LOCUS       V013459                10577 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013459
VERSION     V013459
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(660..687)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(706..792)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(965..1699)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        2078..2182
                     /label="AmpR promoter"
     CDS             complement(4282..4998)
                     /label="ECFP"
                     /note="enhanced CFP"
     terminator      complement(6470..6504)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             7253..8110
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8284..8872
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(9058..9198)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(9303..9491)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 ggggtgatgt caaagcttga aaaaacgcac gtaacaaaag caaaatttat gctccatggg
       61 ggagactaca accccgatca gtggctggat cggcccgata ttttagctga cgatatcaaa
      121 ctgatgaagc tttctcatac gaatacgttt tctgtcggca tttttgcatg gagcgcactt
      181 gagccggagg agggcgtata tcaatttgaa tggctggatg atatttttga gcggattcac
      241 agtataggcg gccgggtcat attagcaacg ccgagcggag cccgtccggc ctggctgtcg
      301 caaacctatc cggaagtttt gcgcgtcaat gcctcccgcg tcaaacagct gcacggcgga
      361 aggcacaacc actgcctcac atctaaagtc taccgagaaa aaacacggca catcaaccgc
      421 ttattagcag aacgatacgg acatcacccg gcgctgttaa tgtggcacat ttcaaacgaa
      481 tacgggggag attgccactg tgaatcgatg cggccgctct agagcaacgt tcttgccatt
      541 gctgcataaa aaacgcccgg cggcaaccga gcgttctgaa ttaattaatc atcgggaaga
      601 tcttcatcac cgaaacgcgg caggcagctc tagagttaac aagagtttgt agaaacgcaa
      661 aaaggccatc cgtcaggatg gccttctgct tagctagagc ggcggatttg tcctactcag
      721 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      781 tcgttttatt tgatgcctca agctagagag tcattaccag atctcactgc agagatcccc
      841 gggtctagag tctagggacc tctttagctc cttggaagct gtcagtagta tacctaataa
      901 tttatctaca ttccctttag taacgtgtaa ctttccaaat ttacaaaagc gactcataga
      961 attatttcct cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag
     1021 taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag
     1081 taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc
     1141 aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt
     1201 ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag
     1261 tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt
     1321 caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg
     1381 tgcttaaatg gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa
     1441 actgtagaat atcttggtga attaaagtga cacgagtatt cagttttaat ttttctgacg
     1501 ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca
     1561 gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta
     1621 aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat
     1681 attttatatt tttgttcatg taatcactcc ttcttaatta caaattttta gcatctaatt
     1741 taacttcaat tcctattata caaaatttta agatactgca ctatcaacac actcttaagt
     1801 ttgcttctaa gtcttatttc cataacttct tttacgtttc cgccattctt tgctgtttcg
     1861 atttttatga tatggtgcaa gtcagcacga acacgaaccg tcttatctcc cattatatct
     1921 ttttttgcac tgattggtgt atcatttcgt ttttcttttg tgctagagga tcaattcttg
     1981 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2161 ataatattga aaaaggaaga gtgcggccgc ccgcggtttg tacaattcat tcataccctc
     2221 ggtaatacca gcagcagtca aaaattctaa caagaccatg tggtctctct tttcgtttgg
     2281 atctttgaat aaggcagatt gataggataa gtaatggttg tctggtaaca agactggacc
     2341 atcaccaatt ggagtatttt gttgataatg gtcagctaat tgaacaccac catcttcaat
     2401 gttgtgtcta attttgaagt tagctttgat accattcttt tgtttgtcag cagtgatgta
     2461 aacattgtga gagttatagt tgtattccaa tttgtgacct aaaatgttac catcttcttt
     2521 aaaatcaata ccttttaatt cgattctatt aactaaggta tcaccttcaa acttgacttc
     2581 agctctggtc ttgtagttac cgtcatcttt gaaaaaaata gttctttctt gaacataacc
     2641 ttctggcatg gcagacttga aaaagtcatg ttgtttcata tgatctgggt atctagcaaa
     2701 acattgaaca ccataaccta aagtagtgac taaggttggc catggaactg gcaatttacc
     2761 agtagtacag agtaatttta aggtcaattt accgtacgta gcatcacctt caccttcacc
     2821 ggagacagaa aatttgtgac cattaacatc accatctaat tcaaccaaaa ttgggacaac
     2881 accagtgaat aattcttcac ctttagacat ggttccaccg ctgcctccga gtttctgtga
     2941 caggaaacgg aatgtcttga tgaccaaggt ctggtcggct gggggagacc ctccgatcca
     3001 cttgtctgtt ttggaccagg aaagatcatc cttggtgctg tcgtagtagc cgatcttctt
     3061 gtagctgccg ccctgtagct gctcgataag cgtccatgcc atccgggagc cgctggcatc
     3121 aaagaccacg tggccagaaa caccctcaaa ggaggaggag ttcatggccc ggtagatttg
     3181 gtctgtaatg gtctggttgt tgtagttaaa gtcctccagg cgcactcctg aacggccacc
     3241 tcctccagag gtcttgttca aggccaaagc caaggcccag atagcatcat aggccagtgg
     3301 tgcctcctgg aagcctccag tctcctcagg gtgtcttttc agccgcttgg ttagtttctc
     3361 cacaaattcc tgtgatgtca tgttggaaat gcttcgggtg ttggcagggt tcagcatgac
     3421 aatctccgtg gtgatatggc cctccaccgc ctcagtcatc tcttctactg tacaattgat
     3481 tgacgggtca taggttttga accagttgtc agcataccac ccgatgagaa accagacata
     3541 cttcttccca aagagccgtt ccttatagac ctcacaaaaa actttccggg cttcggtctc
     3601 atagaaaagt cccacgatga ttcgagcatc ttgacgcttc aggtttttaa caggcacagc
     3661 tggatctgag aagaaactct gtcgaaaagt gatctcaatc ccagcctctt tcactcgctc
     3721 ctccaggtca tccagtgttg aggtgaagac ctcggtagtc tgctggatgg tggcaatctt
     3781 cttccagccc cacttttcga agagtttcac ccgggtggga ttgtggagtg tggcggatgg
     3841 atgtgtccga aagaacgttg gaaaccgctg tcggtttgac aaggctggtg agctggagcc
     3901 atatgagagc acaataaggt tccacatccg ggcagcctcg gctaccagtg tggacacaga
     3961 gctgcagccg ggcatgagga tgatcttgat ggggtcgttg tagagtagtt catacaagta
     4021 cttggtggct tgccctgggt cgcacttgct gtcgtggtgg ataagcttga gctcgtagtc
     4081 cggcaggatg tctctgcggc tgttaacgtc ctccagcgcc atctccaccg caggctggca
     4141 ggcctggccc cccggccagc ccccgctcat gggaaacagc gccccgatgt atactgcacg
     4201 ccgttctgag gaggggtgcg gggggaccct cgggtgaggc cgcgggagat gaggggagtg
     4261 agaagtaccg cctgatccac ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
     4321 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     4381 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     4441 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     4501 gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgat
     4561 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     4621 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     4681 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     4741 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgcccca
     4801 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     4861 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     4921 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     4981 ctcgcccttg ctcaccatgg atcccatttc cccctttgat ttttagatat cactagtttg
     5041 gaccatttgt catttccccc tttgatttaa gtgaacaagt ttatccatca actatcttaa
     5101 ttgagttagt ttgtttatcc aataaactaa ctttatctca tcatatacaa aataaatgtt
     5161 tatttcaatg ttttttttag aaaatttagt tataatatta gatatgatac ttttaaatat
     5221 ctaattcaag cttcaaaaaa caccaactta gttcggtgga taaacaaagg agtggttatt
     5281 attcaaattg cagatcaagc tttagtaaaa aaaatgaatc aaaaattaat attagatgaa
     5341 attttgaaga actcccctgt ctccagggca actctctctg agattacagg attaaacaag
     5401 tctactgtct cctctcaagt aaatacactg cttgaaaaag attttatttt tgaaattggg
     5461 gcagggcaat ctagaggcgg cagaagacct gtaatgcttg tttttaataa gaatgcaggc
     5521 tactcgattg gtattgatat aggagtcgac tatcttaacg gaattctaac cgacttagaa
     5581 ggaaatatta ttctcgagaa gacttctgac ttgtctagtt cttccgctag tgaagtaaaa
     5641 gagattttat ttgcacttat tcatggtttt gtaacccata tgcctgagtc cccttatggt
     5701 ctagtcggaa taggaatttg tgttccaggc cttgtagatc gtcatcagca aattattttc
     5761 atgcctaact taaattggaa tatcaaagat ttgcagtttt taattgagag tgagtttaat
     5821 gttccggttt ttgttgaaaa tgaagctaat gcaggagcat acggtgaaaa agtatttggt
     5881 atgacaaaaa actatgaaaa catcgtttac atcagtatta atatcggaat tggaactgga
     5941 cttgttatta acaacgaatt gtataaaggt gttcagggtt tttctgggga aatgggtcat
     6001 atgacgatag attttaatgg acccaaatgc agctgtggaa atcgaggctg ttgggaatta
     6061 tatgcttctg aaaaagcgtt actggcttcg ctctctaaag aagaaaagaa tatttctcga
     6121 aaagagattg tggaacgcgc aaataaaaat gatgtagaaa tgttaaatgc acttcaaaac
     6181 tttggctttt atatcggaat tggattaacc aatatcctta atacatttga tatagaagct
     6241 gttatcttga gaaatcatat aattgaatct catcccattg ttttaaatac gattaaaaac
     6301 gaagtttctt ctagagtcca ttctcattta gacaataaat gtgaactatt gccttcttcg
     6361 ttaggaaaaa atgcacctgc tttaggagcg gtttctatcg ttattgattc ttttttaagt
     6421 gttaccccta taagttagga gctccccggg acgttcttgc cattgctgca taaaaaacgc
     6481 ccggcggcaa ccgagcgttc tgaattaatt aatcatcgcg actgcagaga tatcgatttc
     6541 aagctatatt tggagttgag cctcttgaaa cggacaccct gtatccgaag gatcgaaacg
     6601 ctgtcagcta ccgcagccaa atatatgaaa tgaaggatta tgcaaccgtg attgatgtaa
     6661 agacagcttc agtggaagcg gtgtatcaag aagattttta tgcgcgcacg ccagcggtca
     6721 caagccatga gtatcagcag ggcaaggcgt attttatcgg cgcgcgtttg gaggatcaat
     6781 ttcagcgtga tttctatgag ggtctgatca cagacctgtc tctctctcca gtttttccgg
     6841 ttcggcacgg aaaaggcgtc tccgtacaag cgaggcagga tcaggacaat gattatattt
     6901 ttgtcatgaa tttcacggaa gaaaaacagc tggtcacgtt tgatcagagt gtgaaggaca
     6961 taatgacagg agacatattg tcaggcgacc tgacgatgga aaagtatgaa gtgagaattg
     7021 tcgtaaacac acattagccc atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     7081 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     7141 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7201 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7261 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7321 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7381 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7441 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     7501 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7561 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7621 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7681 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7741 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     7801 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7861 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7921 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7981 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8041 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8101 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8161 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8221 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8281 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8341 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8401 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     8461 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8521 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8581 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8641 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     8701 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8761 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8821 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8881 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8941 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9001 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     9061 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     9121 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     9181 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     9241 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     9301 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     9361 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     9421 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     9481 tgtttggtca cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac
     9541 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     9601 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     9661 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
     9721 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
     9781 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
     9841 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     9901 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     9961 gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga
    10021 tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
    10081 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga
    10141 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc
    10201 gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac
    10261 gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg
    10321 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
    10381 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgactaa
    10441 gaaaatgccg tcaaatccgc tcgccatgac ttcactaacg atgcctttga aaatcttcaa
    10501 gttcttttct actaattcaa ggcgtgtctc accaggtttt tggtttgctc cggcgcaaat
    10561 gcagacaata tcaggat
//
