LOCUS       62056_3235        6425 bp DNA     circular SYN 10-SEP-2019
DEFINITION  Cloning vector pAV-U6+27-Tornado-F30-Pepper-TAR Variant-2, complete 
            sequence.
ACCESSION   MN052908
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Wu J, Zaccara S, Khuperkar D, Kim H, Tanenbaum ME, Jaffrey SR.
  TITLE     Live imaging of mRNA using RNA-stabilized fluorogenic proteins
  JOURNAL   Nat. Methods 16 (9), 862-865 (2019)
  PUBMED    31471614
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Wu J, Zaccara S, Khuperkar D, Kim H, Tanenbaum ME, Jaffrey SR.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2019) Pharmacology, Weill Cornell Medicine, 1300 
            York Avenue, New York, NY 10022, United States
REFERENCE   3  (bases 1 to 6425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2019"; volume: "16"; issue: "9"; pages: "862-865"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUN-2019) Pharmacology, Weill Cornell Medicine, 1300 York 
            Avenue, New York, NY 10022, United States"
FEATURES             Location/Qualifiers
     source          1..6425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..265
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        308..581
                     /product="Tornado-F30-Pepper-TAR Variant-2"
     polyA_signal    692..813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1122..1550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1577..1681
                     /label=AmpR promoter
     CDS             1682..2539
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2713..3301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(3744..3878)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4303..4323)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(4453..4518)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(6088..6417)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gccggatcca aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat
       61 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
      121 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      181 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      241 gctttatata tcttgtggaa aggacgaaac accgtgctcg cttcggcagc acatatacta
      301 gtcgacgggc cgcactcgcc ggtcccaagc ccggataaaa tgggaggggg cgggaaaccg
      361 cctaaccatg ccgagtgcgg ccgcttgcca tgtgtatgtg ggacgcgttg ccacgtttcc
      421 cacatactct gatgatccgc tagcaaaggc tcgttgagct cattagctcc gagcccgagg
      481 taccggatca ttcatggcaa gcggccgcgg tcggcgtgga ctgtagaaca ctgccaatgc
      541 cggtcccaag cccggataaa agtggagggt acagtccacg ctctagagcg gacttcggtc
      601 cgctttttac taggacctgc aggcatgcaa gcttgacgtc ggttaccgat atccatatgg
      661 cgaccgcatc gatctcgagc cgaggactag taacttgttt attgcagctt ataatggtta
      721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc ttacgtagat aagtagcatg
      841 gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc
      901 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
      961 gggcggcctc agtgagcgag cgagcgcgca gagagggagt ggccaaagat ctctggcgta
     1021 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggctaat
     1081 gggaaattgt aaacgttaat attttgttaa tattttgtta aaattcgcgt taaatttttg
     1141 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     1201 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     1261 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     1321 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     1381 ccctaaaggg atgccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     1441 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     1501 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     1561 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2041 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2641 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2701 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2761 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2821 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2881 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2941 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3001 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcaaca cagccagctt
     3061 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     3121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagaga
     3541 tctttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3601 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagagaggga
     3661 gtggccaact ccatcactag gggttcctgg aggggtggag tcgtgacgtg aattacgtca
     3721 tagggttagg gaggtcctgg atcgatccag acatgataag atacattgat gagtttggac
     3781 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     3841 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     3901 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     3961 aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat attccttatt
     4021 aaccccttta caaattaaaa agctaaaggt acacaatttt tgagcatagt tattaatagc
     4081 agacactcta tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat aactgttatg
     4141 cctacttata aaggttacag aatatttttc cataattttc ttgtatagca gtgcagcttt
     4201 ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca cagcatgact
     4261 caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc tcttcttttt
     4321 tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg gcatttcttc
     4381 tgagcaaaac aggttttcct cattaaaggc attccaccac tgctcccatt catcagttcc
     4441 ataggttgga atctaaaata cacaaacaat tagaatcagt agtttaacac attatacact
     4501 taaaaatttt atatttacct tagagcttta aatctctgta ggtagtttgt ccaattatgt
     4561 cacaccacag aagtaaggtt ccttcacaaa gatccgggac caaagcggcc atcgtgcctc
     4621 cccactcctg cagttcgggg gcatggatgc gcggatagcc gctgctggtt tcctggatgc
     4681 cgacggattt gcactgccgg tagaactccg cgaggtcgtc cagcctcagg cagcagctga
     4741 accaactcgc gaggggatcg agcccggggt gggcgaagaa ctccagcatg agatccccgc
     4801 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     4861 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     4921 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     4981 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     5041 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     5101 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     5161 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     5221 gaacagttcg gctggcgcga gcccctgatg ctcttgtcca gatcatcctg atcgacaaga
     5281 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt cgcttggtgg tcgaatgggc
     5341 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     5401 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     5461 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     5521 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     5581 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     5641 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     5701 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     5761 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     5821 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     5881 tccataaaac cgcccagtct agctatcggc atgtaagccc actgcaagct acctgctttc
     5941 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     6001 agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc
     6061 ctgagtgctt gcggcagcgt gaagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     6121 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     6181 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     6241 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     6301 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     6361 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     6421 ccaca
//
