LOCUS       V013460                10967 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013460
VERSION     V013460
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(546..580)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(660..687)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(706..792)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(965..1699)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        2078..2182
                     /label="AmpR promoter"
     CDS             complement(4672..5388)
                     /label="ECFP"
                     /note="enhanced CFP"
     terminator      complement(6860..6894)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             7643..8500
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8674..9262
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(9448..9588)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(9693..9881)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 ggggtgatgt caaagcttga aaaaacgcac gtaacaaaag caaaatttat gctccatggg
       61 ggagactaca accccgatca gtggctggat cggcccgata ttttagctga cgatatcaaa
      121 ctgatgaagc tttctcatac gaatacgttt tctgtcggca tttttgcatg gagcgcactt
      181 gagccggagg agggcgtata tcaatttgaa tggctggatg atatttttga gcggattcac
      241 agtataggcg gccgggtcat attagcaacg ccgagcggag cccgtccggc ctggctgtcg
      301 caaacctatc cggaagtttt gcgcgtcaat gcctcccgcg tcaaacagct gcacggcgga
      361 aggcacaacc actgcctcac atctaaagtc taccgagaaa aaacacggca catcaaccgc
      421 ttattagcag aacgatacgg acatcacccg gcgctgttaa tgtggcacat ttcaaacgaa
      481 tacgggggag attgccactg tgaatcgatg cggccgctct agagcaacgt tcttgccatt
      541 gctgcataaa aaacgcccgg cggcaaccga gcgttctgaa ttaattaatc atcgggaaga
      601 tcttcatcac cgaaacgcgg caggcagctc tagagttaac aagagtttgt agaaacgcaa
      661 aaaggccatc cgtcaggatg gccttctgct tagctagagc ggcggatttg tcctactcag
      721 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      781 tcgttttatt tgatgcctca agctagagag tcattaccag atctcactgc agagatcccc
      841 gggtctagag tctagggacc tctttagctc cttggaagct gtcagtagta tacctaataa
      901 tttatctaca ttccctttag taacgtgtaa ctttccaaat ttacaaaagc gactcataga
      961 attatttcct cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag
     1021 taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag
     1081 taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc
     1141 aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt
     1201 ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag
     1261 tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt
     1321 caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg
     1381 tgcttaaatg gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa
     1441 actgtagaat atcttggtga attaaagtga cacgagtatt cagttttaat ttttctgacg
     1501 ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca
     1561 gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta
     1621 aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat
     1681 attttatatt tttgttcatg taatcactcc ttcttaatta caaattttta gcatctaatt
     1741 taacttcaat tcctattata caaaatttta agatactgca ctatcaacac actcttaagt
     1801 ttgcttctaa gtcttatttc cataacttct tttacgtttc cgccattctt tgctgtttcg
     1861 atttttatga tatggtgcaa gtcagcacga acacgaaccg tcttatctcc cattatatct
     1921 ttttttgcac tgattggtgt atcatttcgt ttttcttttg tgctagagga tcaattcttg
     1981 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2161 ataatattga aaaaggaaga gtgcggccgc ccgcggtttg tacaattcat tcataccctc
     2221 ggtaatacca gcagcagtca aaaattctaa caagaccatg tggtctctct tttcgtttgg
     2281 atctttgaat aaggcagatt gataggataa gtaatggttg tctggtaaca agactggacc
     2341 atcaccaatt ggagtatttt gttgataatg gtcagctaat tgaacaccac catcttcaat
     2401 gttgtgtcta attttgaagt tagctttgat accattcttt tgtttgtcag cagtgatgta
     2461 aacattgtga gagttatagt tgtattccaa tttgtgacct aaaatgttac catcttcttt
     2521 aaaatcaata ccttttaatt cgattctatt aactaaggta tcaccttcaa acttgacttc
     2581 agctctggtc ttgtagttac cgtcatcttt gaaaaaaata gttctttctt gaacataacc
     2641 ttctggcatg gcagacttga aaaagtcatg ttgtttcata tgatctgggt atctagcaaa
     2701 acattgaaca ccataaccta aagtagtgac taaggttggc catggaactg gcaatttacc
     2761 agtagtacag agtaatttta aggtcaattt accgtacgta gcatcacctt caccttcacc
     2821 ggagacagaa aatttgtgac cattaacatc accatctaat tcaaccaaaa ttgggacaac
     2881 accagtgaat aattcttcac ctttagacat ggttccaccg ctgcctccaa agagtttctg
     2941 agacaggaaa cggaatgtct tgatgaccaa ggtctggtca gctgggggag accctccaat
     3001 ccacttgtcc gttttggacc aggaaagatc atccttggtg ctgtcgtagt agccgatctt
     3061 cttgtagctg ccgccctgta gctgctcgat aagtgtccat gccatccggg agccgctggc
     3121 atcaaagacc acatggccag aaacgccctc aaaggaggag gagttcatgg cccggtagat
     3181 ctggtctgta atggtctggt tgttgtagtt aaagtcctcc aggcgcacgc cggaacgacc
     3241 acctcctcca gacgtcttgt tcaaggccaa agccaaggcc cagatagcat cataggccag
     3301 tggtgcctcc tggaagcctc cagtctcctc ggggtgtctt ttcagccgct tggttagttt
     3361 ctccacaaat tcctgtgacg tcatgttgga aatgcttcgg gtgttggcag ggttcagcat
     3421 gacaatctcc gtggtgatgt ggccctccac cgcctcggtc atttcttcca ctgtacaatt
     3481 gattgacggg tcataggtct tgaaccagtt gtcagcatac cacccgatga ggaaccagac
     3541 gtacttcttc ccaaagagcc tttccttata gacctcacaa aaaactttcc gggcttccgt
     3601 ctcatagaaa agtcccacga tgattcgagc atcttgacgc ttcaggtttt taacaggcac
     3661 agctggatcc gagaagaaac tctgtcggaa agtgatctcg atcccagcct ctttcactcg
     3721 ctcctccagg tcatccagcg ttgaggtgaa gacctcggtg gtctgttgga tggtagcgat
     3781 cttcttccag ccccactttt cgaagagttt cacccgggtg ggattgtgga gtgtggcgga
     3841 tggatgcgtc cggaagaacg tgggaaaccg ctgtcggttt gacaaggctg gtgaactgga
     3901 gccatatgag agcacaataa ggttccacat ccgggcagcc tcagctacaa gtgtggagac
     3961 agaactacag ccaggcatga gaatgatctt gatggggtca ttgtagagta gttcgtacaa
     4021 gtacttggtg gcttgccctg ggtcacactt gctgtcgtgg tggataagct tgagctcgta
     4081 gtccggcagg atgtctctgc ggctgttaac gtcctccagc gccatctcca ccgcgggctg
     4141 gcaggcctgg ccccccggcc agcccccgct catgggaaac agcgccccga tgtatactgc
     4201 acgccgttct gagtgtggcg ttcgattcac ctggcagtgg ggcttggggg tgctccactg
     4261 gccctgacta cagacgctcc gggagctgcc caccagatgg aagtcggggt cacatcggaa
     4321 ctccacccgg gctccatcca gagctgggag gtccccaccc gtcaggaaaa ccttcccatt
     4381 ttccagggtc aaataagact tggagcagat tcggacacag cggctgggtg tgtccatatc
     4441 cgtccaggag ccgttggcca ggcatttgcg caccttgggc cccaccacct cgcgctcccc
     4501 tcggcacaca tattcgatct catagtccac aggcaggaag ttgatggcct tcacctggtc
     4561 gcgagtcaag ccacggtacc tgatgccacc ttcccagggc ggatgtataa tctggcaacc
     4621 ttccgaggtg gcgttggggg tctgcgcccc gccagtaccg cctgatccac ccttgtacag
     4681 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     4741 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     4801 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     4861 gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
     4921 gtcggcggtg atatagacgt tgtggctgat gtagttgtac tccagcttgt gccccaggat
     4981 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     5041 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     5101 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     5161 ggggtagcgg ctgaagcact gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg
     5221 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     5281 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     5341 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg atcccatttc
     5401 cccctttgat ttttagatat cactagtttg gaccatttgt catttccccc tttgatttaa
     5461 gtgaacaagt ttatccatca actatcttaa ttgagttagt ttgtttatcc aataaactaa
     5521 ctttatctca tcatatacaa aataaatgtt tatttcaatg ttttttttag aaaatttagt
     5581 tataatatta gatatgatac ttttaaatat ctaattcaag cttcaaaaaa caccaactta
     5641 gttcggtgga taaacaaagg agtggttatt attcaaattg cagatcaagc tttagtaaaa
     5701 aaaatgaatc aaaaattaat attagatgaa attttgaaga actcccctgt ctccagggca
     5761 actctctctg agattacagg attaaacaag tctactgtct cctctcaagt aaatacactg
     5821 cttgaaaaag attttatttt tgaaattggg gcagggcaat ctagaggcgg cagaagacct
     5881 gtaatgcttg tttttaataa gaatgcaggc tactcgattg gtattgatat aggagtcgac
     5941 tatcttaacg gaattctaac cgacttagaa ggaaatatta ttctcgagaa gacttctgac
     6001 ttgtctagtt cttccgctag tgaagtaaaa gagattttat ttgcacttat tcatggtttt
     6061 gtaacccata tgcctgagtc cccttatggt ctagtcggaa taggaatttg tgttccaggc
     6121 cttgtagatc gtcatcagca aattattttc atgcctaact taaattggaa tatcaaagat
     6181 ttgcagtttt taattgagag tgagtttaat gttccggttt ttgttgaaaa tgaagctaat
     6241 gcaggagcat acggtgaaaa agtatttggt atgacaaaaa actatgaaaa catcgtttac
     6301 atcagtatta atatcggaat tggaactgga cttgttatta acaacgaatt gtataaaggt
     6361 gttcagggtt tttctgggga aatgggtcat atgacgatag attttaatgg acccaaatgc
     6421 agctgtggaa atcgaggctg ttgggaatta tatgcttctg aaaaagcgtt actggcttcg
     6481 ctctctaaag aagaaaagaa tatttctcga aaagagattg tggaacgcgc aaataaaaat
     6541 gatgtagaaa tgttaaatgc acttcaaaac tttggctttt atatcggaat tggattaacc
     6601 aatatcctta atacatttga tatagaagct gttatcttga gaaatcatat aattgaatct
     6661 catcccattg ttttaaatac gattaaaaac gaagtttctt ctagagtcca ttctcattta
     6721 gacaataaat gtgaactatt gccttcttcg ttaggaaaaa atgcacctgc tttaggagcg
     6781 gtttctatcg ttattgattc ttttttaagt gttaccccta taagttagga gctccccggg
     6841 acgttcttgc cattgctgca taaaaaacgc ccggcggcaa ccgagcgttc tgaattaatt
     6901 aatcatcgcg actgcagaga tatcgatttc aagctatatt tggagttgag cctcttgaaa
     6961 cggacaccct gtatccgaag gatcgaaacg ctgtcagcta ccgcagccaa atatatgaaa
     7021 tgaaggatta tgcaaccgtg attgatgtaa agacagcttc agtggaagcg gtgtatcaag
     7081 aagattttta tgcgcgcacg ccagcggtca caagccatga gtatcagcag ggcaaggcgt
     7141 attttatcgg cgcgcgtttg gaggatcaat ttcagcgtga tttctatgag ggtctgatca
     7201 cagacctgtc tctctctcca gtttttccgg ttcggcacgg aaaaggcgtc tccgtacaag
     7261 cgaggcagga tcaggacaat gattatattt ttgtcatgaa tttcacggaa gaaaaacagc
     7321 tggtcacgtt tgatcagagt gtgaaggaca taatgacagg agacatattg tcaggcgacc
     7381 tgacgatgga aaagtatgaa gtgagaattg tcgtaaacac acattagccc atcattcttg
     7441 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     7501 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7561 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7621 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7681 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7741 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7801 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7861 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     7921 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7981 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8041 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8101 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8161 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     8221 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8281 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8341 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8401 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8461 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8521 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8581 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8641 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8701 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8761 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8821 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8881 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8941 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9001 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9061 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9361 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9421 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     9481 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9541 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     9601 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     9661 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     9721 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     9781 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     9841 aagcgggcca tgttaagggc ggttttttcc tgtttggtca cttgatgcct ccgtgtaagg
     9901 gggaatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     9961 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
    10021 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
    10081 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
    10141 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
    10201 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
    10261 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
    10321 acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc
    10381 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
    10441 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
    10501 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
    10561 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt
    10621 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct
    10681 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
    10741 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
    10801 gaaggccatc cagcctcgcg tcgcgactaa gaaaatgccg tcaaatccgc tcgccatgac
    10861 ttcactaacg atgcctttga aaatcttcaa gttcttttct actaattcaa ggcgtgtctc
    10921 accaggtttt tggtttgctc cggcgcaaat gcagacaata tcaggat
//
