LOCUS       40924_5159        4603 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid vector pAYE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Yee A, Tan F-L., Ginsburg D.
  TITLE     Functional display of platelet-binding VWF fragments on filamentous 
            bacteriophage
  JOURNAL   PLoS ONE 8 (9), E73518 (2013)
  PUBMED    24019925
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Yee A, Tan F-L., Ginsburg D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2013) Life Scieneces Institute, University of 
            Michigan, 210 Washtenaw Ave, Ann Arbor, MI 48109, USA
REFERENCE   3  (bases 1 to 4603)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4603)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "9"; pages: "E73518"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUL-2013) Life Scieneces Institute, University of Michigan, 210 
            Washtenaw Ave, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2269..3723
                     /codon_start=1
                     /transl_except="(pos: 2326 .. 2328,  aa: OTHER)"
                     /product="VWF A1-pIII fusion protein"
                     /label=VWF A1-pIII fusion protein
                     /note="stop codon is suppressed in Eshcerichia coli
                     containing the glnV mutation (supE)"
                     /protein_id="AHB62418.1"
                     /translation="MRVLLFLLLSLFMLPAFSA*PAGAPGGQEPGGLVVPPTDAPVSPT
                     TLYVEDISEPPLHDFYCSRLLDLVFLLDGSSRLSEAEFEVLKAFVVDMMERLRISQKWV
                     RVAVVEYHDGSHAYIGLKDRKRPSELRRIASQVKYAGSQVASTSEVLKYTLFQIFSKID
                     RPEASRIALLLMASQEPQRMSRNFVRYVQGLKKKKVIVIPVGIGPHANLKQIRLIEKQA
                     PENKAFVLSSVDELEQQRDEIVSYLCDLAPEAPPPTLPPDMAQVGGGRAAAAGAPVPYP
                     DPLEPRAAQGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEK
                     MANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAG
                     SNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGV
                     FAFLLYVATFMYVFSTFANILRNKES"
     sig_peptide     2269..3720
     misc_feature    2326..2328
                     /label=amber stop
                     /note="amber stop"
     primer_bind     complement(3733..3749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3962..4417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgct cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcataca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt tggagccttt tttttggaga ttttcaacat gcgtgttctg
     2281 ctgttcctgc tgctgtctct gttcatgctg ccggctttct cggcctagcc ggccggcgcg
     2341 ccaggaggac aggagccggg aggcctggtg gtgcctccca cagatgcccc ggtgagcccc
     2401 accactctgt atgtggagga catctcggaa ccgccgttgc acgatttcta ctgcagcagg
     2461 ctactggacc tggtcttcct gctggatggc tcctccaggc tgtccgaggc tgagtttgaa
     2521 gtgctgaagg cctttgtggt ggacatgatg gagcggctgc gcatctccca gaagtgggtc
     2581 cgcgtggccg tggtggagta ccacgacggc tcccacgcct acatcgggct caaggaccgg
     2641 aagcgaccgt cagagctgcg gcgcattgcc agccaggtga agtatgcggg cagccaggtg
     2701 gcctccacca gcgaggtctt gaaatacaca ctgttccaaa tcttcagcaa gatcgaccgc
     2761 cctgaagcct cccgcatcgc cctgctcctg atggccagcc aggagcccca acggatgtcc
     2821 cggaactttg tccgctacgt ccagggcctg aagaagaaga aggtcattgt gatcccggtg
     2881 ggcattgggc cccatgccaa cctcaagcag atccgcctca tcgagaagca ggcccctgag
     2941 aacaaggcct tcgtgctgag cagtgtggat gagctggagc agcaaaggga cgagatcgtt
     3001 agctacctct gtgaccttgc ccctgaagcc cctcctccta ctctgccccc cgacatggca
     3061 caagtcggag gagggcgcgc cgcggccgca ggtgcgccgg tgccgtatcc ggatccgctg
     3121 gaaccgcgtg ccgcacaggg cggcggctct ggtggtggtt ctggtggcgg ctctgagggt
     3181 ggcggctctg agggtggcgg ctctgagggt ggcggttctg agggtggcgg ctctgagggt
     3241 ggcggttccg gtggcggctc cggttccggt gattttgatt atgaaaaaat ggcaaacgct
     3301 aataaggggg ctatgaccga aaatgccgat gaaaacgcgc tgcagtctga cgctaaaggc
     3361 aaacttgatt ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt
     3421 tccggccttg ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg
     3481 gctcaagtcg gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct
     3541 tctttgcctc agtcggttga atgtcgccct tatgtctttg gcgctggtaa accgtatgaa
     3601 ttttctattg attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat
     3661 gttgccacct ttatgtatgt attttcgacg tttgctaaca tactgcgtaa taaggagtct
     3721 taataagaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3781 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3841 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     3901 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     3961 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4021 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4081 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4141 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     4201 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4261 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     4321 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4381 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcagtct cagtacaatc
     4441 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4501 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4561 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cga
//
