LOCUS       V009386                10639 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009386
VERSION     V009386
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Lu Y, Stegemann S, Agrawal S, Karcher D, Ruf S, Bock R.
  TITLE     Horizontal Transfer of a Synthetic Metabolic Pathway between Plant
            Species
  JOURNAL   Curr. Biol. (2017) In press
   PUBMED   28943084
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   Karcher D, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2017) Organelle Biology, Biotechnology and
            Molecular Ecophysiology, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg 1, Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 10639)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10639)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. Biol.
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-AUG-2017) Organelle Biology, Biotechnology and Molecular
            Ecophysiology, Max Planck Institute of Molecular Plant Physiology,
            Am Muehlenberg 1, Potsdam, Brandenburg 14476, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..54
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      73..201
                     /label="Prrn"
                     /note="Prrn"
                     /regulatory_class="promoter"
     5'UTR           202..243
     CDS             244..1758
                     /codon_start=1
                     /gene="Lyc"
                     /product="lycopene beta-cyclase"
                     /label="Lyc"
                     /note="derived from daffodil"
                     /protein_id="ATG32096.1"
                     /translation="MDTLLRTHNRLELLYPLHELAKRHFLSPSPNPQNPNFKFFSRKPY
                     QKKCRNGYIGVSSNQLLDLVPEIKKEHLEFDLPLYDPSKALTLDLAVVGGGPAGLAVAQ
                     QVSEAGLSVVSIDPNPKLIWPNNYGVWVDEFEDMDLLDCLDATWSGAIVYVDDRSTKNL
                     SRPYARVNRKNLKSKMMKKCVSNGVRFHQATVVKAMHEEEKSYLICSDGVTIDARVVLD
                     ATGFSRCLVQYDKPYNPGYQVAYGILAEVEEHPFDVDKMVFMDWRDSHLNGKAELNERN
                     AKIPTFLYAMPFSSNRIFLEETSLVARPGLKMEDIQERMVARLNHLGIRIKSIEEDERC
                     VIPMGGPLPVIPQRVVGIGGTAGMVHPSTGYMVARTLAAAPIVANSIVQYLVSDSGLSG
                     NDLSADVWKDLWPIERRRQREFFCFGMDILLKLDLEGTRRFFDAFFDLEPRYWHGFLSS
                     RLFLPELVPFGLSLFSHASNTCKLEIMAKGTLPLVNMINNLVQDRD"
     gene            244..1758
                     /gene="Lyc"
                     /label="Lyc"
                     /note="NpLyc"
     regulatory      1769..1918
                     /label="derived from Nicotiana tabacum rps16"
                     /note="derived from Nicotiana tabacum rps16"
                     /regulatory_class="terminator"
     misc_feature    1931..1980
                     /note="IEE; intercistronic expression element"
     CDS             2005..2739
                     /codon_start=1
                     /gene="crtW"
                     /product="beta-carotene ketolase"
                     /label="crtW"
                     /note="derived from marine bacterium Brevundimonas"
                     /protein_id="ATG32097.1"
                     /translation="MTAAVAEPRIVPRQTWIGLTLAGMIVAGWGSLHVYGVYFHRWGTS
                     SLVIVPAIVAVQTWLSVGLFIVAHDAMHGSLAPGRPRLNAAVGRLTLGLYAGFRFDRLK
                     TAHHAHHAAPGTADDPDFYAPAPRAFLPWFLNFFRTYFGWREMAVLTALVLIALFGLGA
                     RPANLLTFWAAPALLSALQLFTFGTWLPHRHTDQPFADAHHARSSGYGPVLSLLTCFHF
                     GRHHEHHLTPWRPWWRLWRGES"
     gene            2005..2739
                     /gene="crtW"
                     /label="crtW"
                     /note="BsCrtW"
     regulatory      2746..2970
                     /label="derived from Nicotiana tabacum rbcL"
                     /note="derived from Nicotiana tabacum rbcL"
                     /regulatory_class="terminator"
     misc_feature    2976..3025
                     /note="IEE; intercistronic expression element"
     CDS             3045..3530
                     /codon_start=1
                     /gene="crtZ"
                     /product="beta-carotene hydroxylase"
                     /label="crtZ"
                     /note="derived from marine bacterium Brevundimonas"
                     /protein_id="ATG32098.1"
                     /translation="MAWLTWIALFLTAFLGMEAFAWIMHRYVMHGFLWSWHRSHHEPHD
                     HPLEKNDLFAVVFAAPAIVMVAVGLHLWPWALPVGLGITAYGMVYFFFHDGLVHRRFPT
                     GFSGRSGFWTRRIQAHRLHHAVRTREGCVSFGFLWVRSARALKAELAQKRGSSSSGA"
     gene            3045..3530
                     /gene="crtZ"
                     /label="crtZ"
                     /note="BsCrtZ"
     regulatory      3537..3758
                     /label="derived from Chlamydomonas rbcL"
                     /note="derived from Chlamydomonas rbcL"
                     /regulatory_class="terminator"
     misc_feature    complement(3792..3825)
                     /label="loxP"
                     /note="loxP"
     protein_bind    3792..3825
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     regulatory      complement(3826..4233)
                     /label="derived from Nicotiana tabacum psbA"
                     /note="derived from Nicotiana tabacum psbA"
                     /regulatory_class="terminator"
     CDS             complement(4237..5025)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      complement(5026..5200)
                     /note="Prrn; derived from Nicotiana tabacum"
                     /regulatory_class="promoter"
     protein_bind    complement(5201..5234)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     tRNA            complement(5427..5497)
                     /gene="trnG"
                     /product="tRNA-Gly"
                     /label="trnG"
     gene            complement(5427..5497)
                     /gene="trnG"
                     /label="trnG"
     misc_feature    complement(5773..5903)
                     /label="similar to psbZ"
                     /note="similar to psbZ"
     promoter        complement(5917..5935)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5956..5972)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    5980..5996
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6004..6034)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6049..6070)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6358..6946)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7120..7977)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7978..8082)
                     /label="AmpR promoter"
     rep_origin      complement(8108..8563)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8705..8721
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8731..8749
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8761..9961
                     /label="similar to psaB"
                     /note="similar to psaB"
     CDS             10084..10383
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from Nicotiana
                     sylvestris. Accession#: Q3C1I0"
     tRNA            10536..10609
                     /gene="trnfM"
                     /product="tRNA-fMet"
                     /label="trnfM"
     gene            10536..10609
                     /gene="trnfM"
                     /label="trnfM"
ORIGIN
        1 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
       61 aagctggagc tcggtacccc aaagctcccc cgccgtcgtt caatgagaat ggataagagg
      121 ctcgtgggat tgacgtgagg gggcagggat ggctatattt ctgggagcga actccgggcg
      181 aatacgaagc gcttggatac ggatccaaat actgcagttt aactttaaga aggagatata
      241 cccatggata ctctattgag aacccataac aggctagaat tgctataccc acttcatgaa
      301 ttagctaaaa ggcacttcct gtccccttct cccaatcccc agaaccctaa tttcaagttt
      361 ttcagcagga aaccctatca aaaaaagtgt aggaatggat atattggggt aagtagtaat
      421 caacttcttg acttagtgcc tgaaattaag aaagagcacc tcgaattcga tctacctttg
      481 tatgatccat caaaagctct caccttggac cttgcagtag tcggtggggg acccgctggg
      541 ctcgcagttg ctcaacaagt ctcggaggcg ggcctttcgg ttgtttcgat tgacccgaat
      601 ccgaaactca tctggccaaa taattatggt gtttgggttg atgagtttga agacatggac
      661 cttttagatt gccttgatgc tacttggtcc ggtgcgattg tttatgtcga tgatcgtagt
      721 acgaagaacc tcagcaggcc gtatgcacgg gttaatcgta agaatctcaa gtccaagatg
      781 atgaaaaagt gtgtttcgaa tggtgttaga ttccaccaag caacagttgt gaaggcaatg
      841 catgaggagg agaagtcata cttgatttgt agcgatgggg tcactattga cgcgagagtc
      901 gttcttgatg ccaccggctt ttctagatgc ttggttcagt acgataagcc gtacaatccc
      961 gggtatcaag tagcttatgg gattttagct gaagtagaag aacacccttt tgatgttgat
     1021 aaaatggtct tcatggattg gagggattca catctaaatg ggaaggcaga gttgaacgag
     1081 aggaatgcga aaatccccac ttttctttat gcgatgccct tctcttctaa cagaatattt
     1141 ttggaggaga cctccttggt tgctcgtcct ggtttgaaaa tggaagatat acaagagagg
     1201 atggtggctc gattgaatca tttgggtata agaataaaga gtattgaaga agatgagagg
     1261 tgtgtgattc caatgggtgg acctctgccc gtcattcctc aaagggttgt tggaatcggg
     1321 gggactgcag gaatggtgca cccatcaacg ggttacatgg tagcgagaac tcttgcagct
     1381 gcgccaatag ttgctaattc aatcgtacag taccttgttt cagatagcgg tctttcaggt
     1441 aatgatcttt ctgccgatgt ttggaaggat ctttggccta ttgagaggag gaggcaacgg
     1501 gagttcttct gctttggtat ggatatcctt ctcaagcttg atttagaagg aacaagaaga
     1561 ttttttgatg cattttttga tttagaacct cggtattggc atggattttt gtcctcaaga
     1621 ttgtttcttc ctgagctagt gccttttggg ctttctttgt tctctcatgc ttcaaatact
     1681 tgtaagttag agatcatggc taagggtact cttcctttgg tcaacatgat taataactta
     1741 gttcaagata gggactagac cgcggtctag aaattcaatt aaggaaataa attaaggaaa
     1801 tacaaaaagg ggggtagtca tttgtatata actttgtatg acttttctct tctatttttt
     1861 tgtatttcct ccctttcctt ttctatttgt atttttttat cattgcttcc attgaattcg
     1921 atatcaagct taggatcgtt tatttacaac ggaatggtat acaaagtcaa cagatctcaa
     1981 ggcggccgcg aaggagatat acatatgact gctgctgttg ctgaacctag aattgtacct
     2041 cgacaaactt ggattggttt aactttagct ggtatgattg ttgctggatg gggatcttta
     2101 catgtttatg gagtttattt tcatagatgg ggtacttctt ctttagttat tgttcctgct
     2161 attgtagctg ttcaaacttg gttatctgtt ggtttattta ttgttgctca tgatgctatg
     2221 catggttctt tagctcctgg acgacctaga ttaaatgctg ctgtaggacg attaacttta
     2281 ggattatatg ctggatttag atttgatcgt ttaaaaactg ctcatcatgc tcatcatgct
     2341 gctcctggta ctgctgatga tcctgatttt tatgctcctg ctcctagagc ttttttacct
     2401 tggtttttaa atttttttcg aacttatttt ggatggcgag aaatggctgt attaactgct
     2461 ttagttttaa ttgctttatt tggattagga gctagacctg ctaatttatt aactttttgg
     2521 gctgctcctg ctttattatc tgctttacaa ttatttactt ttggtacttg gttacctcat
     2581 agacatactg atcaaccttt tgctgatgct catcatgctc gatcttctgg atatggacct
     2641 gttttatctt tattaacttg ttttcatttt ggtagacatc atgaacatca tttaactcct
     2701 tggagacctt ggtggagatt atggagagga gaatcttaag gatccctaga gtagacatta
     2761 gcagataaat tagcaggaaa taaagaagga taaggagaaa gaactcaagt aattatcctt
     2821 cgttctctta attgaattgc aattaaactc ggcccaatct tttactaaaa ggattgagcc
     2881 gaatacaaca aagattctat tgcatatatt ttgactaagt atatacttac ctagatatac
     2941 aagatttgaa atacaaaatc tagcaagctt atcgatagga tcgtttattt acaacggaat
     3001 ggtatacaaa gtcaacagat ctcaactgca ggggagggat ttccatggct tggttaactt
     3061 ggattgcttt atttttaact gcttttttag gtatggaagc atttgcttgg attatgcata
     3121 gatatgtaat gcatggattt ttatggtctt ggcatagatc tcatcatgaa cctcatgatc
     3181 atcctttaga aaaaaatgat ttatttgctg tagtttttgc tgctcctgct attgttatgg
     3241 ttgctgttgg attacattta tggccttggg ctttacctgt aggtttaggt attactgctt
     3301 atggtatggt atattttttt tttcatgatg gattagtaca tagaagattt cctactggat
     3361 tttctggtcg atctggtttt tggactcgac gtattcaagc tcatcgttta catcatgctg
     3421 ttagaactag agaaggttgt gtttcttttg gttttttatg ggtaagatct gcacgagctt
     3481 taaaagctga attagctcaa aaaagaggtt cttcttcttc tggagcttaa gtcgactttt
     3541 tcatgatgtt tatgtgaata gcataaacat cgtttttatt tttatggtgt ttaggttaaa
     3601 tacctaaaca tcattttaca tttttaaaat taagttctaa agttatcttt tgtttaaatt
     3661 tgcctgtctt tataaattac gatgtgccag aaaaataaaa tcttagcttt ttattataga
     3721 atttatcttt atgtattata ttttataagt aataaaagct cgaggggggg cccggtacta
     3781 actagaattc aataacttcg tatagcatac attatacgaa gttataggtc gaatatagct
     3841 cttctttctt atttcaatga tattattatt tcaaagataa gagatattca aagataagag
     3901 ataagaagaa gtcaaaattt gatttttttt ttggaaaaaa aaaatcaaaa agatatagta
     3961 acattagcaa gaagagaaac aagttctatt tctacaattt taaacaaata caaaatcaaa
     4021 atagaatact caatcatgaa taaatgcaag aaaataacct ctccttcttt ttctataatg
     4081 taaacaaaaa agtctatgta agtaaaatac tagtaaataa ataaaaagaa aaaaagaaag
     4141 gagcaatagc accctcttga tagaacaaga aaatgattat tgctcctttc ttttcaaaac
     4201 ctcctataga ctaggccagg atcgctctag acattatttg ccgactacct tggtgatctc
     4261 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     4321 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     4381 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     4441 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     4501 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caagaaccgg
     4561 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     4621 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     4681 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     4741 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     4801 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     4861 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     4921 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     4981 ctctgatagt tgagtcgata cttcggcgat caccgcttct gccataaatc cctccctaca
     5041 actgtatcca agcgcttcgt attcgcccgg agttcgctcc cagaaatata gccatccctg
     5101 ccccctcacg tcaatcccac gagcctctta tccattctca ttgaacgacg gcgggggagc
     5161 tttgggtacc gatcccaatt cgggatctgt cgattaatta ataacttcgt atagcataca
     5221 ttatacgaag ttatacctag ctagtaatta attcccgcct ttcgcttttt gggggtggaa
     5281 ggaaaaagaa aacgtagggg agggatagaa tcactacact atcacggcca actataccaa
     5341 atccttaatt taaggatata tttaatgcta tttatgaaat taaataataa ataaatagta
     5401 ataaaattac tttatcttgg atcttgggcg gatagcggga atcgaacccg catcttctcc
     5461 ttggcaaaga gaaattttac cattcgacca tatccgcatt tttttgttct tgatacacaa
     5521 tatgtaccca catatatgat atataaccgg atcttttttg tgcagtgccg ggacacatat
     5581 tctcttcgga acgattccaa ttattttttt aattgtattc tttttattca agaagtttga
     5641 cccccctcta atttttttgt tttctttatt tgatttgcgt tttctttggg gacttagatt
     5701 ccaatttaat gtgtctcaca accgagaaaa attagggggg tcattttggt tttgggtctg
     5761 cgacgaatag gttcaagaga tgagagaatt aaggataccc accagaaaga ctaatccaat
     5821 ccataaggat gtaccagaaa atacaacatt tttgttactt gaccagccat caggagaagc
     5881 aaatacaacg ggtacgctaa tcaccagctt ttgttccctt tagtgagggt taatttcgag
     5941 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6001 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6061 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6781 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8101 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8161 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8221 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8281 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8341 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8401 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8461 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8521 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     8581 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8641 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8701 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag
     8761 ccttgctcta gcttctttag gggttattac ttctttggta gctcaacaca tgtactcttt
     8821 acctgcttat gcattcatag cacaagactt tactactcaa gctgcattat atacccacca
     8881 ccaatatatc gcaggattca tcatgacagg agcttttgct catggagcta tatttttcat
     8941 tagagattac aatccggagc aaaatgaaga taatgtattg gcaagaatgt tagagcataa
     9001 agaagctatc atatctcatt taagttgggc cagcctcttt ctgggattcc ataccctggg
     9061 actttatgtt cataatgatg tcatgcttgc ctttggcact ccggagaagc aaatcttgat
     9121 tgaacctata tttgctcaat ggatacaatc cgctcatggt aaaacttcat atgggttcga
     9181 tgtactttta tcttcaacga gtggtccagc attcaatgcg ggtcgaagca tctggttgcc
     9241 gggttggtta aatgctgtta atgaaaatag taattcatta tttttaacaa taggtcctgg
     9301 agactttttg gttcatcatg ctattgctct tggtttacat acaactacat tgatcttagt
     9361 aaaaggtgct ttagatgcac gtggttccaa gttaatgcca gataaaaagg atttcggtta
     9421 tagttttccg tgcgatggcc caggacgagg cggtacttgt gatatttcgg catgggacgc
     9481 gttttatttg gcagtttttt ggatgttaaa tactattgga tgggttactt tttattggca
     9541 ttggaagcac atcacattat ggcagggtaa cgtttcacag tttaatgaat cttccactta
     9601 tttgatgggc tggttaaggg attatttatg gttaaactct tcacaactta tcaacggata
     9661 taatcctttt ggtatgaata gtttatcggt ttgggcatgg atgttcttat ttggacatct
     9721 tgtttgggct actggattta tgttcttaat ttcttggcgt ggatattggc aggaattgat
     9781 tgaaacttta gcatgggctc atgaacgcac acctttggcc aatttgattc gctggagaga
     9841 taaaccagtg gccctttcca ttgtacaagc aagattggtt ggattagctc acttttctgt
     9901 aggttatata ttcacttatg cggctttctt gattgcctct acgtcgggca aatttggtta
     9961 atatttttaa tgtgtgtatc tgcgataatc tcatttcttt cgacggagag ggggtccacc
    10021 ttcttctatt tctacatcta ggattcgact tgtatcatgg atactaatag gaattcaacc
    10081 attatggcaa ggaaaagttt gattcagagg gagaagaaga ggcaaaaatt ggaacagaaa
    10141 tatcattcga ttcgtcgatc ctcaaagaaa gaaataagca aggttccgtc gttgagtgac
    10201 aaatgggaaa tttatggaaa gttacaatcc ccaccacgga atagtgcacc tacacgcctt
    10261 catcgacgtt gttttttgac cggaaggccg agagctaact atcgagactt tggactatcc
    10321 ggacacatac ttcgtgaaat ggttcatgca tgtttgttgc caggagcaac aagatcaagt
    10381 tggtaaggat taacgcttca tttctatttc tatggtcgat gatcatagaa gcccctttac
    10441 cattctgtat aaatgggcta ttctatttgt acagataggg tggaggggcg catttaatcc
    10501 ttgtttatct attagttttc agttcttatc ttcggcgcgg ggtagagcag tttggtagct
    10561 cgcaaggctc ataaccttga ggtcacgggt tcaaatcctg tctccgcaac atcttgtttt
    10621 gccaaactat tttagggtt
//
