LOCUS       V012952                 9860 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012952
VERSION     V012952
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(660..687)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(706..792)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(965..1699)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        2078..2182
                     /label="AmpR promoter"
     CDS             complement(2200..2217)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(2218..4281)
                     /gene="ppk"
                     /label="Polyphosphate kinase"
                     /note="Polyphosphate kinase from Escherichia coli O157:H7.
                     Accession#: P0A7B2"
     terminator      complement(5753..5787)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             6536..7393
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7567..8155
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8341..8481)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(8586..8774)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 ggggtgatgt caaagcttga aaaaacgcac gtaacaaaag caaaatttat gctccatggg
       61 ggagactaca accccgatca gtggctggat cggcccgata ttttagctga cgatatcaaa
      121 ctgatgaagc tttctcatac gaatacgttt tctgtcggca tttttgcatg gagcgcactt
      181 gagccggagg agggcgtata tcaatttgaa tggctggatg atatttttga gcggattcac
      241 agtataggcg gccgggtcat attagcaacg ccgagcggag cccgtccggc ctggctgtcg
      301 caaacctatc cggaagtttt gcgcgtcaat gcctcccgcg tcaaacagct gcacggcgga
      361 aggcacaacc actgcctcac atctaaagtc taccgagaaa aaacacggca catcaaccgc
      421 ttattagcag aacgatacgg acatcacccg gcgctgttaa tgtggcacat ttcaaacgaa
      481 tacgggggag attgccactg tgaatcgatg cggccgctct agagcaacgt tcttgccatt
      541 gctgcataaa aaacgcccgg cggcaaccga gcgttctgaa ttaattaatc atcgggaaga
      601 tcttcatcac cgaaacgcgg caggcagctc tagagttaac aagagtttgt agaaacgcaa
      661 aaaggccatc cgtcaggatg gccttctgct tagctagagc ggcggatttg tcctactcag
      721 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      781 tcgttttatt tgatgcctca agctagagag tcattaccag atctcactgc agagatcccc
      841 gggtctagag tctagggacc tctttagctc cttggaagct gtcagtagta tacctaataa
      901 tttatctaca ttccctttag taacgtgtaa ctttccaaat ttacaaaagc gactcataga
      961 attatttcct cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag
     1021 taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag
     1081 taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc
     1141 aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt
     1201 ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag
     1261 tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt
     1321 caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg
     1381 tgcttaaatg gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa
     1441 actgtagaat atcttggtga attaaagtga cacgagtatt cagttttaat ttttctgacg
     1501 ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca
     1561 gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta
     1621 aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat
     1681 attttatatt tttgttcatg taatcactcc ttcttaatta caaattttta gcatctaatt
     1741 taacttcaat tcctattata caaaatttta agatactgca ctatcaacac actcttaagt
     1801 ttgcttctaa gtcttatttc cataacttct tttacgtttc cgccattctt tgctgtttcg
     1861 atttttatga tatggtgcaa gtcagcacga acacgaaccg tcttatctcc cattatatct
     1921 ttttttgcac tgattggtgt atcatttcgt ttttcttttg tgctagagga tcaattcttg
     1981 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2161 ataatattga aaaaggaaga gtgcggccgc ccgcggttaa tgatggtggt gatgatgttc
     2221 aggttgttca agagatttga tgtaatcgta gatagcaagt tgagcacgaa ctttacgacg
     2281 gttgccacga ggaacgtaac ggttagaaag ttctttatcg atgtaacgag ctttaactgt
     2341 atcagagaaa aggatatcga tgatatcaag aacacgttgt ttaagacgag gatcaagaag
     2401 aggtgtagca acttcgatac ggtaatcgat gttacgtgtc atccaatcag cagaagaaag
     2461 gtaaactttt ttatcgccgc cgttttcgaa gatgtaaaca cgatcatgtt caaggtaacg
     2521 atcaacgata gagatagcac ggatgttatc agagatgcct tcaaggttag ggataagaga
     2581 gcacatgcca cgaacaagaa ggttaacagg aacgccagaa gaagaagcag cgtaaagacg
     2641 atcaacaagg cctttatcaa caaggttgtt aagtttaagt gtgatgccag aaggaaggcc
     2701 ttgttgagcg ttagcgattt cacgatcaac catttcgtaa agaagacgac gagagttttg
     2761 aggagaaacc ataaggtaat cgaatgtaac aggacggtaa gggttttcga tgaagttgaa
     2821 aacacgacga acttcgtttg tgatacgagc atcagctgta agaagagagt aatctgtgta
     2881 aagacgagct gttttttcgt tgaagttgcc tgtgccgata tgagcgtaac gaacaacttc
     2941 gccgttttct ttacgagaga taaggaaaag tttagcatgg attttaaggc caggagcaga
     3001 gaagataaca tgaacgccag cttctgtaag acgtttagcc caatggatgt tagcttcttc
     3061 atcgaaacga gcttgaagtt caacaacaac tgtaactttt ttgccgttat gagcagcatg
     3121 gatcatagaa tcgatgatac gagaatcttt agcaacacgg tagatgttga ttttgatagc
     3181 aagaacagaa ggatcgaaag aagcttgacg aagaagttca agaacatgtt cgaatgtatg
     3241 gtaagggtag taaagaagaa catcacgttc acggatagca tcgaagccgt tacggaattg
     3301 agctttatcg aaccagatat gacgaagacg aggaagaggt ttgttaacaa ggttagcttt
     3361 gccaacgtta gggaagttga tgaaatcttt gaagttatgg taacggccgc caggaacgat
     3421 agaatcgtaa cgagagattg taagtttttc acgaagaact tcaacaagag cgttaggcat
     3481 atcacgttgg taaacgaaac gaacaggttc agctgtaaga cgttgtttaa gagaagaaga
     3541 cataagttcc ataagagaag cttccatttc atgaacaaga tcgtattcag catcacgtgt
     3601 cattttcata gagtaagcgt taagagcatc gtaatcgaag aagcctttga agatatcatc
     3661 aaggcagtaa cgaaggatgt tatcaagaag gatcataggt ttacgacgac gaggagcttc
     3721 aggaggaagg ttaacgaaac gaggaacttt atcagaaggg atttcaagaa gagcgtaacg
     3781 gattgtatcg ccacggatga tttcaacagc aaggtatgtg taatcatctt taaggaattg
     3841 aacaagatct gtatcagggt tgataaggat aggtgtgata tgttgacgaa ggtattgttt
     3901 gaagtaatga cgaagccagt tttgttggtt aacagaaagt tgacgttcgt tgataaggaa
     3961 gatttggtta cgagccattt caagaagaag ttcgttgtaa aggccatcga attcttgatc
     4021 agctttaaga acacgagatt ggattttgcc aagaagatga cgagaatgag agttagagcc
     4081 ttgttcttca gagatgatga tacgacgttt aagttcagcg aaacgaactt tgtagaattc
     4141 atcaaggttg ttagagtaga tgccaaggaa acgcatacgt tcgataagag ggttagattt
     4201 atcagcagct tcttgaagaa cacgttcgtt gaaagaaagc caagaaagtt ctttttcgat
     4261 gtaaagtttt tcttggccca tggatcccat ttcccccttt gatttttaga tatcactagt
     4321 ttggaccatt tgtcatttcc ccctttgatt taagtgaaca agtttatcca tcaactatct
     4381 taattgagtt agtttgttta tccaataaac taactttatc tcatcatata caaaataaat
     4441 gtttatttca atgttttttt tagaaaattt agttataata ttagatatga tacttttaaa
     4501 tatctaattc aagcttcaaa aaacaccaac ttagttcggt ggataaacaa aggagtggtt
     4561 attattcaaa ttgcagatca agctttagta aaaaaaatga atcaaaaatt aatattagat
     4621 gaaattttga agaactcccc tgtctccagg gcaactctct ctgagattac aggattaaac
     4681 aagtctactg tctcctctca agtaaataca ctgcttgaaa aagattttat ttttgaaatt
     4741 ggggcagggc aatctagagg cggcagaaga cctgtaatgc ttgtttttaa taagaatgca
     4801 ggctactcga ttggtattga tataggagtc gactatctta acggaattct aaccgactta
     4861 gaaggaaata ttattctcga gaagacttct gacttgtcta gttcttccgc tagtgaagta
     4921 aaagagattt tatttgcact tattcatggt tttgtaaccc atatgcctga gtccccttat
     4981 ggtctagtcg gaataggaat ttgtgttcca ggccttgtag atcgtcatca gcaaattatt
     5041 ttcatgccta acttaaattg gaatatcaaa gatttgcagt ttttaattga gagtgagttt
     5101 aatgttccgg tttttgttga aaatgaagct aatgcaggag catacggtga aaaagtattt
     5161 ggtatgacaa aaaactatga aaacatcgtt tacatcagta ttaatatcgg aattggaact
     5221 ggacttgtta ttaacaacga attgtataaa ggtgttcagg gtttttctgg ggaaatgggt
     5281 catatgacga tagattttaa tggacccaaa tgcagctgtg gaaatcgagg ctgttgggaa
     5341 ttatatgctt ctgaaaaagc gttactggct tcgctctcta aagaagaaaa gaatatttct
     5401 cgaaaagaga ttgtggaacg cgcaaataaa aatgatgtag aaatgttaaa tgcacttcaa
     5461 aactttggct tttatatcgg aattggatta accaatatcc ttaatacatt tgatatagaa
     5521 gctgttatct tgagaaatca tataattgaa tctcatccca ttgttttaaa tacgattaaa
     5581 aacgaagttt cttctagagt ccattctcat ttagacaata aatgtgaact attgccttct
     5641 tcgttaggaa aaaatgcacc tgctttagga gcggtttcta tcgttattga ttctttttta
     5701 agtgttaccc ctataagtta ggagctcccc gggacgttct tgccattgct gcataaaaaa
     5761 cgcccggcgg caaccgagcg ttctgaatta attaatcatc gcgactgcag agatatcgat
     5821 ttcaagctat atttggagtt gagcctcttg aaacggacac cctgtatccg aaggatcgaa
     5881 acgctgtcag ctaccgcagc caaatatatg aaatgaagga ttatgcaacc gtgattgatg
     5941 taaagacagc ttcagtggaa gcggtgtatc aagaagattt ttatgcgcgc acgccagcgg
     6001 tcacaagcca tgagtatcag cagggcaagg cgtattttat cggcgcgcgt ttggaggatc
     6061 aatttcagcg tgatttctat gagggtctga tcacagacct gtctctctct ccagtttttc
     6121 cggttcggca cggaaaaggc gtctccgtac aagcgaggca ggatcaggac aatgattata
     6181 tttttgtcat gaatttcacg gaagaaaaac agctggtcac gtttgatcag agtgtgaagg
     6241 acataatgac aggagacata ttgtcaggcg acctgacgat ggaaaagtat gaagtgagaa
     6301 ttgtcgtaaa cacacattag cccatcattc ttgaagacga aagggcctcg tgatacgcct
     6361 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     6421 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6481 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6541 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6601 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6661 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6721 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     6781 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6841 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6901 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6961 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7021 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     7081 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7141 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7201 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7261 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7321 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7381 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7441 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7501 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7561 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7621 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7681 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     7741 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7801 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7861 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7921 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     7981 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8041 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8101 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8161 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8221 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8281 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8341 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     8401 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     8461 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8521 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8581 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     8641 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     8701 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     8761 tcctgtttgg tcacttgatg cctccgtgta agggggaatt tctgttcatg ggggtaatga
     8821 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     8881 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     8941 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     9001 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     9061 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     9121 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     9181 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     9241 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     9301 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     9361 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
     9421 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
     9481 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
     9541 gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
     9601 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     9661 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgac
     9721 taagaaaatg ccgtcaaatc cgctcgccat gacttcacta acgatgcctt tgaaaatctt
     9781 caagttcttt tctactaatt caaggcgtgt ctcaccaggt ttttggtttg ctccggcgca
     9841 aatgcagaca atatcaggat
//
