LOCUS       V009416                 4378 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009416
VERSION     V009416
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Amstutz P, Binz HK, Parizek P, Stumpp MT, Kohl A, Grutter MG, Forrer
            P, Pluckthun A.
  TITLE     Intracellular kinase inhibitors selected from combinatorial
            libraries of designed ankyrin repeat proteins
  JOURNAL   J. Biol. Chem. 280 (26), 24715-24722 (2005)
   PUBMED   15851475
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Stumpp MT, Forrer P, Binz HK, Amstutz P, Zahnd C, Pluckthun A.
  TITLE     Designed ankyrin repeat proteins binding maltose binding protein
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4378)
  AUTHORS   Forrer P, Stumpp MT, Binz KH, Amstutz P, Zahnd C, Pluckthun A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2003) Biochemistry, University of Zurich,
            Winterthurerstrasse 190, Zurich 8057, Switzerland
REFERENCE   4  (bases 1 to 4378)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4378)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
            Chem."; date: "2005"; volume: "280"; issue: "26"; pages:
            "24715-24722"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (20-JUN-2003) Biochemistry, University of Zurich,
            Winterthurerstrasse 190, Zurich 8057, Switzerland"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             127..144
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             157..486
                     /gene="D"
                     /label="Capsid decoration protein"
                     /note="Capsid decoration protein from Escherichia phage
                     lambda. Accession#: P03712"
     misc_feature    487..531
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     terminator      541..635
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      742..828
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(891..1970)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(1971..2048)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    2228..2368
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2554..3142)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3316..4173)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4174..4278)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatct ggttccatgg cgagcaaaga aacctttacc
      181 cattaccagc cgcagggcaa cagtgacccg gctcataccg caaccgcgcc cggcggattg
      241 agtgcgaaag cgcctgcaat gaccccgctg atgctggaca cctccagccg taagctggtt
      301 gcgtgggatg gcaccaccga cggtgctgcc gttggcattc ttgcggttgc tgctgaccag
      361 accagcacca cgctgacgtt ctacaagtcc ggcacgttcc gttatgagga tgtgctctgg
      421 ccggaggctg ccagcgacga gacgaaaaaa cggaccgcgt ttgccggaac ggcaatcagc
      481 atcgttggat ccggtggtac cccgggtcga cctgcagcca agcttaatta gctgagcttg
      541 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
      601 tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagcagtac tgcgatgagt
      661 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggggtaa
      721 tgactctcta gtttgaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      781 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgctctag
      841 agatttccct cgacaattcg cgctaactta cattaattgc gttgcgctca ctgcccgctt
      901 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      961 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     1021 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     1081 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     1141 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     1201 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     1261 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     1321 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     1381 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     1441 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     1501 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     1561 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     1621 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     1681 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     1741 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     1801 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     1861 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     1921 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     1981 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcacctttcg
     2041 atggtgtcaa cgtaaatgca tgccgcttcg ccttccctag ctagagctgc ctcgcgcgtt
     2101 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2161 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2221 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     2281 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     2341 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     2401 gcgctcggtc tgtcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2521 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2641 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     2761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     3001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3241 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3301 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3361 tcgttcatcc atagctgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3421 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3601 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3721 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3781 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4141 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4261 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     4321 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcac
//
