LOCUS       40924_5099        4780 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAVA319, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   von Arnim AG, Deng XW, Stacey MG.
  TITLE     Cloning vectors for the expression of green fluorescent protein 
            fusion proteins in transgenic plants
  JOURNAL   Gene 221 (1), 35-43 (1998)
  PUBMED    9852947
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   von Arnim AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-1998) Botany, The University of Tennessee, HBB437,
            Knoxville, TN 37996-1100, USA
REFERENCE   3  (bases 1 to 4780)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1998"; volume: "221"; issue: "1"; pages: "35-43"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-JUL-1998) Botany, The University of Tennessee, HBB437, 
            Knoxville, TN 37996-1100, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        789..1460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1460..1601
                     /note="translational enhancer; 5'UTR from tobacco etch
                     virus"
     CDS             1615..2313
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     regulatory      2323..2533
                     /label=cauliflower mosaic virus 35S terminator
                     /note="cauliflower mosaic virus 35S terminator"
                     /regulatory_class="terminator"
     misc_feature    2534..2576
                     /label=polylinker
                     /note="polylinker"
     promoter        complement(2595..2613)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2634..2650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2658..2674)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2682..2712)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2727..2748)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3036..3624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3798..4655)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4656..4760)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca ggtcaacatg
      721 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
      781 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
      841 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
      901 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
      961 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1021 aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat
     1081 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     1141 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     1201 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     1261 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     1321 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     1381 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     1441 tcatttcatt tggagaggac ctcgagaatt ctcaacacaa catatacaaa acaaacgaat
     1501 ctcaagcaat caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa
     1561 aagcaatttt ctgaaaattt tcaccattta cgaacgatag ccatgggtaa aggagaactt
     1621 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     1681 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1741 tgcactactg gaaaactacc tgttccctgg ccaacacttg tcactacttt ctcttatggt
     1801 gttcaatgct tttcaagata cccagatcat atgaagcggc acgacttctt caagagcgcc
     1861 atgcctgagg gatacgtgca ggagaggacc atcttcttca aggacgacgg gaactacaag
     1921 acacgtgctg aagtcaagtt tgagggagac accctcgtca acaggatcga gcttaaggga
     1981 atcgatttca aggaggacgg aaacatcctc ggccacaagt tggaatacaa ctacaactcc
     2041 cacaacgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     2101 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     2161 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     2221 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     2281 gggattacac atggcatgga tgaactatac agatctatct agagtccgca aaaatcacca
     2341 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     2401 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     2461 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     2521 caaaatccag tgacctgcag cccgggggat ccactagagc ggccgccacc gcggtggagc
     2581 tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     2641 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2701 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2761 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2821 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2881 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2941 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3001 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3061 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3541 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3601 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3661 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3721 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3781 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3841 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3901 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3961 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4021 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4081 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4141 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4201 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4261 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4321 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4381 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4441 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4501 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4561 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4621 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4681 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4741 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
//
