LOCUS       40924_4999        5111 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle expression vector pATM2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Kelly CL, Taylor GM, Hitchcock A, Torres-Mendez A, Heap JT.
  TITLE     A Rhamnose-Inducible System for Precise and Temporal Control of Gene
            Expression in Cyanobacteria
  JOURNAL   ACS Synth Biol (2018) In press
  PUBMED    29544054
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Kelly CL, Taylor GM, Mendez AT, Hitchcock A, Heap JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2017) Centre for Synthetic Biology and Innovation,
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom
REFERENCE   3  (bases 1 to 5111)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-2017) Centre for Synthetic Biology and Innovation, 
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..1015
                     /label=similar to ndhB
                     /note="similar to ndhB"
     regulatory      1022..1066
                     /label=Psll1120
                     /note="Psll1120"
                     /regulatory_class="promoter"
     regulatory      1067..1100
                     /label=synthetic
                     /note="synthetic"
                     /regulatory_class="ribosome_binding_site"
     CDS             1101..1817
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      1827..1936
                     /label=kan
                     /note="kan"
                     /regulatory_class="promoter"
     CDS             1937..2749
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_recomb     2753..3791
                     /label=similar to ndhB
                     /note="similar to ndhB"
     rep_origin      4124..4669
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 gtgggtcaat ccctcggttt ggcgatcgcc ttggtgtttg tcattgctgg tattgccttt
       61 aaaatttccg ctgtaccctt ccaccaatgg acccccgacg tttatgaagg ttctcccacc
      121 ccggtggtgg cttttctttc ggtgggctct aaggcggccg gttttgccgt agctatccga
      181 ttactagtga ctgcctttgg tggtattacc gatgagtggc acgttatttt caccgctctg
      241 gccgtgttga gcatggtgct gggcaacgtg gtggctttag cccaaaccag catgaaacgg
      301 atgttggcct actcttccat cggtcaagca ggctttgtga tgattggcct agtggccggc
      361 agtgaagatg gttacgccag catggttttc tacatgctca tctatctgtt tatgaacctg
      421 ggggcgttta gttgcattat tctcttcacc ctccgcactg gcagtgacca aattagtgat
      481 tacgctggtc tgtaccacaa agaccccttg ttaaccttgg gcttgagcat ttgtttatta
      541 tccttggggg gcattcctcc tctggcgggc tttttcggca aaatttacat cttctgggcc
      601 ggttggcaat cgggattgta tggcctagtc ctacttggtc tggttaccag tgtagtttcc
      661 atctactact acatccgggt ggtgaaaatg atggtggtga aggagcccca ggaaatgtcc
      721 gaagtaatca aaaattaccc ggccatcaaa tggaatttac ccggcatgcg tcccctacag
      781 gtgggcattg tcgctacttt ggttgctacc tcgctggcag gtattctggc taatcccctc
      841 tttaacctcg ccaccgattc cgtggtcagc accaagatgt tgcagacagc cctccagcaa
      901 acaggagaaa ctccggcgat cgccatttcc catgatttac cctaggggta tcaggaaata
      961 ttgctttgca ggcaaaagcc aatgagtgta actatagaaa ccgatttaaa ggagactgca
     1021 gcagtttccg gccaatggcg atcgccaagc gtcgtatatt aggcattcgg gtaagtttat
     1081 aatatacaaa ggaggtagaa atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1141 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1201 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1261 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc
     1321 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg
     1381 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1441 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1501 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1561 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1621 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1681 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     1741 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1801 tggacgagct gtacaagtaa ggatccaaag ccacgttgtg tctcaaaatc tctgatgtta
     1861 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1921 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
     1981 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     2041 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2101 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2161 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2221 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     2281 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2341 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2401 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2461 tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac
     2521 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2581 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2641 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2701 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcttgggg
     2761 gaaattaaga ccaaactcga tgacctccaa aaagatgtaa cttctcttaa gatcgatatg
     2821 gcaacggtga aaaccgagtt atctgcggtc aggatggaga taggtacagt caaggatgat
     2881 gttaaagatg tcaaagggcg ggctaatgct caaatttggg cgttgattct tgccgtcatc
     2941 ggagccataa ttaccacctt ggtgcgtttt ggcattttcc ctaatcccta acaaaaaagc
     3001 gaccaggctt ttctttcaat tgcccgatcg cctttgatat tttcccaaag gataaaagct
     3061 agtccattca gaatcgagcc ttaaagtact cccatattgg ctagccccag aattactcca
     3121 gcgccgagga tgtggccaaa gctagcggtg cccagcacag cccctaaacc aaagccgcca
     3181 aagaagttag aggaaggcat gggggtgccc acattttgtt gtttgatggt caatttacca
     3241 aaggcgatcg ccaaaatgtt gcaagcaatc atcaccccag caactttagg gctccaggac
     3301 agggtggcgg gaacggcggt ggccaacaaa aagctatgca ttgagattct ccagaataaa
     3361 gacggttttt aaagggatag ccccacgcta atgggggtct ttaaaaatct catcttacgg
     3421 ggacgctctg cccctgggaa accaccgttg caatacttaa caaattttcg tttttagctt
     3481 ggcaaatgtc tttggcaaaa ttggttgatc tggcttaaat cgtcagttat ttgccctgga
     3541 atagtctggg gacgggcaat tctgatcaga tttaccccca acgcttccgc cactttttgc
     3601 ttaaccaatt ctcccccctg ggcaccggag gctttagtta ccaccccttg aatttgccat
     3661 tgttgccaca gggctttttc caatggttcg gctacggggg ggcgcaaagc aatgatacgg
     3721 tcggaagtaa acccagcggc gatcgcctgg gctagggctt ggggataggg cagaatacgg
     3781 gcaaatagaa caaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3841 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3901 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3961 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4021 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4081 tttcgttcca ctgagcgtca gaccccttaa taagatgatc ttcttgagat cgttttggtc
     4141 tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg
     4201 ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa
     4261 aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat
     4321 caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga
     4381 cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     4441 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg
     4501 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     4561 gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt
     4621 tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc
     4681 cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc
     4741 cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc
     4801 gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc agcctttttt
     4861 ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta
     4921 tacactccgc tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc
     4981 ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt
     5041 gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
     5101 gggaagatgc g
//
