LOCUS       V007475                 6982 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V007475
VERSION     V007475
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        152..256
                     /label="AmpR promoter"
     CDS             257..1114
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1288..1876
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2062..2202)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(2903..3195)
                     /label="CEN4"
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      complement(3196..3649)
                     /direction=LEFT
                     /label="ARS1"
                     /note="S. cerevisiae autonomously replicating sequence
                     ARS1"
     CDS             4017..5219
                     /label="AurR"
                     /note="aureobasidin A-resistant mutant of S. cerevisiae
                     inositol phosphorylceramide synthase (Aeed et al., 2009)"
     CDS             complement(5537..5929)
                     /gene="MRP17"
                     /label="Small ribosomal subunit protein bS6m"
                     /note="Small ribosomal subunit protein bS6m from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P28778"
     promoter        6190..6590
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      6628..6815
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
ORIGIN
        1 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
       61 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
      121 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      481 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      781 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
      841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2041 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2101 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2161 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2221 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2281 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2341 cagctgggcc attctcatga agaatatctt gaatttattg tcatattact agttggtgtg
     2401 gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc
     2461 ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac
     2521 tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag
     2581 tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc
     2641 tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct
     2701 taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt
     2761 taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca
     2821 tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat
     2881 tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta
     2941 ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata
     3001 aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg
     3061 gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca
     3121 gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta
     3181 tcacctctta gagttggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc
     3241 attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa
     3301 tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat
     3361 gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc
     3421 ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa tactactcag
     3481 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac
     3541 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat
     3601 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctc gaatttcgag
     3661 aaagtgccca tcagtgttca tatttgttac aaagtggttt tctgatttgg tactactgca
     3721 gaggcgtatt ttttgcttca gttaccatag cgtaagaaca ctagcgactt ttgttcgtga
     3781 accaacagag taggatttct actgctacat ctcttaggta gttggttagt ccgatcgctc
     3841 acttttggtt gttgttaagt acttcataag tttatccttt tcctttttca cactgagcta
     3901 ctttgggtat agcttttggc ccaaggatct ttgaattttc tccaaaagta ctttatttta
     3961 tatcctacag gttgcggttt tcatatttta aaaagctttt aatcattcct ttgcgtatgg
     4021 caaacccttt ttcgagatgg tttctatcag agagacctcc aaactgccat gtagccgatt
     4081 tagaaacaag tttagatccc catcaaacgt tgttgaaggt gcaaaaatac aaacccgctt
     4141 taagcgactg ggtgcattac atcttcttgg gatccatcat gctgtttgtg ttcattacta
     4201 atcccgcacc ttggatcttc aagatccttt tttattgttt cttgggcact ttattcatca
     4261 ttccagctac gtcacagttt ttcttcaatg ccttgcccat cctaacatgg gtggcgctgt
     4321 atttcacttc atcgtacttt ccagatgacc gcaggcctcc tattactgtc aaagtgttac
     4381 cagcggtgga aacaatttta tacggcgaca atttaagtga tattcttgca acatcgacga
     4441 attccttttt ggacatttta gcatggttac cgtacggact atttcattat ggggccccat
     4501 ttgtcgttgc tgccatctta ttcgtatttg gtccaccaac tgttttgcaa ggttatgctt
     4561 ttgcatttgg ttatatgaac ctgtttggtg ttatcatgca aaatgtcttt ccagccgctc
     4621 ccccatggta taaaattctc tatggattgc aatcagccaa ctatgatatg catggctcgc
     4681 ctggtggatt agctagaatt gataagctac tcggtattaa tatgtatact acatgttttt
     4741 caaattcctc cgtcattttc ggtgcttttc cttcactgca ttccgggtgt gctactatgg
     4801 aagccctgtt tttctgttat tgttttccaa aattgaagcc cttgtttatt gcttatgttt
     4861 gctggttatg gtggtcaact atgtatctga cacaccatta ttttgtagac cttatggcag
     4921 gttctgtgct gtcatacgtt attttccagt acacaaagta cacacattta ccaattgtag
     4981 atacatctct tttttgcaga tggtcataca cttcaattga gaaatacgat atatcaaaga
     5041 gtgatccatt ggctgcagat tcaaacgata tcgaaagtgt ccctttgtcc aacttggaac
     5101 ttgactttga tcttaatatg actgatgaac ccagtgtaag cccttcgtta tttgatggat
     5161 ctacttctgt ttctcgttcg tccgccacgt ctataacgtc actaggtgta aagagggctt
     5221 aatgagtatt ttatctgcaa ttacggatac ggttggtctt atgtagatac atataaatat
     5281 atatcttttt ctttcttttt cttagtcagg attgtcgttt agcataatat acatgtagtt
     5341 tatttaatca cataccactg attatcttta gaattttata aatttttgaa ataaatgggt
     5401 ggcttttaat ggtgtctatg ttaagtgagg cttttagaat gctcttcctg ctttgtttat
     5461 tatatgtgta tgaaagatat gtatgtattt acatgtgttt gtagcgtccc cagtcaaaac
     5521 ctgtgcgcta tacctaaatg gattgataat cttcattcac taattctaaa atagacttct
     5581 tccccaaaga acggtgtaac gatgaggctc tatccagctg cttatctaaa tcaactttaa
     5641 cgatggatga tcttatgaca cggggatctt tctttaaagt tcttagaatt tcagactgta
     5701 ccgcagctga tgaatcaaac agcattaaaa agtgatatgc tcgaaaatgt ttttcctggt
     5761 ctttcttcat tattttagga agatacctta tgcccatggg tacaatgtcc ctcaccacac
     5821 ctctgttttg aataatcagt ttcccgattg tggaagacaa ttcttttgct tccaactttg
     5881 gcgcattgga gttggttatg cgaacaagtc cgatcagctc ataaagcatc ttagtgaaaa
     5941 gggtggtttt gcgttattct ttcctctgga tcctctagct ccctaacatg taggtggcgg
     6001 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca
     6061 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt
     6121 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta
     6181 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc
     6241 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca
     6301 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg
     6361 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct
     6421 tccttccagt tacttgaatt tgaaataaaa aaagtttgct gtcttgctat caagtataaa
     6481 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
     6541 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact ggtacccggg
     6601 tcgactcgag ctctagaggt taactaagcg aatttcttat gatttatgat ttttattatt
     6661 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     6721 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     6781 catgaggtcg ctcttattga ccacatctct accggcatgc cgagcaaatg cctgcaaatc
     6841 gctccccatt tcacccaatt gtagatatgc taactccagc aatgagttga tgaatctcgg
     6901 tgtgtatttt atgtcctcag aggacaacac ctgttgtaat cgttcttcca cacggatcca
     6961 cagcctagcc ttcagtcgag gc
//
