LOCUS       40924_5079        3327 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAV001, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   White KP, Victorsen A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2013) Human Genetics, The University of Chicago, 
            5801 South Ellis Avenue, Chicago, IL 60637, USA
REFERENCE   2  (bases 1 to 3327)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3327)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2013) Human Genetics, The University of Chicago, 5801 South 
            Ellis Avenue, Chicago, IL 60637, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..36
                     /label=forward recombineering primer binding site
                     /note="forward recombineering primer binding site"
     CDS             37..753
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     CDS             778..795
                     /label=tetracysteine tag
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             817..840
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             841..861
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             904..927
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    939..1524
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      1527..1532
                     /gene="aphA"
                     /regulatory_class="minus_35_signal"
     regulatory      1546..1547
                     /gene="aphA"
                     /regulatory_class="minus_10_signal"
     CDS             1606..2406
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(2623..3011)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            3171..3280
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 ggaggttccg gtggaagcgg aggtagcggc ggcagcatgg tgtccaaggg cgaggagctg
       61 ttcaccggcg tggtgcccat cctggtggag ctggatggcg acgtgaacgg ccacaagttc
      121 agcgtgcgcg gcgagggcga gggcgacgcc accaacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg tgaccaccct gacctacggc
      241 gtgcagtgct tcagccgcta ccccgatcac atgaagcagc acgatttctt caagagcgcc
      301 atgcccgagg gctacgtgca ggagcgcacc atcagcttca aggatgacgg cacctacaag
      361 acccgcgccg aggtgaagtt cgagggcgat accctggtga accgcatcga gctgaagggc
      421 atcgatttca aggaggatgg caacatcctg ggccacaagc tggagtacaa cttcaacagc
      481 cacaacgtgt acatcaccgc cgataagcag aagaacggca tcaaggccaa cttcaagatc
      541 cgccacaatg tggaggatgg ctccgtgcag ctggccgatc actaccagca gaacaccccc
      601 atcggcgacg gcccagtgct gctgcccgat aaccactacc tgagcaccca gagcgtgctg
      661 tccaaggacc ccaacgagaa gcgcgatcac atggtgctgc tggagttcgt gaccgccgcc
      721 ggcatcaccc tgggcatgga tgagctgtac aaggagagca gcggcagctt cctgaactgc
      781 tgcccaggat gctgcatgga accgggaagc ggcaactggt cccaccccca gttcgagaag
      841 gaaaacctgt acttccagag cgattacaag gatcacgacg gcgactacaa ggaccacgat
      901 attgattaca aggacgatga cgacaagtaa gaattccgcc cctctccctc ccccccccct
      961 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     1021 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     1081 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     1141 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     1201 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     1261 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     1321 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     1381 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     1441 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     1501 cacgatgata atatggccac aacctgttac attgcacaag ataaaaatat atcatcacga
     1561 acagtaaaac tgtctgctta cataaacagt aatacaaggg gtgttatggg atcggccatt
     1621 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     1681 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     1741 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     1801 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     1861 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     1921 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     1981 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     2041 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     2101 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     2161 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     2221 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     2281 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     2341 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     2401 ttcttctgaa tctttctaga agatctccta caatttcaat attattgaag catttatcag
     2461 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2521 gttccgcgca catttccccg aaaagtgcca cctgacgtct atccgcttcc tttagcagcc
     2581 cttgcgccct gagtgcttgc ggcagcgtga agctaattcc catgtcagcc gttaagtgtt
     2641 cctgtgtcac tcaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct
     2701 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt
     2761 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt
     2821 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc
     2881 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat
     2941 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgctg
     3001 atcttcagat cctctacgcc ggacgcatcg tggccgtttt ccgctgcata accctgcttc
     3061 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt
     3121 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata
     3181 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg
     3241 gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat
     3301 gagggcaact cgagtttttc agcaaga
//
