LOCUS       V015225                 8495 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015225
VERSION     V015225
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(660..687)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(706..792)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(965..1699)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        2078..2182
                     /label="AmpR promoter"
     CDS             complement(2218..2925)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      complement(4388..4422)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             5171..6028
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6202..6790
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6976..7116)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(7221..7409)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 ggggtgatgt caaagcttga aaaaacgcac gtaacaaaag caaaatttat gctccatggg
       61 ggagactaca accccgatca gtggctggat cggcccgata ttttagctga cgatatcaaa
      121 ctgatgaagc tttctcatac gaatacgttt tctgtcggca tttttgcatg gagcgcactt
      181 gagccggagg agggcgtata tcaatttgaa tggctggatg atatttttga gcggattcac
      241 agtataggcg gccgggtcat attagcaacg ccgagcggag cccgtccggc ctggctgtcg
      301 caaacctatc cggaagtttt gcgcgtcaat gcctcccgcg tcaaacagct gcacggcgga
      361 aggcacaacc actgcctcac atctaaagtc taccgagaaa aaacacggca catcaaccgc
      421 ttattagcag aacgatacgg acatcacccg gcgctgttaa tgtggcacat ttcaaacgaa
      481 tacgggggag attgccactg tgaatcgatg cggccgctct agagcaacgt tcttgccatt
      541 gctgcataaa aaacgcccgg cggcaaccga gcgttctgaa ttaattaatc atcgggaaga
      601 tcttcatcac cgaaacgcgg caggcagctc tagagttaac aagagtttgt agaaacgcaa
      661 aaaggccatc cgtcaggatg gccttctgct tagctagagc ggcggatttg tcctactcag
      721 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      781 tcgttttatt tgatgcctca agctagagag tcattaccag atctcactgc agagatcccc
      841 gggtctagag tctagggacc tctttagctc cttggaagct gtcagtagta tacctaataa
      901 tttatctaca ttccctttag taacgtgtaa ctttccaaat ttacaaaagc gactcataga
      961 attatttcct cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag
     1021 taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag
     1081 taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc
     1141 aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt
     1201 ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag
     1261 tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt
     1321 caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg
     1381 tgcttaaatg gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa
     1441 actgtagaat atcttggtga attaaagtga cacgagtatt cagttttaat ttttctgacg
     1501 ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca
     1561 gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta
     1621 aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat
     1681 attttatatt tttgttcatg taatcactcc ttcttaatta caaattttta gcatctaatt
     1741 taacttcaat tcctattata caaaatttta agatactgca ctatcaacac actcttaagt
     1801 ttgcttctaa gtcttatttc cataacttct tttacgtttc cgccattctt tgctgtttcg
     1861 atttttatga tatggtgcaa gtcagcacga acacgaaccg tcttatctcc cattatatct
     1921 ttttttgcac tgattggtgt atcatttcgt ttttcttttg tgctagagga tcaattcttg
     1981 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2161 ataatattga aaaaggaaga gtgcggccgc ccgcgggagc tcggatcctt aaaactactt
     2221 gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac
     2281 gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc
     2341 gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc
     2401 gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta
     2461 catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga
     2521 ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga
     2581 gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa
     2641 gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc
     2701 cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc
     2761 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
     2821 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
     2881 ctccttgatg atggccatgt tatcctcctc gcccttgctc accatttccc cctttgattt
     2941 ttagatatca ctagtttgga ccatttgtca tttccccctt tgatttaagt gaacaagttt
     3001 atccatcaac tatcttaatt gagttagttt gtttatccaa taaactaact ttatctcatc
     3061 atatacaaaa taaatgttta tttcaatgtt ttttttagaa aatttagtta taatattaga
     3121 tatgatactt ttaaatatct aattcaagct tcaaaaaaca ccaacttagt tcggtggata
     3181 aacaaaggag tggttattat tcaaattgca gatcaagctt tagtaaaaaa aatgaatcaa
     3241 aaattaatat tagatgaaat tttgaagaac tcccctgtct ccagggcaac tctctctgag
     3301 attacaggat taaacaagtc tactgtctcc tctcaagtaa atacactgct tgaaaaagat
     3361 tttatttttg aaattggggc agggcaatct agaggcggca gaagacctgt aatgcttgtt
     3421 tttaataaga atgcaggcta ctcgattggt attgatatag gagtcgacta tcttaacgga
     3481 attctaaccg acttagaagg aaatattatt ctcgagaaga cttctgactt gtctagttct
     3541 tccgctagtg aagtaaaaga gattttattt gcacttattc atggttttgt aacccatatg
     3601 cctgagtccc cttatggtct agtcggaata ggaatttgtg ttccaggcct tgtagatcgt
     3661 catcagcaaa ttattttcat gcctaactta aattggaata tcaaagattt gcagttttta
     3721 attgagagtg agtttaatgt tccggttttt gttgaaaatg aagctaatgc aggagcatac
     3781 ggtgaaaaag tatttggtat gacaaaaaac tatgaaaaca tcgtttacat cagtattaat
     3841 atcggaattg gaactggact tgttattaac aacgaattgt ataaaggtgt tcagggtttt
     3901 tctggggaaa tgggtcatat gacgatagat tttaatggac ccaaatgcag ctgtggaaat
     3961 cgaggctgtt gggaattata tgcttctgaa aaagcgttac tggcttcgct ctctaaagaa
     4021 gaaaagaata tttctcgaaa agagattgtg gaacgcgcaa ataaaaatga tgtagaaatg
     4081 ttaaatgcac ttcaaaactt tggcttttat atcggaattg gattaaccaa tatccttaat
     4141 acatttgata tagaagctgt tatcttgaga aatcatataa ttgaatctca tcccattgtt
     4201 ttaaatacga ttaaaaacga agtttcttct agagtccatt ctcatttaga caataaatgt
     4261 gaactattgc cttcttcgtt aggaaaaaat gcacctgctt taggagcggt ttctatcgtt
     4321 attgattctt ttttaagtgt tacccctata agttaggagc tccccgggac gttcttgcca
     4381 ttgctgcata aaaaacgccc ggcggcaacc gagcgttctg aattaattaa tcatcgcgac
     4441 tgcagagata tcgatttcaa gctatatttg gagttgagcc tcttgaaacg gacaccctgt
     4501 atccgaagga tcgaaacgct gtcagctacc gcagccaaat atatgaaatg aaggattatg
     4561 caaccgtgat tgatgtaaag acagcttcag tggaagcggt gtatcaagaa gatttttatg
     4621 cgcgcacgcc agcggtcaca agccatgagt atcagcaggg caaggcgtat tttatcggcg
     4681 cgcgtttgga ggatcaattt cagcgtgatt tctatgaggg tctgatcaca gacctgtctc
     4741 tctctccagt ttttccggtt cggcacggaa aaggcgtctc cgtacaagcg aggcaggatc
     4801 aggacaatga ttatattttt gtcatgaatt tcacggaaga aaaacagctg gtcacgtttg
     4861 atcagagtgt gaaggacata atgacaggag acatattgtc aggcgacctg acgatggaaa
     4921 agtatgaagt gagaattgtc gtaaacacac attagcccat cattcttgaa gacgaaaggg
     4981 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5041 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5101 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5161 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5221 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5281 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5341 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5401 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5461 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5521 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5581 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5641 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5701 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5761 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5821 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5881 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5941 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6001 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6061 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6121 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     6601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6901 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6961 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7021 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     7081 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     7141 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7201 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     7261 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     7321 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     7381 ttaagggcgg ttttttcctg tttggtcact tgatgcctcc gtgtaagggg gaatttctgt
     7441 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg
     7501 atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc
     7561 gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg
     7621 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
     7681 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     7741 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     7801 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     7861 cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc
     7921 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc
     7981 tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc
     8041 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     8101 gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc
     8161 ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc
     8221 gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     8281 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     8341 gcctcgcgtc gcgactaaga aaatgccgtc aaatccgctc gccatgactt cactaacgat
     8401 gcctttgaaa atcttcaagt tcttttctac taattcaagg cgtgtctcac caggtttttg
     8461 gtttgctccg gcgcaaatgc agacaatatc aggat
//
