tatacacagc
     4141 gcgcatattt cacggttgcc agaagtcaag gaattcgcat gacttaagga gctcggtacc
     4201 cgggatgact caggacgagc cgaagtgcat tcatgtttcg tttcacgatc aagcggctca
     4261 gactcctgac gccgtctgtc tcatcgaaga agatctgaca ttcacttacg ccgaggtcca
     4321 acgtcgcgtg attctactgg caaaggaact ccgcgacaat ggttcctgta cgaacgctgt
     4381 tgttgccatt ttcatggaac cttgtgcgga ctatatcatc tccatgttgg ctgtgctgac
     4441 ggcgggggcg gcgtacgtgc cgttggaact cgcttatcca atcaccatgt tgcagcgtgt
     4501 gctgcacgat gctacacctg tggtggtggt gactaaacag gaacaacgag cactgctgcc
     4561 cgtgacaaac acggccttgg cggttctttg tctcgacgat aacgaacatc acgagctgca
     4621 ggaaactgcc ggacagccag aatcacaggc agaactattg cagacgtatc agtcctttcc
     4681 tccagtttcg ctggacgatc tcgccttcat tgtgtactcc agtggtacta cgggtcaacc
     4741 caaaggtatt gcaaatccgc accgagctcc ggccctttcg taccgttggc ggtttgacga
     4801 attcgtcgac cctggtccag gcagtattgt agcgtgcaac gttttctttg tttgggaagc
     4861 cctgcgagcc gtcatgcgag gcggagctgt ggtccccgtt cccgcttcaa tcgtctttga
     4921 tggcgaagcc ttatctgtct ttctgcatca acacagcgtt acggaaatgc tatttacacc
     4981 ttcccttttg gaaaactttt tcaataccat gtcggaagcc gatttgcgag cgcgattggt
     5041 tgccttgaag acaattttcc ttaatgggga agtcgtcacc ctgaatttgc gcgaacgttg
     5101 tttccgttta ctaccctccg ttcgcttcat caatctgtat tcgattagtg agtgtcacga
     5161 agtgggtgct gtcgatttgc gcgaaataga tctgaatctt tccaccaagt attgtccgat
     5221 tggtgcccca tgtacctatt cacctgcata cattctagac gatgaaggaa gacacgctgt
     5281 tgcacctggt gatgcgggcg aactctacat tggcggagac atgttggcag tgggttattt
     5341 gaatctacct gaactaacgg ccacccgatt cgtgccggat ccttttcgac ctgatgaagg
     5401 gtgcatgtac cggacaggag atcgtgcgcg aatgttggaa aacggacagc tagaaattct
     5461 tggtcgctgt gattttatgg tgaaaattcg tggatattct atcgtgctag gcgccgtgga
     5521 agcggcactg gtcgaaaccg tctctttgtc gtcgtgtgtg gttgtcgccg acggagaaga
     5581 gggcgaagat aaacacctgg tggcctatct ggtgcgcgca ccccatgagg atgttgaaac
     5641 acgcctcagc cactggtcca ttgatactcg taccggtgct tgcccagaaa ttcgccgcgc
     5701 agtcgacggc gccttgccac attacatggt tcctagtgtt tttgtagaag ttgaaacatt
     5761 gccagtcagt gcggtcggag caaaacttga tcgcaaggca ttacaggcac aatcggccga
     5821 tcgcagggcc atgctccggt ccttgcaatt gtcagccgaa acccacacaa ccccgttaca
     5881 tacggctact agtcatcagc cagcacgctg gaagcgcgtg gcgaaacatt tacgggtacc
     5941 gcatgggtcg agtcgagaag atgtggaaga tgtcatgctc attttatggg aagttgttct
     6001 tgatcgcgag ccaggcatgt tggacagtaa ttccgacttt cacgagcacg gaggccattc
     6061 gcttagtgct gcacgactcg tctctttgat gaataaaacc ttctcttgcc gactactcgc
     6121 agtacagctg atgcaaggaa tgtccatagg cacagcaaca gatgctgtag tggcatcttg
     6181 gttggaagac ccgatctcca atggtgggga atccggaagc aatcgtgtac atcaaatgaa
     6241 tggaagcggc gggacgattc cgaacggcgc gttgaggaca gcagatgaag atcagattat
     6301 ccaacaagta cgtggagctg cggtcttgcc ggaagatatt ataccaaagt ctcagggatt
     6361 tccgactcgt ggtctcggcg agagcaaaga agtatttttg actggatcca caggctttct
     6421 cggagctcac gtgctggctg agctcctact caaatatccg tccgcgacag tggtatgtct
     6481 ggctcgctcc aaagatccta aagttgttca gattaatctg gaacgctaca agctgtggca
     6541 accagaattt tctactcgaa ttaaagccgt cagcggagat ttgtcgcttg cgaagcttgg
     6601 cttggatcta agcagctgga agcaaataac acaggctgct gatgctgtcg tccattgcgg
     6661 agctgctgtg tcactaacat ctccgtatgc aatgcttgaa gctgtgaatg tgtacggcac
     6721 actgaatatt attcgtcttg cttgtgaatg caaagccggc acacctctta tctatgtctc
     6781 gtccaacgga attttcccgt gtgacaaggg caaagatgaa atttttcttg aaaatgatga
     6841 tgttgggtgc ctgccggatc gacttggagc catgaacggt tatgggctta gcaaatgggt
     6901 tgcagagcag cttgttgtcg ctgcgcacaa gcgagggctc cccacaatga caattcgttt
     6961 tggcaatcta ggatggcaat caacttctgg gattggtaac tctttggatt ttcagagtat
     7021 aattctaaat ggcgctcggc gaatggtggt ccggcctcgt gtaaaagggt ggaaattcga
     7081 aatcacgcca atcgattttg ccgcagcagc gctcgtcggt cttgcagaca ctgctataca
     7141 cctaaaagcc gggtctatct ttaattgtgt ccagtcagaa cttgtcgatg cagaccgtgt
     7201 ctttggttgg gtgtccgaga gcgataccct ttctctcttg gcgcttgact tcgaagactg
     7261 gcaacagcgg gtagacgagg cgagcaacga cgacctgtcg ctatccacat tgcaggcctt
     7321 tgccatgggg ctcccaggtg gagcctcgta cttatccgaa tgtgcacatc tagattgcag
     7381 caagttcgat gcagccgtag cctcgcttca tcccccgtta cggcgtcttg gtccttcgga
     7441 actttcggag tatttcaaaa tcttccttag cgccaacccg attatatcgt ctgtggcggc
     7501 cgacagcgtc ataaagccgt ctgcggtcga tccctctgtt tcaactgaac atcaaggtcc
     7561 tctggctggt caagttgccg tcgttacagg cgcctcgtcc ggaattggtc gagcaatcgt
     7621 cctgtcactg gtccaagccg gatgtaatgt tgctatggct gctcgtagat tatctgagct
     7681 cgaaaagact caaaaggaag tagctgaagc gtgcagcggc tctccggtta agatgatgtg
     7741 cgtacgtacg gacgttacga agcgcgacga agtggctcat ttagtacagg ttgtagaagt
     7801 ttctctgggg ccaattgata tcatggtaaa ctgcgccggg gtcatgtact tcactttgat
     7861 gaaaaatgta gtctgggatc agtgggaagc gcaagtggat gtcaactgta agggaacgat
     7921 gtacggaatc ggatctgtac ttcccagaat gctcgatcga ggaaaagggc acatcgtgaa
     7981 cattacaagt gatgccggcc gcaaggcgtt tcctgggttg gcggtgtact ctggttcaaa
     8041 gttttttgtc gaaggggtga gccaggcact tcgcgcggag actgcctcta cagggctccg
     8101 agtgacctgt attcagcctg gtaacgtgga gactcctttg ctctcgaaat caaccgatcc
     8161 cgatgggctc gcagaatatg ggacaccaac tggcgcgaag gttctcgagc cggcagatat
     8221 aggcagggct gtcgtatacg ccgtgtccca gcctgagtgg tgtgcagtaa acgagattct
     8281 cgtcgaacct cgagacgagc ccgccatccg tcgacctgca ggaagcttac tagtagcgga
     8341 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagtgagcaa
     8401 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     8461 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     8521 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     8581 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     8641 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     8701 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     8761 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     8821 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     8881 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     8941 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     9001 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     9061 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggaat tctaacagaa
     9121 gcgtgctatc gaactcaacc agggacgtgc ggcacaaatg ggcatccttg ctctcatggt
     9181 gcacgaacag ttgggagtct ctatccttcc ttaaaaattt aattttcatt agttgcagtc
     9241 actccgcttt ggtttcacag tcaggaataa cactagctcg tcttcaccat ggatgccaat
     9301 ctcgcctatt catggtgtat aaaagttcaa catccaaagc tagaactttt ggaaagagaa
     9361 agaatatccg aatagggcac ggcgtgccgt attgttggag tggactagca gaaagtgagg
     9421 aaggcacagg atgagttttc tcgaggcatg caagcttggc gtaatcatgg tcatagctgt
     9481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11161 gatgcttttc tgtgactggt gagtcgattt attcaacaaa gccacgttgt gtctcaaaat
    11221 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
    11281 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg
    11341 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
    11401 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
    11461 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
    11521 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
    11581 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa
    11641 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
    11701 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
    11761 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
    11821 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
    11881 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
    11941 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
    12001 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
    12061 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
    12121 taatcagaat tggttaattg gttgtaacac tggcactcaa ccaagtcatt ctgagaatag
    12181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    12241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    12301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    12421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    12481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    12541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    12601 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    12661 c
//
