LOCUS       V009417                 4976 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009417
VERSION     V009417
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Tooley AJ, Cai YA, Glazer AN.
  TITLE     Biosynthesis of a fluorescent cyanobacterial C-phycocyanin
            holo-alpha subunit in a heterologous host
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 98 (19), 10560-10565 (2001)
   PUBMED   11553806
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Tooley AJ, Glazer AN.
  TITLE     Biosynthesis of the cyanobacterial light-harvesting polypeptide
            phycoerythrocyanin holo-alpha subunit in a heterologous host
  JOURNAL   J. Bacteriol. 184 (17), 4666-4671 (2002)
   PUBMED   12169589
REFERENCE   3  (bases 1 to 4976)
  AUTHORS   Tooley AJ, Glazer AN.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2003) Molecular and Cell Biology, University of
            California, Berkeley, 142 LSA no. 3200, Berkeley, CA 94720-3200, USA
REFERENCE   4  (bases 1 to 4976)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2001"; volume: "98"; issue: "19"; pages:
            "10560-10565"
            SGRef: number: 2; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2002"; volume: "184"; issue: "17"; pages:
            "4666-4671"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2003) Molecular and Cell Biology, University of California,
            Berkeley, 142 LSA no. 3200, Berkeley, CA 94720-3200, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      71..616
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             1211..2023
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      2790..2795
                     /label="trc promoter"
                     /note="trc promoter"
                     /regulatory_class="minus_35_signal"
     regulatory      2813..2819
                     /label="trc promoter"
                     /note="trc promoter"
                     /regulatory_class="minus_10_signal"
     misc_feature    2825..2826
                     /label="transcription initiation site from trc promoter"
                     /note="transcription initiation site from trc promoter"
     protein_bind    2827..2843
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      2852..2857
                     /gene="hox1"
                     /regulatory_class="ribosome_binding_site"
     CDS             2870..2887
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2909..2929
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             2936..3655
                     /gene="pbsA1"
                     /label="Heme oxygenase 1"
                     /note="Heme oxygenase 1 from Synechocystis sp. (strain PCC
                     6803 / Kazusa). Accession#: P72849"
     gene            3677..4436
                     /gene="pcyA"
                     /label="pcyA"
                     /note="slr0116"
     regulatory      3677..3682
                     /gene="pcyA"
                     /regulatory_class="ribosome_binding_site"
     CDS             3690..4436
                     /codon_start=1
                     /gene="pcyA"
                     /product="3Z-phycocyanobilin:ferredoxin oxidoreductase"
                     /label="pcyA"
                     /note="PcyA; catalyzes the conversion of biliverdin to
                     3Z-phycocyanobilin"
                     /protein_id="AAP45708.1"
                     /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPED
                     LGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKGLDILHCVMFPEPLYGLPLFGC
                     DIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSEYCL
                     FIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQKNDKT
                     RRVLEKAFGEAWAERYMSQVLFDVIQ"
     terminator      4713..4799
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4891..4918
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttaataa
       61 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa
      121 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta
      181 actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc
      241 atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt
      301 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac
      361 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
      421 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa
      481 ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag
      541 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg
      601 gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc
      661 ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga
      721 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc
      781 tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct
      841 catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc tgcctcgtga
      901 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag
      961 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg
     1021 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc
     1081 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc
     1141 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     1201 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
     1261 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1321 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1381 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1441 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1501 caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1561 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1621 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1681 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1741 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
     1801 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1861 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     1921 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     1981 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     2041 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata
     2101 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
     2161 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
     2221 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca
     2281 gcaacacctt cttcacgagg cagacctcag cgctcaaaga tgcaggggta aaagctaacc
     2341 gcatctttac cgacaaggca tccggcagtt caacagatcg ggaagggctg gatttgctga
     2401 ggatgaaggt ggaggaaggt gatgtcattc tggtgaagaa gctcgaccgt cttggccgcg
     2461 acaccgccga catgatccaa ctgataaaag agtttgatgc tcagggtgta gcggttcggt
     2521 ttattgacga cgggatcagt accgacggtg atatggggca aatggtggtc accatcctgt
     2581 cggctgtggc acaggctgaa cgccggagga tcctagagcg cacgaatgag ggccgacagg
     2641 aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg aacgtcgtgc
     2701 tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc agtattgccc
     2761 gctccacggt ttaattctga aatgagctgt tgacaattaa tcatccggtc cgtataatct
     2821 gtggaattgt gagcggataa caatttcaca caggaaacag accatgggtc atcatcatca
     2881 tcatcacgat tacgatatcc caacgaccga aaacctgtat tttcagggcg cccatatgag
     2941 tgtcaactta gcttcccagt tgcgggaagg gacgaaaaaa tcccactcca tggcggagaa
     3001 cgtcggcttt gtcaaatgct tcctcaaggg cgttgtcgag aaaaattcct accgtaagct
     3061 ggttggcaat ctctactttg tctacagtgc catggaagag gaaatggcaa aatttaagga
     3121 ccatcccatc ctcagccaca tttacttccc cgaactcaac cgcaaacaaa gcctagagca
     3181 agacctgcaa ttctattacg gctccaactg gcggcaagaa gtgaaaattt ctgccgctgg
     3241 ccaagcctat gtggaccgag tccggcaagt ggccgctacg gcccctgaat tgttggtggc
     3301 ccattcctac acccgttacc tgggggatct ttccggcggt caaattctca agaaaattgc
     3361 ccaaaatgcc atgaatctcc acgatggtgg cacagctttc tatgaatttg ccgacattga
     3421 tgacgaaaag gcttttaaaa atacctaccg tcaagctatg aatgatctgc ccattgacca
     3481 agccaccgcc gaacggattg tggatgaagc caatgacgcc tttgccatga acatgaaaat
     3541 gttcaacgaa cttgaaggca acctgatcaa ggcgatcggc attatggtgt tcaacagcct
     3601 cacccgtcgc cgcagtcaag gcagcaccga agttggcctc gccacctccg aaggctagtc
     3661 ttccctggcg cgccatagga gtatttcata tggccgtcac tgatttaagt ttgaccaatt
     3721 cttccctgat gcctacgttg aacccgatga ttcaacagtt ggccctggcg atcgccgcta
     3781 gttggcaaag tttacccctc aagccctatc aattgccgga ggatttgggc tacgtagaag
     3841 gccgcctgga aggggaaaag ttagtgattg aaaatcggtg ctaccaaacg ccccagtttc
     3901 gcaaaatgca tttggagttg gccaaggtgg gcaaagggtt ggatattctc cactgtgtaa
     3961 tgtttcctga gcctttatac ggtctacctt tgtttggctg tgacattgtg gccggccccg
     4021 gtggagtaag tgcggctatt gcggatctat cccccaccca aagcgatcgc caattgcccg
     4081 cagcgtacca aaaatcattg gcagagctag gccagccaga atttgagcaa caacgggaat
     4141 tgcccccctg gggagaaata ttttctgaat attgtttatt catccgtccc agcaatgtca
     4201 ctgaagaaga aagatttgta caaagggtag tggacttttt gcaaattcat tgtcaccaat
     4261 ccatcgttgc cgaacccttg tctgaagctc aaactttgga gcaccgtcag gggcaaattc
     4321 attactgcca acaacaacag aaaaatgata aaacccgtcg ggtactggaa aaagcttttg
     4381 gggaagcttg ggcggaacgg tatatgagcc aagtcttatt tgatgttatc caataaaaga
     4441 ctctccagca tgcaagcttg gctgttttgg cggatgagag aagagcagcc tgaatggcga
     4501 atggcagctt ggctgttttg gcggatgaga taagattttc agcctgatac agattaaatc
     4561 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     4621 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     4681 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     4741 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     4801 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     4861 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     4921 cgtttctaca aactcttttt gtttattttt ctaaatacat tcaaatatgt atccgc
//
